Tolerance of BMNV to ether, NaCl concentration and pH was investigated by means of the infection method of the author using larval and post-larval kuruma shrimp, Penaeus japonicus. Test shrimp sampled on day 4 after water-borne inoculation were examined for nuclear hypertrophy of the mid-gut gland epithelial cells in fresh squash preparations under the dark field microscope. The results obtained are summerized as follows;
Ether : BMNV did not tolerate to 18 hr treatment with ethyl ether at 4°C.
NaCl concentration : BMNV was inactivated in 25% NaCl solution within 10 hours and in 12.5% NaCl solution within 24 hours. But it tolerated to 24 hr treatment with NaCl solutions of 0-6.0%.
pH : BMNV had low telerance to low pH, and was inactivated in sea water adjusted to pH value of 1.0 within 10 minutes, 1.5 and 2.0 within 30 minutes, 2.5 within 60 minutes, and 3.0 and 4.0 within 180 minutes. Whereas the virus had high tolerance to high pH, and was not inactivated by 180 minute exposure in sea water adjusted to pH values of 10.0-13.0.
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