The virucidal activities of ethanol, methanol, propanol, phenol, cresol, iodophor and chlorine were examined. Infectious pancreatic necrosis virus (IPNV) was inactivated by ethanol, methanol, cresol, iodophor and chlorine but was not inactivated by propanol or phenol. Inactivation of IPNV required long-term exposure to a high concentration of cresol. Iodine and chlorine effectively inactivated IPNV suspended in PBS (-). The virucidal activities of ethanol, cresol, iodophor or chlorine were eliminated in the presence of organic substances.