Fish Pathology
Online ISSN : 1881-7335
Print ISSN : 0388-788X
Search
OR
Browse
Search
Volume 41 , Issue 3
Showing 1-8 articles out of 8 articles from the selected issue
    • |<
    • <
    • 1
    • >
    • >|
Research Articles
  • Tatsuya Kobayashi, Makoto Imai
    Volume 41 (2006) Issue 3 Pages 91-97
    Released: May 11, 2007
    JOURNALS FREE ACCESS
    Histopathology of experimentally infected ayu Plecoglossus altivelis with bacterial hemorrhagic ascites (BHA) was studied in order to determine its histopathological features. Fish (2.3 g in average weight, n=108) were infected with Pseudomonas plecoglossicida by immersion (IM) (ca. 107 CFU/mL), intraperitoneal injection (IPI) (ca. 104 and 107 CFU/fish) and intramuscular injection (IMI) (ca. 104 and 107 CFU/fish). The cumulative mortalities ranged from 92.9 to 100%. In the IM challenge groups, necrotic lesions accompanied by edema, fibrin deposition and hemorrhage were observed in the splenic and hematopoietic tissues. In some fish, the liver had necrotic lesions and microabscesses. In both the IPI and IMI challenge groups, lesions of the spleen and kidney were similar to those of the IM challenge groups. In addition, necrotic lesions and hemorrhage in the abdominal adipose tissue and pancreas were often observed in the IPI challenge groups. In the IMI challenge groups, the infected fish had definite lesions of the musculature at the injected site. The results revealed that the lesion formation in the spleen and kidney was the main pathological changes of BHA in ayu.
    View full abstract
    Download PDF (4894K)
  • Takahiro Nagai, Takashi Sakamoto
    Volume 41 (2006) Issue 3 Pages 99-104
    Released: May 11, 2007
    JOURNALS FREE ACCESS
    Three stocks of ayu Plecoglossus altivelis, viz. an amphidromous stock, domesticated stock and hybridized stock between amphidromous and domesticated stocks, were challenged by intraperitoneal injection and by field spontaneous infection with Flavobacterium psychrophilum to compare susceptibility to bacterial coldwater disease (BCWD). Cumulative mortalities of the hybrid stock were medium between those of the amphidromous (low) and domesticated stock (high). It is possible that susceptibility to BCWD was inherited to the hybrid stock. The efficacy of immersion vaccine with the formalin-killed bacterin of F. psychrophilum and the serum aggulutinin titer of fish immunized with the formalin-killed cells of F. psychrophilum or Vibrio anguillarum were compared between these stocks. Although the mortality of the vaccinated fish was significantly lower than that of unvaccinated fish for each stock, no obvious difference was observed in the efficacy of immunization between these stocks. However, the serum aggulutinin titer of the amphidromous stock against both antigens was highest among the three stocks, possibly explaining the low susceptibility of the stock to BCWD.
    View full abstract
    Download PDF (298K)
  • Tomomasa Matsuyama, Takashi Kamaishi, Norihisa Oseko
    Volume 41 (2006) Issue 3 Pages 105-112
    Released: May 11, 2007
    JOURNALS FREE ACCESS
    We developed a low-density oligonucleotide DNA array for the discrimination of 17 Vibrio and 1 Photobacterium species that are pathogenic to aquatic animals. We designed oligonucleotide probes targeting non-coding regions of the intergenic transcribed spacers (ITS) between 16S and 23S ribosomal DNA. Based on the alignment of ITS sequences obtained from the database and the results in this study, three oligonucleotide probes from a bacterial species were designed and immobilized on a nylon membrane. The ITS regions were PCR-amplified using a pair of universal primers, followed by hybridization of the digoxigenin (DIG)-labeled PCR products to the membrane. Specific signals were produced with alkaline phosphatase-conjugated anti-DIG antibody and chemiluminescent substrate. Although cross hybridization was observed, the patterns from each species were different among the species. This DNA array is useful for a rapid and easy discrimination of Vibrio species. The total process, including PCR amplification, can be carried out within a day. Our method enables a global screening of fish pathogenic Vibrio and Photobacterium species
    View full abstract
    Download PDF (2272K)
Short Communications
Notes
    • |<
    • <
    • 1
    • >
    • >|
feedback
Top