魚病研究
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47 巻 , 1 号
選択された号の論文の8件中1~8を表示しています
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論文
  • Yasuhiko Kawato, Toshihiro Nakai
    47 巻 (2012) 1 号 p. 1-6
    公開日: 2012/03/27
    ジャーナル フリー
    We introduced eight fish pathogen phages and three Escherichia coli phages (T4, T7 and λ) into the intestinal tract of goldfish Carassius auratus by anal intubation (108.3 PFU/fish). All phages appeared in the kidney at mean PFUs ranging from 102.7 to 104.3 PFU/g 3 h after intubation. Anal intubation of a representative Pseudomonas plecoglossicida phage (PPpW-4) into goldfish revealed that the introduced phages transfer to the blood and kidney within 10 min and are retained there for at least 12 h. An intubation dose of 107.3 PFU/fish or higher is required for constant presence of the phages in the circulatory system. Our results indicate that phages have the ability to pass through the intestinal wall and spread to the circulatory system, suggesting the potential application of phage therapy with oral administration.
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  • Aki Namba, Nobuhiro Mano, Hitomi Hirose, Teruyuki Nakanishi
    47 巻 (2012) 1 号 p. 7-11
    公開日: 2012/03/27
    ジャーナル フリー
    Aeromonas veronii is a Gram-negative opportunistic pathogen in fish. We have previously investigated the intestinal microbiota of carp Cyprinus carpio and revealed that most bacterial isolates with high mucus-binding capability were A. veronii. With regard to infection, mucosal adhesion of bacteria is regarded as a prerequisite for colonization and invasion. In the present study, we selected three A. veronii strains with different mucus-binding capability and pathogenicity, and compared the colonization capability and localization of these strains in the intestinal tract of carp, using an in vivo imaging system. We showed that only a pathogenic strain was mainly visible around the intestinal bulb and rectum of the intestinal tract at 48 h after administration. Frozen sections with fluorescence signals revealed that the administered bacteria were localized on the mucosal surfaces and in the lamina propria of the intestinal bulb, whereas they were mainly detected in the remaining intestinal contents in the rectum. These results suggest that the intestinal bulb is the primary region for A. veronii to invade and proliferate in healthy carp.
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  • Nao Nakajima, Mayumi Kijima, Michiko Kawanishi, Goushi Katou, Kunihiko ...
    47 巻 (2012) 1 号 p. 12-19
    公開日: 2012/03/27
    ジャーナル フリー
    We inoculated yellowtail Seriola quinqueradiata with a commercial bivalent vaccine containing inactivated Vibrio anguillarum and Lactococcus garvieae to better understand the immunological responses to the vaccination. Serum samples were collected from vaccinated and control (PBS-injected) fish at 1, 3, 5, 7, 10, and 14 days post-vaccination (dpv). Compared to the controls, the serum from the vaccinated fish inhibited the proliferation of V. anguillarum from 7 dpv. However, vaccination had no effect on L. garvieae proliferation. The antibody titer against V. anguillarum obviously increased from 7 dpv, but no such increase was detected for L. garvieae. We confirmed that the protection conferred by the V. anguillarum vaccine was a result of the antibodies against the pathogen. However, humoral factors related to protection were not observed for L. garvieae. The expression of immune-related genes in the head kidney was profiled by microarray analysis. Twenty-two immune-related genes were up-regulated during the experimental period. Up-regulation of cellular immune-related genes, such as tumor necrosis factor, CC chemokine, and macrophage colony-stimulating factor receptor, was mostly observed from 1 to 3 dpv, whereas that of humoral immune-related genes was observed from 3 to 14 dpv. Notably, immunoglobulin-related genes showed continuous up-regulation during the 2-week experimental period.
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