Objectives : Although a Japan International Corporation Agency (JICA) program was implemented to improve maternal and child care in the Republic of Benin, maternal and child mortality rates remain high. The Benin government has placed focus on developing the abilities of co-medical staff as a measure to reduce maternal and child mortality rates. In order to establish a more effective system to achieve this goal, I set out to determine job satisfaction-related factors among co-medical staff at Hôpital de la Mère et l'Enfant-Lagune in Cotonou City. Methods : In 2008, a self-administered questionnaire survey was carried out among 130 co-medical staff (21 nurses, 35 midwives, and 74 nursing assistants) at Hôpital de la Mère et l'Enfant-Lagune in Cotonou City. Fisher's exact tests and Chi-squared tests were used to examine the relationship between job satisfaction and related factors (p<0.05 was considered significant). Results : Of the 131 questionnaires distributed, 130 were collected. Marital experience was associated with job satisfaction (p=0.046). “Training” was the most often cited reason for satisfaction and the most cited suggestion for improving care. Conclusion : Qualifications, academic background, age, and years of job experience were not associated with job satisfaction. These findings suggest that job satisfaction among co-medical staff is associated with training opportunities, and that increasing the number of training opportunities may promote job satisfaction.