A new monoclonal antibody, designated WH14 antibody (WH14 Ab), was produced by using non-T ALL cell line (HBL-3 cell line) se an immunogen.
HBL-3 cell line showed characteristics of the earliest B cell committed to B cell lineage [SIg^-, CIg^-. TdT⁺, Ia⁺, B4 (CD19)⁺, B1 (CD20)^-, J5 (CD10^-, IgH^R, TcR^G], HBL-3 cell line, according to the Nadler's or Foon's classification of non-T ALL, was representative for a pre-B cell of the Stage II.
SDS-PAGE analysis of immunoprecipitates and Western blotting analysis revealed that the antigen reacting with WH14 was of molecule of 30, 000 daltons, Immunoglobulin isotype of WH14 Ab was IgGl.
In the normal hematopoietic tissue, the WH14 Ab reacted with a small numbar of monocytes (<30%) and granulocytes (<2%) in the peripheral blood, but neither with lymphocytes in the lymph nodes and tonsils nor thymocytes in the thymus.
WH14 Ab reacted with Non-T non-B cell ALL cell lines (REH) and pre-B cell line (HBL-3), but not with other B-cell leukemia/Iymphoma cell lines and EBV-transformed B cell lines.
In addition, the WH14 Ab reactd with most non-T ALLs and B lymphoblastic lymphomas (SIg^-, B4⁺, J5⁺) corresponding to pre-B cellderived neoplasm and some medium-sized cell lymphoma derived from intermediate-B cell, but not with other B-cell lymphoma.
WH14 Ab didn't react with all T-cell lymphomas including ATLL. These findings indicated that the WH14 Ab might recognize the cell surface determinant shared by immature B cells, especially pre-B eclls in the B-cell lineage.
WH14 Ab is useful for the study to analysis the characterization and difference of B cells in immature stage.

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A 61-year-old woman was admitted to Tokyo Modical College Hospital in January 1987 because of anemia and bone pain of the sternum.
Hematological findings were as follows; hemoglobin 4.7g/dl, platelet count 15.7×10⁴/μl, WBC count 2, 100/μl with 54% small lymphocytes. Bone marrow aspiration yielded a hypocellular marrow with 80.6% small lymphoid cells. A bone marrow biopsy specimen showed diffuse proliferation of small lymphocytes partially with the plasmacytoid differentiation. These small lymphocytes were found to have the surface immunogiobulin IgAκ by using immunofluorescent technique. Laboratory findings disclosed M-component of IgAκ type in the serum. Skeletal X-ray film surveys no osteolytic lesions.
From these findings, she was diagnosed as having IgAκ producing B cell malignancy diffuse lymphoma, small cell type partially with the plasmacytoid differenciation of LSG classification which origiated primarlly in the bone marrow.
This is thought to be a rare case situated between Waldenström's macroglobulinemia and multiple myeloma.

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The relationship of clinical features and pathological findings in 18 cases of adult T cell leukemia (ATL) which had been autopsied during the last eight years (1978-1985) of clinical course at Kagoshima University Hospital were analysed retrospectively. The results obtained from this study are summarized as follows.
1. Higher levels of blood chemistry, such as ALP, LAP and LDH, were commonly observed before chemotherapy.
2. ATL cells were frequently seen invading the portal area of the liver on autopsied specimens. Fibrosis of the portal area was commonly observed at varying degrees.
3. The portal area was found widened by fibrosis and/or invaded ATL cells in 10 patients. The widened portal area was not seen in eight patients, regardless of the presence of mild fibrosis or ATL cell invasion.
4. In one patient who had never received chemotherapy, except a single injection of vincristine and cyclophosphamide, the levels of ALP and γ-GTP were markedly elevated by ATL cell invasion.
5. In patients with widened portal area, the levels of ALP and LAP before chemotherapy were significantly higher than those in patients without widened portal area (P<0.05).
These results indicate that some of abnormally higher levels of blood chemistry, especially ALP and LAP, may correlate with ATL cell invasion into the portal area of the liver.

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A subcutaneous splenic implantation study was carried out using BALB/c, C57BL/6 and their F₁ mice. The frequency of heterotopic splenic regeneration under several conditions was evaluated 3 months after the implantation. An immunohistochemical examination with anti-H-2D^d and H-2D^b monoclonal antibodies was employed to analyze the derivation of the cells constituting regenerated graft tissues. A large number of the implants regenerated when the parental spleens were grafted to F₁, when BALB/c and C57BL/6 spleens were grafted to F₁, and when cyclophosphamide-treated BALB/c spleens were grafted to F₁. A very small number of the implants from lethally irradiated BALB/c regenerated on F₁. All splenic implants of F₁ to parental mice were rejected, but when the parental grafts which had regenerated on F₁ were re-implanted to the original strain of mice, more than 50% of them regenerated. The H-2 immunohistochemical examination revealed that most hematogenic cells of the parental grafts that regenerated on F₁ were replaced by F₁ host type, but the stromal cells such as endothelial cells, reticulum cells of the white pulp and connective tissue cells of the trabecula were of the doner type. The H-2 immunophenotype of follicular dendritic cells in these grafts were also of the doner (parental) type. These results indicate that requirement of the implant derived stromal cells for the heterotopic splenic regeneration, and that the cell lineage of follicular dendritic cells as non-immigrating stromal cells.

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