日本網内系学会会誌
Online ISSN : 1883-6801
Print ISSN : 0386-9725
ISSN-L : 0386-9725
31 巻, 3 号
選択された号の論文の10件中1~10を表示しています
  • 48症例の臨床病理学的検討
    三浦 寛人
    1991 年 31 巻 3 号 p. 193-206
    発行日: 1991年
    公開日: 2009/06/04
    ジャーナル フリー
    A detailed clinicopathologic and immunohistochemical study was performed on 48 cases of primary gastrointestinal lymphoma which were collected from 1976 to 1987 in our department. Of the 48 malignant lymphomas (ML), 32 developed in the stomach, 7 in the small intestine, 7 in the ileocecal region, and 2 in the rectum.
    The age of the patients ranged from 14 to 84 with the average of 62. The ratio of male to female cases was 1:1 in gastrointestinal ML. The rate of the cases diagnosed clinically as ML was low; 3% were diagnosed by endoscopy and 29% by biopsy in gastric ML. Macroscopic types of gastric ML were divided according to Sano's classification and intestinal ML according to Wood's classification. In gastric ML, 50% of the cases were the ulcerative type, and the body of the stomach was commonest site. All cases in the present study were non-Hodgkin lymphomas and the most were diffuse type histologically, and only 4% were follicular lymphomas. Following the LSG classification, 57% in gastric MLs and 75% in intestinal MLs belonged to diffuse large ML. Immunohistochemical studies revealed 46 cases (96%) of the present study to be of B cell origin. As one case of gastric ML was positive for T cell markers, MT-1 and UCHL-1, this case was considered to be of T cell origin. Another case of gastric ML was positive for lysozyme, suggesting to be of histiocytic origin. The extent of tumor stage (pathological stage) and the tumor size but not histologic type were the major determinants of prognosis.
  • 小木曽 洋一, 山田 裕, 柴田 芳実, 吉田 彪
    1991 年 31 巻 3 号 p. 207-216
    発行日: 1991年
    公開日: 2009/06/04
    ジャーナル フリー
    Murine pulmonary alveolar macrophages (PAM) form macrophage (Mφ) colonies by cultures with granulocyte-macrophage colony-stimulating factor (GM-CSF). These alveolar colony-forming cells (AL-CFC) were also radioresistant, and were maintained in mice depleted of bone marrow cells and monocytes by radiostrontium (89Sr), with or without intratracheal instillation of foreign body Sephadex G50 beads. The intratracheal exposures to either crocidolite asbestos or silica particles, however, induced a significant depletion of AL-CFC as well as decrease of PAM recovered, as compared to either untreated or TiO2-exposed animals, suggesting the toxic effects of fibrogenic particles on the self-renewal of PAM. These results indicate that radioresistant AL-CFC are putative precursors of PAM which are maintained in the pulmonary alveoli independently of recruited mononuclear phagocyte precursors.
  • 新里 雅範, 細川 暁, 金子 千之, 社本 幹博
    1991 年 31 巻 3 号 p. 217-225
    発行日: 1991年
    公開日: 2009/06/04
    ジャーナル フリー
    Langerhans cells (LCs) in the epidermis and interdigitating reticulum cells (IDCs) in the lymph nodes are said to have the same morphological and immunological characteristics. In this paper, we examined LCs and IDCs in the superficial (cervical and inguinal), hilar and mesenteric lymph nodes, and also spleens, using anti S-100 protein and OKT-6 antibodies for immunostaining. Lymph nodes and spleens taken from 16 autopsies were used for the experiments. We were able to observe LCs which were positive for both anti S-100 protein and OKT-6 in the superficial and hilar lymph nodes. However, we could not find LCs in the mesenteric lymph nodes or spleens. The immunological and morphological features of LCs were similar to those of veiled cells in the marginal sinus possibly moving from the skin to the T-zone of the superficial and hilar lymph nodes. OKT-6 positive cells were not exactly identical with anti S-100 protein positive cells, because the total number of S-100 protein positive cells was greater than that of OKT-6 positive cells. The existence of a larger number of OKT-6 positive cells in the inguinal lymph nodes than in the cervical lymph nodes may be because there are more opportunities for the lower extremities to receive different kinds of stimuli than for the head. LCs may receive some stimulation from the external environment and/or retain antigens. LCs might also be responsible for T cell dependent immune responses. Our results suggest that LCs and IDCs have slightly different immunological characteristics, and therefore, they may have some different functions.
  • 敦賀 欣一, 三河 須美子, 増田 友之
    1991 年 31 巻 3 号 p. 227-235
    発行日: 1991年
    公開日: 2009/06/04
    ジャーナル フリー
    In order to understand the splenic structure and pathognomonic changes, and the intrasplenic cell movement, it is important to clarify the intrasplenic distribution of hemosiderin-laden macrophages. Distribution of hemosiderin-laden macrophages was microscopically examined using iron-stained paraffin sections of the spleens of normal rats ranging in age from neonatal to 28 months. In addition, the liver and femoral bone marrow as well as serum iron content were also examined to determine systemic conditions of iron metabolism. Hemosiderin-laden macrophages were found in all splenic compartments, i.e., the periarteriolar Lymphoid sheath (PALS), lymphoid follicles, marginal zone and red pulp. Special attention was paid to the PALS and marginal zone in this study, although hemosiderin was mainly deposited in the red pulp. Hemosiderin-laden macrophages were rarely found until rats were 3 months old, and increased in number thereafter. According to a morphometric quantitative measurement of hemosiderin using an image analyzer, hemosiderin deposition peaked at 12 months in the red pulp and at 20 months in the PALS and marginal zone. Hemosiderin deposition in the PALS and marginal zone was one-ninth to one-fourth that in the red pulp. Hemosiderin-laden macrophages were present in the lymphoid follicles beginning at 2 months. Around the artery at the splenic hilus, hemosiderin-laden macrophages appeared even at 1 month, when hemosiderin was rarely found in the red pulp and other compartments. Hemosiderin deposition increased in the PALS even at 20 and 28 months when the deposition decreased in other compartments. Early hemosiderin deposition around the artery at the splenic hilus and increase in hemosiderin deposition with age in the PALS represent an accumulation of hemosiderin resulting from lymphatic flow from the PALS to the hilus.
  • 小野 伸高
    1991 年 31 巻 3 号 p. 237-256
    発行日: 1991年
    公開日: 2009/06/04
    ジャーナル フリー
    A total of 36 cases with gastrointestinal malignant lymphomas, consisting of 22 of stomach, 8 of small intestine, 3 of large intestine and 3 of multiple occurrence through the gut, were studied to characterize growth pattern, cytologic features and histogenesis by the use of histologic and immunohistochemical techniques.
    Phenotypically 33 cases were of B cell type and 3 of T cell type.
    Cases with high grade malignancy according to working formulation showed deeper invasion in the gut wall comparing to those of lower malignancy.
    According to LSG classification, 6 cases were of follicular and other 27 cases were of diffuse lymphoma in B cell type.
    Histologically diffuse large cell lymphoma was the most preponderant type as high as 31%.
    Immunohistochemical features of mantle zone lymphocytes with sIgM+, sIgD+, Leu-1+, Leu-8+ and ALPase+ were different from those of germinal centers with sIgM+ and CALLA+. Based on these findings, a histogenesis of primary B cell gastrointestinal lymphoma was histologically and phenotypically divided into three origins, pre-follicle center cell origin (mantle zone lymphocyte origin), follicle center cell origin and postfollicle center cell origin. Medium-sized lymphomas derived from mantle zone lymphocytes may have close similarity to those of mucosa-associated lymphoid tissue (MALT) lymphomas proposed by Isaacson et al.
  • 佐藤 武彦
    1991 年 31 巻 3 号 p. 257-265
    発行日: 1991年
    公開日: 2009/06/04
    ジャーナル フリー
    Based on analysis of hemodynamic state, it has been assumed that splenic blood flow in idiopathic portal hypertension (IPH) is elevated. The author examined by scanning electron microscopy the sinuses of spleens resected from 3 patients with IPH, as well as from 10 patients with liver cirrhosis and from 3 patients with gastric cancer as controls. In IPH, the arrangement of rod cells of the splenic sinuses was severely disordered, and the slits between rod cells were irregularly enlarged. Rod cells were also deformed and accompanied branching or process formation. The bridges, rod cells which traversed the sinusal lumen, were frequently observed. Decreased resistance in the intrasplenic circulation arising from enlargement of the slits was considered to produce increased splenic blood flow. In liver cirrhosis, the slits tended to close. Slight deformity of rod cells and some bridges were found.
  • 内田 俊毅, 木村 暢宏, 松尾 良信, 秋吉 都美, 有安 利夫, 古賀 泰裕, 大島 孝一, 菊池 昌弘, 奥村 恂
    1991 年 31 巻 3 号 p. 267-273
    発行日: 1991年
    公開日: 2009/06/04
    ジャーナル フリー
    We analyzed the expression of T-cell receptor (TcR) α, β, γ, and δ chain genes in 18 T-cell leukemic cell lines. In addition, 11αβ T-cell lines with the deletion of Jδ-Cδ locus were analyzed, using 5 kinds of Vδ gene segments. It is indicated that MOLT15, HsB2, and KE37 are at pre-αβ cell level of T-cell development, showing the specific characteristics as follows; negative expression of TcR α chain gene, deletion of Jδ-Cδ segment, residual Vδ1 and Vδ5 genes on chromosomes, volume of γ chain expression between γδ T-cell and αβ T-cell, and full length β chain message. Our results also show that the human genomic structure in TcR δ-α gene locus is 5'-Vα-Vδ4-Vαn -Vδ1-Vδ5-δRec-Vδ2-Dδ-Jδ-Cδ-Vδ3-JΨ 3'.
  • 馬場 直子, 金 秀沢, 宮本 秀明, 長谷 哲男, 中嶋 弘
    1991 年 31 巻 3 号 p. 275-281
    発行日: 1991年
    公開日: 2009/06/04
    ジャーナル フリー
    We studied surface markers expressed in 58 cases of lymphoma of the skin except ATLL by immunohistochemical staining, using the ABC (avidin-biotin peroxidase complex) and PAP (peroxidase-antiperoxidase complex) methods. 40 cases were T cell lymphoma, 10 cases were B cell lymphoma and 8 cases were non-T non-B lymphoma. 36 cases of T cell lymphoma showed a helper/inducer phenotype (CD4+ CD8-), 2 cases showed a suppressor/cytotoxic phenotype (CD4- CD8+), 1 case showed CD4+ CD8+, and 1 case showed CD4- CD8-.
    All 32 cases of mycosis fungoides showed 4B4+, 2H4-, UCHL-1+, Leu8-, Leu9-. 5 cases of these were analyzed on the reactivity to LFA-1 and VLA-4α, and all 5 cases showed LFA-1+, VLA-4α+.
    Correlation with clinical stage of T cell lymphoma and expressions of each surface marker on lymphoid cells indicated fewer positively stained cells by CD1, CD7, Leu8, HLA-DR and HLA-DQ monoclonal antibodies in the advanced clinical stage.
  • 木村 暢宏, 秋吉 都美, 瀧原 義宏, 田村 和夫, 石橋 守興, 中島 浩昭, 久野 修資, 奥村 恂, 菊池 昌弘
    1991 年 31 巻 3 号 p. 283-290
    発行日: 1991年
    公開日: 2009/06/04
    ジャーナル フリー
    We analyzed the TcR δ rearrangement pattern in 7 B-ALL/LBL and 9 T-ALL/LBL patients with δ rearranged band (s). Blot hybridization analysis of PstI digests did not reveal any Jδ1-rearranged band (s) in all 7 B-ALL/LBL, suggesting DD or VD pattern, whereas all but two cases of T-ALL/LBL showed the rearrangement involved Jδ1 segment. The analysis using 5 different Vδ genes as probes revealed that the majority pattern in B-ALL/LBL corresponded to Vδ2(D)D. There are three pattern (DD, D(D)J, and V(D)DJ) of δ rearrangement but VD in 6 adult patients with CD7+ stem cell leukemia. Therefore, it is suggested that the order of δ rearrangement is DD→(D)DJ→V(D)DJ at the earliest stage of T cell ontogeny. Furthermore, our data indicates that there is the heterogeneity of Vδ gene used at different stage of T-cell development.
  • 伊藤 雅文, 中山 敦雄, 大林 雅春, 笠井 謙次, 中川 温子, 浅井 淳平
    1991 年 31 巻 3 号 p. 291-299
    発行日: 1991年
    公開日: 2009/06/04
    ジャーナル フリー
    We reported new monoclonal antibodies (moAb), MBS1-3, that raised against long term cultured mouse bone marrow stromal cells (MBSC). They were obtained from a hybridoma prepared by fusion of NS-1 mouse myeloma cells with splenic cells of rat immunized with MBSC. MoAb MBS1 (Ig G2a) recognized the cellsurface determinant mMBS1 (M.W. 10kD) on so-called blanket cells in MBSC. Immunohistochemical analysis showed that moAb MBS1 reacted with dendritic cells in red pulp cord and marginal inner zone of spleen and in perisinusoidal space of liver. We examined bone marrow, spleen and liver which were obtained from syngenic bone marrow transplanted mice (BMT mouse) after lethal irradiation and phenylhydrazine induced hemolytic anemia mice. Dendritic cells which beared MBS1 distributed in the erythroid developing colony in red pulp of spleen, bone marrow and liver of BMT mice and anemic mice. It was suggested that the dendritic cells were highly concerned with erythrocytes proliferation. We also examined the MBSC under some kinds of cytokines stimulation. The number of moAb MBS1 positive cells were increased under IL-1, IL-3 or GM-CSF stimulation. Other two moAbs, moAb MBS2 (Ig G1) reacted with fibrobalstic reticulum cells, moAb MBS3 (Ig G1) reacted with endoth-elial cells and macrophages in MBSC. Stromal cells which beared these two moAbs were little related with hematopoiesis by the examination of BMT or anemic mice.
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