Reconstructed secondary lymphoid follicles (LFs) in lymph nodes and spleens in severe combined immunodeficiency (SCID) mice (H-2^d) were observed, which were grafted by bone marrow cells obtained from CB-17 mice (H-2^d), C3H mice (H-2^k), and Wistar rats, followed by antigenic stimulations. The secondary LFs reconstituted with bone mar-row cells obtained from CB-17 mice contained functionally active follicular dendritic cells (FDCs) capable of trapping and further retaining of mouse peroxidase anti-peroxidase as immune complex. Cell grafting study on SCID mice using C3H mouse- and Wistar rat-bone marrow cells demonstrated that FDCs in reconstituted LFs exhibited makers specific for SCID mice, but not for donar animals. These data do not suggest the bone marrow cell origin of FDCs.

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The expression of adhesion molecules of lymphoma cells were studied using immunohistochemical and flow-cytometrical techniques on 187 cases with non-Hodgkin's lymphomas (NHL) of B and T cell origin. Cell adhesion molecules used in the study were as follows; LFA-1 (CD11a/18), ICAM-1 (CD54), CD44, LFA-3 (CD58), Leu8 and VLA-4 (CDw49d). The LFA-1 was more highly expressed in T cell lymphomas (44/53; 84%) than B cell lymphomas, (50/134; 37%). The expression of ICAM-1 was observed in 75 of 134 cases (56%) of B cell lymphomas and in 18 of 53 cases (34%) of T cell lymphomas. CD44 was highly expressed in both B (109/134; 81%) and T cell lymphomas (43/53; 83%). Based on bone marrow examination of 90 cases with B cell lymphomas, bone marrow involvement was observed in 4 of 6 cases (67%) with LFA-1 and without ICAM-1, in 11 of 21 cases (52%) with ICAM-1 and without LFA-1 and in 20 of 34 cases (59%) without ICAM-1 and LFA-1. On the other hand, only 4 of 29 cases (14%) with ICAM-1 and LFA-1 showed bone marrow involvement, which is markedly lower than above groups (p<0.05). These results suggest that cell adhesion molecles might have a close relation to the extension of non-Hodgkin's lymphomas.

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Clinicopathological study of T cell lymphoma in Kagoshima prefecture, which has been known to be an endemic area of adult T cell leukemia (ATL), was performed. From 1987 to 1990, 82 patients diagnosed as T cell lymphoma were studied. These 82 patients were analysed pathologically and clinically in detail. By this study, following results were obtained.
1) Anti-HTLV-1 antibodies in sera were detected in 71(86.6%) out of 82 patients with T cell lymphoma. Almost all patients who had anti-HTLV-1 antibody were diagnosed as adult T cell leukemia/lymphoma, and their prognosis was poor comparing with anti-HTLV-1 antibody negative patients.
2) Histopathologically, 28 out of 71 patients were diagnosed as malignant lymphoma, diffuse pleomorphic type, according to Lymphoma Study Group classification.
3) The incidence of CD4+8-, CD4+8+, CD4-8+ and CD4-8- surface phenotypes was 78.9%, 11.3%, 4.2% and 5.6%, respectively. In patients whose lymphoma cells had CD4+8- antigen, the survival rate was slightly higher than those with other phenotypes.
4) In patients with T cell lymphoma who had CD30 antigen on tumor cells, the frequency of hypercalcemia was significantly lower and survival rate higher than in those without CD30 antigen.

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