Several strains of mutabile type variant were obtained by cultivating several strains of E. coli including E. coli 0-15 for a long time in broth. The present paper reports . the results of the observation on the galactose fermentation ability of the strains of the daughter type mutabile variant (M TMurase) and those similar to them (RT) which were obtained from their cultures on the media containing galactose factor (galactose, lactose and raflinose) .
The author discovered many strains of organisms, which ferment galactose with acid and gas, when the above mutabile type variants ware cultivated on the culture media containing galactose. However, such orgauisms were not present in the inoculum, but they were found in the colonies grown on the agar plate. Consequently, the author recognized that these organisms are galactose fermenting daughter type variants (tentative name) (RT), though merely considered from the outside view in the growth.
This finding also supports the claim by Ushiba and Kitasato, who reported the galactose non-fermenting character of the MT variant. As to the readiness and difficulty of the occurrence of this kind of strains, rate of the occurrence and the condition under which this kind of strains appear, separate report is scheduled to follow at a later date.

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Strains of the mutabile type variant according to Murase (M-type variant) were obtained by cultivating E. coli 0-15 for a long period of time in broth.
By cultivating this variant in the galactose peptone water (GP water), a strain of galactose fermenter with acid and gas was obtained. This strain is considered to confer with the strain reported by Kijima as a galactose fermenting daughter type variant (tentatively named) obtained from the M-type variant.
When this M-type variant grown on the galactose fuchsin agar (G. E.) or on the Endo's agar (E) was inoculated in the GP water, almost no fermentation of galactose was observed, but numerous colonies were found fermenting galactose when the organisms was transplanted from ordinary agar to the GP water. Since the above fact is a peculiar mutation phenomen, on the following investigations were conducted to clarify the cause of this phenomenon.
The M-type variant grown on G. E. or on E. also fermented galactose to such an extent that no difference can be noted from the galactose fermentation in the GP water by the M-type variant grown on ordinary agar, when it was grown in the peptone water containing galactose and glucose equivalent to the constitution of lactose, or in the peptone water containing galactose, glucose and fructose equivalent to the constitution of raffinose.
When the M-type variant, grown on the ordinary agar containing galactose to the amount of 0.01%-0.05%, was inoculated in the GP water, only very few colonies were found to ferment galactose. On the other hand, many colonies were found fermenting galactose in the GP water in the experiment with ordinary agar containing 0.5%-5.0% raffinose.
Bacterial suspensions were made from various colonies grown on E. and on ordinary agar with physiologic saline, and decimal dilutions were made from each of the suspensions. Most part of each of the dilutions was inoculated into several tubes of GP water and remaining part of them was inoculated as deep layer cultivation for the purpose of bacterial count. In the cases of the M-type variant derived from ordinary agar, the fermentation of GP water was mostly recorded in the original suspension and 10-1 dilution. Although fermentations were noted in the higher dilutions, the frequency of the fermentation and the degree of the dilution were not found related. In the cases of the M-type variant derived from E, , the corresponding bacterial count was found to be the dilution of 10-2 of the preceding case, and the frequency of the fermentation was found much lower.
In view of these observations, it was presumed that the apparently peculiar phenomenon is strongly related to the pronounce effects by galactose, lactose etc. on the bacterial body M-type variant grown on E. or on GE.. When considered furthermore, although the phenomenon seems to be due to the fact that the number of viable organism in the colonies grown on E. or on G. E. is less than those in the colonies grown on ordinary agar, the phenomenon suggests the necessity of further invesgat ions in the qualitative difference supposes to be existing between these two cases since GP water was fermented by an extremely minute amount of the brown in the peptone water containing mixture of the sugars. In addition, it was known that the effect of galactose in this repect is very strong, but that lactose is not less strong in this repect. On the contrary, the effect of rafnose is extremely weak. However, the reason for the weakness of the effect of raf inose cannot be attributed to the amount of galactose contained in the component of raffinose. Therefore, the weakness of the effect of rafliinose despite the large content of galactose is considered to be related to the chemical binding modes of the components of galactose, glucose etc.
As to the process of the appearance of the galactose fermenting strain, report is scheduled to follow at a later date.

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The action potential of the ureter was detected by the oscillograph of cathode ray type or of electromagnetic type. It was elucidated that the action potential consists of three phases, i, e. weak positive, strong negative and again weak positive. “All or none law” was found to be valid for the action potential evoked by the stimulation in the ureter The conduction velocity of the action potential was 10 mm/sec. The direction of the volley propagated in the ureter was demonstrated having no effect on the wave from of the action potential or on its conduction velocity.
The relation between the response time of the action potential and the voltage of the stimulating current was hyperbolic.

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1) A 45 years old female was suffering from painless swelling in the left upper gum since the early part of June, 1952. The case visited our Clinic in the early part of September in the same year because the swelling extended to her right cheek and submaxillar region. The case underwent excision under the diagnosis of squamous cells carcinoma.
2) A 64 years old male was suffering from swelling in the upper part of the nose accompanied by stinking nasal discharge since the early part of December of 1952. The case underwent total excision under the diagnosis of squamous cells carcinoma.
3) As for the first case, the present paper points out the necessity to be careful in the performance of various pre-and post-operative examinations. As for the second case, several remarks which should be observed on the occasion of taking samples for pathological histological sections, were made.
4) The present paper reports that, for the incision to be made in the face for this operation, _??_-or _??_-shape incision in the upper lip is favorable also in the cosmetic point of view.

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Through the investigation of the influence of parotin and MAD on the nitrogen metabolism of the chick embryo, the author attempted to elucidate the influence of these hormons on the nitrogen metabolism during the embryonal stage. The followings are the principal results of the investigation.
1) The promotion or inhibition in the development of chick embryo exercised by the addition of parotin were found mild in either of the cases, but, generally speaking, the development inclined to the inhibition side in the cases with parotin of higher concentration.
2) In the cases with the administration of MAD, the development of chick embryo was found slightly inclined to the promotion side in the initial stage of the incubation with low concentration solution. However, in the terminal stage, the development demonstrated an tendency toward rather inhibition.
3) No development promoting action was demonstrated by the administration of parotin combined with MAD.
4) In the cases of the administration of parotin or MAD, no remarkable changes in the quantity of the allantoic fluid were generally noted, and no definite tendency in this respect was observed.
5) When parotin combined with MAD was administered, the quantity of the allantoic fluid demonstrated an tendency toward decrease, though slightly.
6) The inhibitive action of the parotin on the excretion of nitrogen was found weak, but stronger inhibition was noted when the administration was made on the 5th day of incubation compared with the result obtained in the cases with the administration prior to the incubation.
7) The decrease in the excretion of nitrogen into the allantoic fluid caused by the administration of MAD was recognized more evident compared with that in the cases with parotin, though the degree was generally mild.
8) The influence caused by the administration of MAD began to appear on the 12th or the 15th day of incubation, and it disappeared on the 18th day of incubation.
9) When MAD was administered on the 5th day of incubation, the influence appeared slightly earlier compared with the cases in which the administration was made prior to the incubation, and a mild decrease in the excretion of nitrogen was recognized on the 9th day of incubation.
10) The decrease in the excretion of nitrogen into the allantoic fluid was not evidently noted in the cases with the administration of parotin combined with MAD.
In short, the development promoting action on the chick embryo by parotin or MAD was extremely weak, and even inhibiting action seemed to have been noted with the administration of these substances at higher concentrations. No definite tendency in the influence of these substances on the quantity of the allantoic fluid could be noted, although a mild tendency of decrease was recognized when these substances were administered in combination. The inhibitive action of parotin or MAD on the excretion of nitrogen, though extremely weak, was recognized even in the animals in the embryonal stage, showing a slightly stronger action in the cases with MAD. However, the decrease in the excretion of nitrogen due to the administration of parotin combined with MAD was not evident.

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The author had an opportunity to observe 46 out of 172 cases of Izumi fever occurred among the shicool children of the Azabu Primary School located Minato-ku, Tokyo in March, 1952. Of the 46 cases, 19 were treated with streptomycin, 13 with penicillin and sulfa drugs and the remaining 12 were taken as controls. Evaluations were made by the shortening of the primary febrile period, prolongation of the time interval between the primary and secondary febrile periods, frequency of the presence of the secondary febrile period, shortening of the secondary febrile period, and the betterment of the subjective symptoms in the each of the types of the disease.
As the results of the present observations, the group treated with streptomycin demonstrated the prolongation of the time interval between the primary and secondary febrile periods, shortening of the secondary febrile period, and lytic recovery from the fever, were also noted, but these were noted in the other groups as well. However, streptomycin treated group demonstrated more frequently, consequently, the above favorable change of the course was considered due to the effect of streptomycin once at least for the time being, though any precise evalution was found difficult.

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Electrocardiogram, Ballisto cardiogram, brachial arterial blood pressure wave and the femoral arterial blood pressure wave were sumultaneously recorded on 15 healthy adult males for comparative study.
1) In the femoral arterial blood pressure wave, time lags were observed in each point of the wave form. The time lag at the peak and at the dicrotic elevation is larger than that at the peak and at the initial ascending point of the wave form.
2) As to the absolute values, the values measured in the femoral arterial blood pressure wave form were generally higher than those recorded in the other wave forms.
3) The femoral arterial blood pressure waves demonstrated roughly common wave form, while the brachial arterial blood pressure waves showed various different wave forms.

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