In the clinic of obstetrics, diminution of the latter half period of parturition and prevention of the loosening and hemorrhage are of great importance. Diminution in the period required for the placental delivery and that of hemorrhage during parturition were recognized by the use of Metergin on the third stage of parturition. Following the intra-venous injection of Metergin in a dose of 1 cc immediately after the delivery of fetus, labor appeared in average of 2.7 minutes and the placenta was expelled by the labor repeated for 3.6 times in average. In average, time required was 11.7 minutes and hemor-rhage was 130 cc for the third stage of delivery and total hemorrhage during parturition was 189 cc. Cases required manual separation were 8 out of the 277 (2.9%) of the control group, while they were 15 out of the 433 cases (3.4%) received Metergin. This seemingly high percentage of manual separation among the Metergin administered group, however, was not due to the contraction nor constriction of the cervix. Some other causes which neces-sitated manual separation were recognized in them. Side effects being negligible, Metergin is considered to be highly useful for the present purpose.
Primary cancer of the liver is divided into two types, hepatom and cholangiom. In Japan, the incidence rate of the cancer of the liver is about ten times as high as that in Europe. Hepatom develops most frequently in the right lobe of the liver, while cholangiomm occurs in the left lobe. The incidence rate of hepatom is found to be about three times as high as that of cholangiom and male cases are three times as many as those of female. Very often individuals of “cancer age”, namely, over forty years are affected. Fifty three years old male and forty years old female had hepatom in the right lobe of the liver, and the partial resection of the liver was performed in a rather early stage. Unfortunately, however, the patients died. Recently, by virtue of the remarkable progress in the techniques of operation, the resection of one entire lobe of the liver with cancer has become feasible and there is aa chance for the patient to survive if the operation is performed when the hepatic function is still normal and the tumor is still localized. In most cases, however, early diagnosis is difficult because of the inconsistent first signs of the primary cancer of the liver. Especially, the cases of hepatom accompanying cirrhosis often show their symptoms only when their liver function has gone considerable disturbance. In such case, it is difficult to resect they right lobe of the liver because of the swelling. Resection of one entire lobe of the liver, therefore, should be performed before abnormal liver function becomes manifest. Life of the patient with hepatic cancer is unexpectedly short, and according to the literature, most patients die about six months after discovery of the disease.
Embryonal adenosarcom which is called as embryonal mixed tumor of kidney, Wilms's tumor etc. was first reported by Eberth in 1872. Reports on this tumor were published, thereafter, by Birsch-Hirschf eld, Lubarsch and numerous other investigators. Its occurrence in Japan, however, has been quite rare. 1. The authors could investigate an embryonal adenosarcoma formed on the left side kidney of a male infant of 3 years and 10 months old. 2. After extirpation of the adenosarcoma, the patient followed a favorable clinical course and discharged from the hospital 2 weeks after operation. 3. On histological examination, the adenosarcoma was found to be the one classifiable as Masson's type 3 (nephrogenic dysembryoma) .
Irgafen and Sulfisomidine were administered into the embryonating eggs of white Leghorn, and histopathological examinations were conducted on the thyroid-gland of the embryo. Irgafen enhanced the development of thyroid-gland, while Sulfisomidine caused the productive changes of the follicular epithelia.
An ovarian cystoma patient of blood group A, was transfused with 200 cc of reserved blood of AB group in about 300 minutes during the ovarectomy. In the course of 3 days after transfusion, slight hemoglobinuria was seen. However, the other symptoms usually seen in incompatible blood transfusion such as pain in the loin and lower extremities, precordial oppression, nausea, vomiting, fever, tachycardia, hypotension and oliguria were not noted. It was the cause of the misjudgment of blood group that rouleau formation of blood cells was mistaken for agglutination. Anti-B agglutinin titer of the patient was as low as 4 times, and this seemed to be the reason why the side-effect was so mild.
Salyrgan and glutathione were injected into embryonating eggs of White Leghorn.. The body weight of chick embryo was increased by the administration of glutathione, while it was decreased by salyrgan in higher concentrations. Glutathione failed to reduce this effect of salyrgan on the body weight. The amount of allantoic fluid was increased by the administration of salyrgan, while glutathione inhibited this effect of salyrgan. Glutathione had a tendency to increase the content of sodium in the allantoic fluid, but not salyrgan. The content of nitrogen in the allantoic fluid was increased by salyrgan but this effect was inhibited by glutathione.
Mutabile-type variants (Murase) were isolated from each of E. coli, paracoli-type E. Pcoli, so-called paracolon Aerobacter and Lactose variant of the latter two. Their galactose and lactose sensitivity were tested by the paperdisk method devised by the author's laboratory. Experimental results obtained were as follows : Mutabile-type variant of E. coli and that of lactose variant of the lactose slow fermenters equally gave growth inhibition zone in such a concentration of galactose as 0.87-1.6γ and of lactose as 3.1γ—6.3γ. Mutabile-type variant of paracoli-type E. coli, though sensitive to galactose like those mentioned above, gave no inhibition zone by lactose in a concentration as high as 10, 000γ, except that 1/23 of paracoli-type E. coli and 5/5 of paracolon Aerobacter both of which gave some incomplete inhibition zone by lactose in a concentration of 100γ—1, 600γ. Their Mutabile-type variants, however, were very sensitive to lactose if they had originated from the Lactose variants derived from the original form. It is belived, therefore, that when a Mutabile-type variant is derived from the E. coli of Lactose fermenting form, it possesses the charactors inherited from its mother cell. Thus, it seems that lactose is fermented first and galactose is freed from it acting on Mutabile-type variants as if free galactose is added from the beginning. Therefore, the growth inhibitory action of lactose appears strongly on Mutabile-type variants.
Mutabile-type variants were obtained from each 20 strains of E. coli, each 25 strains of paracoli-type E. coli and their lactose variants, as well as from each 5 strains of so-calledd paracolon Aerobacter and their lactose variants. Growth inhibiting power of raffinose was determined by the paper-disk method used in the author's laboratory. Growth ihnibiting power of raffinose on Mutabile-type variant was analized in order to know its causal relationship to the raffinose f ermentability or non-fermentability of the starting strain. Experimental results obtained were as follows : Mutabile-type variants derived from the mother-cells which ferment raffinose within a day or two -and half of the variants derived from the raffinose non-fermenting mother cells demonstrated nearly the same degree of growth inhibition by raffinose. While half of the latter group demonstrated no inhibition zone even by raffinose in a concentration of 10, 000γ/0.01 ml. Therefore, it is believed that if the original starting strain ferments rathnose, the Mutabile-type variants originated from it are influenced by raffinose more frequently than those originated from the non-fermenting one. Growth inhibitory action of galactose is seen on all the strains tested, but that of lactose is not, and that of raffinose is still more complicated. This action of galactose part on Mutabile-type variants seems to be greatly dependent on the structural complexity of the galactoside used. Further details on this problem will be discussed in another paper.
1. In the present experiment, naphthalin was administered in a dose of 1.0 mg /kg body weight on rabbits, which then were put into 4 groups of Acute Groups I (naphthalin dissolved in olive oil and fed on vitamin C deficient diet), Acute Group II (same as abovt but fed on usual diet), Chronic Group I (naphthalin powder and fed on vitamin C deficient diet) and Chronic Group II (same as above but fed on usual diet) . Then, comparisons of lingual glands (Ebner gland and Weber gland) as well as pathohistological examinations of the rabbits of above mentioned 4 groups were carried out. 2. On comparison of acute and chronic groups, changes observed on the acute groups were stronger than those of chronic groups. 3. On comparison of groups I and II, in either case of acute or chronic groups, the former presented stronger changes. Namely, the toxic effect of naphthalin is considered to be alleviated by vitamin C. 4. As for Ebner gland, changes such as irregular arrangement, cloudy swelling and destruction of the grandular epithelia, cloudy swelling of the epithelia of the secretory duct, hyperemia of the interstitial tissue, cellular infiltration, crook, divergence, disconnection and disappearance of the reticular fibers of the basal membrane etc were observed in the acute groups I and II. Further, some other changes, though mild in intensity, were also observed in group I. 5. As for Weber gland, likewise, changes such as irregular arrangement, dissociation, swelling and liquefaction of the grandular epithelia, dilatation of the secretory duct, hyperemia of the interstitial tissue, cellular infiltration, decrease in argentaffinity, swelling, crook, divergence, disconnection and disappearance of the reticulum fibers etc were observed in both acute groups I and II. Further, some other changes were observed in group I. 6. Various degenerative changes observed on the lingual glands are considered to be due to the tissue toxic secondary influence of naphthalin, namely, they correspond to the secondary direct type changes in which hypoxidosis plays an important role in their development.
1. In the present experiment, diethyl-paranitrophenyl-thiophosphate was administered on rabbits by subcutaneous injections repeated twice per week in different doses of 2, 4, 8 and 12 mg/kg body weight. Then, the animal were divided into groups of acute, subacute, chronic, fatal and survived (120 to 200 days) and investigations were made on the morphological changes produced in the liver. 2. On analysing hepatic lesions, spleen which has a close functional relationship with the liver was also investigated, and thus the presence of highly advanced hemosiderosis and fibrosis were recognized. 3. Morphological changes observed in the liver were the vacuolar degeneration, fatty degeneration and focal necrosis of the hepatic parenchymatous cells. These findings were in agreement with the disappearance of the reticular fibers or PAS stainable fibers of the hepatic capillary and were dominant in the periphery of the hepatic lobule., 4. Glycogen content of the hepatic cell was found to have reduced parallel to the degenerative changes of the parenchymatous cells and the capillary wall. 5. Appearance of the leisons in hepatic interstitium was first recognized in the loosening of vascular muscle layer accompanied by gradual fiber production of the degenerative lesions in the periphery of hepatic lobule. 6. This hepato-lobular centric fibrosis was mild but was in good agreement with the advanced myocardiac damages suggesting the importance of the influence of cardiac disorders. 7. Marked hemosiderosis was recognized on the hepatic interstitium, perilobular hepatic cells, Kupper's stellate cells and partly on the hepatic cells in the center of lobule as well as spleen and this finding was especially strong in fatal cases. Intensity of hemosiderosis, however, was not in agreement with the findings of hepatic fibrosis. 8. Varying degenerative lesions of hepatic parenchyma, hemosiderosis and hepatic fibrosis stated above are considered to be the morphological expression of the disturbances of vascular system and the metabolism of hepatic parenchymatous cell caused by the hypoxydosis due to the disturbance of radox enzym by the administration of diethyl-paranitrophenyl-thiophosphate. 9. Interstitial fibrosis of the present experiment characterized by the increase of reticular fibers appeared to be closely related with the mechanism of hydropic cirrhosis and, moreover, there was a strong resemblance between the irregular production of reticular fibers in the periphery of hepatic lobule and angiohematotoxic cirrhosis accompaning the proliferation of sinus endothelium.
As a part of the studies on the nutrients for the tissue culture of epidermal cells, the author investigated the influence of the each constituent of nutrient and its pH with special emphasis on the influence for the tissue culture of human epidermis. 1, pH of human serum collected under usual condition without being air-tight was 8.0±0.1 2. pH of the chick embryo extract diluted with the same quantity of Ringer's solution was 6.5±0.06. 3. pH of the nutrient composed of salt solution (Ringer), human serm and chickembryo extract in a ratio of 4 : 4 : 2 was 7.8±0.08. 4. Nutrient adjusted of its pH by the addition of N/10 HCl and N/10 KH2PO4 practically showed no change in provided the nutrient was hermetically sealed. While, when. CO2 gas was used, the pH of nutrient showed a tendency to increase. 5. The nutrient containing N/10 HCl and N/10 KH2PO4 in a concentration of 10% inhibited the membraneous outgrowth of the epitherial cell. Increase in the concentration.of CO2 gas, likewise, inhibited the membraneous outgrowth of the epitherial cell. 6. Epitherial cell showed poor proliferation in the nutrient of high pH concentration This poor growth of epitherial cell might also due to the toxic charactor of HCI, KH2PO4. or CO2 gas used for the adjustment of pH.
Epidermis of individuals with varying blood groups, AB, A, B, and O was grown in the nutrient containing homologous serum (taken from the same individual or from the. different individual of the same blood type) as well as heterologous serum or pooled sera, . The results obtained were as follows : 1. In the tissue culture of epidermis, the use of pooled sera in the nutrient gave the best uniform growth. 2. In the case of individual serum, homologous serum gave better growth than. heterologous one but there was no significant difference whether the serum was taken. from the same individual or from the different individual of the same blood type. 3. Heterologous serum always showed poor growth, except that AB serum occasionally showed remarkable growth promoting effect. 4. Further, there was a considerable individual difference in the growth power of epidermis.