Studies were made on the relationship between cardiac function and thiamine content in blood of rabbit following administration of large amounts of thiamine. Further, the principal cause of the acute toxic death of animals after injecting thiamine was investigated. The concentration of thiamine in blood at the convulsion period was 41-18 mg/dl, but it decreased extremely after 30 minutes (40 % of the convulsive concentrations) . No remarkable change was recognized in cardiac function at 30 mg/dl of thiamine. By the toxic dose of thiamine (90 mg/kg) convulsion and respiratory failure occured, while heart beat continued 10 minutes with slight lowering blood pressure.
As there was no direct relationship between cardiac function and thiamine contents in blood it is assumed that the principal cause of the acute toxic death of animals with thiamine is led by the respiratory paralysis.

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Large amounts of thiamine (300-700 mg/kg) was injected subcutaneously to fowl for consecutive 30 days. The following results were obtained.
1. Body weight decreased slightly.
2. The 24 hours urines after injection were collected and determined the contents of thiamine in these urines. The percentage of thiamine excreted to the amounts administered was less than 50%. The urinary excretion of 2-methyl-4-amino-5-hydroxymethylpyrimidine, component of thiamine and its oxidative product, 2-methyl-4-aminopyrimidyl-5-carboxylic acid were not observed.
3. Moderate degenerative histopathological change was detected in the liver and lung, -whereas no remarkable change in gallbladder, pancreas and spleen.
4. No appreciable effect was observed on kidney-anserinase activity of the fowl.

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Extramedullary hematopoieisis (EMH) were found in the liver and spleen in 101 cases among 629 autopsy cases of malignant neoplasm. Statistical observation, macroscopic and microscopic findings in the liver and spleen, the relationship between EMH and the bone marrow metastasis of the malignant neoplasm, the relationship between EMH and the leukemoid reaction and so on were studied.
The results are as follows.
1) The frequency of the development of EMH found in cases of malignant neoplasm is a little higher than those of the marrow metastasis.
2) The number of EMH in cases of bone marrow metastasis is four times more frequent than that in cases of non bone marrow metastasis.
3) The average weight of the spleen in cases of EMH is 20 g heavier than that in cases of non EMH. But as to the weight of the liver no great difference is found between both.
4) The degree and frequency of EMH in the liver and spleen are different in many cases. Generally speaking, EMH in the spleen is more frequent and more marked than that in the liver.
Considering the weight of both organs, the total volume of EMH in the liver is greater than that in the spleen. The stagnation and metastasis of malignant neoplasm in the liver may be partly responsible for the fact that the degree of EMH in the liver is less marked than in the spleen.
5) As to G/E ratio in the foci of EMH, cases of E>G are two times more frequent than those of G<E.
In cases of leukemoid reaction, however, cases of G>E are two timer more frequent than those of E>G conversely.
6) The f requence and degree of EMH are to some extent parallel to the extent of the metastasis in the body. It may be said that the more extensive is the extent of hematogenous and lymphogenous metastasis, the higher the frequency and degree of EMH.
7) In most cases of leukemoid reaction the bone marrow metastasis and EMH in the liver and spleen are found.
From this fact there may be the close relation between these events.
But in a few cases of leukemoid reaction there are no bone marrow metastasis and EMH. Therefore EMH is not an indispensable factor in the development of the leukemoid reaction.
8) From the above-mentioned facts, it may be said that colonisation theory, that is, that hemopoietic undifferentiated cells enter into the blood by the destruction of the bone marrow and other factors, lodge in the mesenchymal tissue in the liver and spleen and multiply there, is more appropriate.
But the decisive conclusion remains obscure and further studies into this problem are encessary.

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