In an attempt to pursue the disturbances of longitudial growth of long bones, the impacts were continuously given to the head of the femur in young rabbit and studied histochemically the consequent change of the epiphyseal cartilage with time. Results. 1. A reduction in the length of the bone became appreciable towards the 15th week, while premature closure of the epiphyseal line was demonstrable at the 31st week. 2. The glycogen content was found to be reduced at the 5th week or later. 3. The content of acid mucopolysaccharide also began to decrease later than the 5th week, and this reduction is probably responsible for disturbance of calcification and consequently of growth. 4. It may be thus concluded that the impacts given to the epiphyseal cartilage in a continuous manner has resulted in disturbance of glycometabolism in the resting cartilaginous layer toward the 5th week and consequently in disturbed growth of cartilage cells, eventually leading to disturbance of bone growth.
In the present experiment, naphthalene, which is known as a methemoglobin forming substance, was administerated to rabbits by oral application under two feeding ways of standard and V. C deficient food and by intraperitoneal and subcutaneous injection. The spleens of these rabbits were then examined histologically and the following results were obtained. 1) In the oral administration group (1.0g/kg), the rabbits fed with V. C deficient food through intragastric infusion for a long term showed edema of capsule, atrophy and rarefaction of follicles, fibrous proliferation, thickening of sinus wall and swelling of sinus endothelium. These changes were more remarkable than those fed with standard food. In the group fed for a short term under the same condition, these changes were only slight, but a similar relation between both breeding ways was observed. 2) Subcutaneous injection (1.0g/kg) for a long term resulted in, thickening of capsule, atrophy of lymph follicles, peri-and intraf ollicular edema and liquefaction, proliferation of reticulum cells, vacuolization of the nuclei, thickening of sinus wall, and edema and necrosis of red pulp of a marked degree. 3) In the naphthalene poisoning, liquef active necrosis occured from exudation to the surrounding area of peripheral arterioles of pulpa, sinus wall and intrafollicular vessels due to acceleration of permeability resulting from hypoxemia.
Comparative anatomy of various sorts of the triangles formed by cranial curvature and hypophyseal point on the cranial median section of the Cynomolgus monkey was studied craniometrically. The following conclusions were obtained. 1. In ΔAPB of the Cynomolgus monkey, ∠APB and ∠PBA showed the intermediates of the respective angles of the Orang-Utan and the Chimpanzee, and ∠PAB showed those of the Macacus fuscatus (Blyth) and the Gibbon. 2. In ΔAPN, ∠PNA and ∠APN showed the intermediates of the respective angles of the Macacus fuscatus and the Gorilla, and L PAN showed those of the former and the Gibbon. 3. The three angles of LANB lay between those of the Gorilla and the human being. 4. The three angles of LPNB lay between those of the Macacus f uscatus and the Orang-Utan. 5. The hypophyseal triangles of the Cynomolgus monkey generally seemed to resemble to those of the Orang-Utan and the Chimpanzee more respectively than those of other animals.
Nivaline is an alkaloid extracted from Galanthus nivalis L. and chemically, the same as Galanthamine. Nivaline showed potent anti-cholinesterase activity and used for a remedy of the treatment of myoparalysis clinically, such cases as poliomyelitis and myasthenia gravis etc. We have studied the relationship between the anti-cholinesterase activity and the effect of Nivaline as a remedy of the treatment of poliomyelitis. The following results were obtained. 1) Cholinesterase (ChE) activity in blood of patients suffering from acute anterior poliomyelitis following the administration of 0.25 mg, 0.5mg and 2.5mg of Nivaline is almost the same as the control. 2) In vitro, 10-4-10-5 M of Nivaline inhibited 50% of the specific and non-specific ChE activity. 3) ChE activity in brain and in liver of rat and mouse following the administration of Nivaline is almost the same as the control.
It was not until the last decade that the biochemical composition of muscles has been actively investigated. In order to know a morbid state of muscles more thoroughly, the authers carried out an animal experiment, in which the level of Na, K, mucoprotein, alkaline and acid phosphatase in the serum as well as the water content and enzymatic activities (alkaline and acid phosphatase, creatinekinase and succinic dehydrogenase) of the gastrocnemius were serially determined in animals who were preliminarily subjected to ischiatic neurotomy, fixation with Plaster cast or ligation of both the femoral vein and lymphatic vessels unilaterally to prepare morbid muscle preparations in situ. The folloving results were obtained : Ischiatic neurotomy was followed by a slight increase in Na, slight decrease in K, increase in the serum mucoprotein and acid phosphatase, marked increase in the muscle acid phosphatase as well as marked decrease in creatinekinase and succinic dehydrogenase activities. After the fixation with Plaster cast there were slightly elevated Na, slightly lowered K, increased mucoprotein, and reduced creatinekinase and succinic dehydrogenase activities were noted. With the ligation of the femoral vein and lymphatic vessels all these biological values, after the initial alteration, returned to normal range by the 2nd to 3rd week. Thus, there was a difference in response to these procedures noted between mucoprotein, acid phosphatase, creatinekinase and succinic dehydrogenase.
Two cases of acute pulmonary edema during genaral anesthesia with G-O-F (N2O: O2: Halothane) inhalation were presented in this report. Case I: A 20-year-old male was operated upon with diagnosis of spondylolysis of the fifth lumbar vertebra. Acute pulmonary edema was caused by airway obstruction due to the cuff trouble of endotracheal tube during general anesthsia. Case II: A 49-year-old female underwent radical hsterectomy with diagnosis of uterine cancer. Acute pulmonary edema was caused by probably overhydration. Etiology, symptoms, diagnosis, treatment and prognosis of acute pulmonary edema during genaral anesthesia were discussed with reference to literatures.
Stastical observations of the children with inguinal hernia in our clinic were studied. The total cases operated for inguinal hernia under 15-year-old children were 320 in number. The case were divided into 2 groups: the Ist group which included the cases treated from 1960 to 1963 was 126 and the 2nd, from 1964 to 1967, 194 in number. Comparing between the 2 groups, the operative course were both smooth, the postoperative results were the same on the both groups, the mortality was none and the average age of the 2nd group was younger than that of the Ist group. We suppose that the surgical treatment for inguinal hernia should be done in infant in future.