1) The activity of SDH in normal human placental chorionic villi began to increase from the initial period of pregnancy, reaching the maximum level at the 4th month of pregnancy. Then the activity showed a sharp decrease and increased at the 7th month of pregnancy again. However, since then the activity of this enzyme again gradually decreased indicating the minimal value at the 40th week of pregnancy. The placenta in the overterm showed a decreasing tendency in the enzymatic activity which more decreased after the 43rd week. There was a negative relationship between the duration of pregnancy and the degree of decrement in the activity. 2) In general, the activity of SDH in the placenta of late toxemic pregnancy was lower than that of placenta in normal delivery. The decrease in the activity was more striking with more severe case of toxemic symptoms. The enzymatic activity in the premature spearation of the normally inplanted placenta revealed a prominent low value. 3) The value of enzymatic activity of placenta in the stage before beg-ing of pain was found to be somewhat greater than that of the stage starting in labor. 4) The enzymatic activity in the placenta with intra-uterine fetal death was extremely low. 5) As observed in vitro, L-aspartic acid and L-ornithin, L-aspartate brought no effects against this activity of human placental chorionic villi in the early stage of pregnancy. However, the placentas of normal pregnant women at the 10th month which were given with these drugs orally revealed remarkably higher activities than thcse of controls without these drugs.
Experimental studies on mitotic behaviors and labeled thymidine uptake in respect to DNA synthesis were carried out by irradiated Yoshida ascites tumor transplanted in Donryu rats. Decreasing pattern in mitotic index was found to be the proof that time required to complete the mitosis was not altered by 1000 R irradiation. Prophase, metaphase, anaphase, and telophase seemed to proceed in their normal speed regardless of the irradiation. These processes were analysed by disintegration formula of radon to find good fitfulness though the mechanisms were to be considered otherwise. The labeled cells made their appearance earlier than mitosis indicating the DNA synthetic phase prior to mitosis. It was noted that irradiated cells incorpprated more thymidine than not irradiated cells, though precise studies were left to be done. However, these findings were to be the proof indicating the decrease of de novo thymidine synthesis in irradiated tumor cells which incorporated more thymidine by salvage path way than not irradiated cells.
The biochemical studies of inflammation are now actively performed by many authorities. In this report, the effect of anti-inflammatory agents on tissues were studied and discussed from the biochemical view point. Non-steroid anti-inflammatory agents such as Pypepyrone, Indomethacin, Mefenamic acid, Benzydamine, Oxyphenbutazone and Bucolome were used and studies on their influences to the oxidative phosphorylation and the protein biosynthesis.
A course of Vitamin B12-Coenzyme (Hycobal) 500μg was intramuscularly administered three times a week to patients with perceptive deafness and tinnitus for three weeks. The audiogram after the course were compared with those taken before the administration, by which there was threshold improvement of more than ten decibel noticed in nine cases (45 %) . Remission or improvement of tinnitus was found in four cases of them (40 %) . In nine cases of improved threshold by audiometer, five cases (56 %) were of neural deafness and thresholds of all the cases of neural deafnes (100 %) were improved. No remarkable side-action was noticed. Coenzyme-B12 (Hycobal) should accordingly be evaluated as the drug of first choice in treatment of neural deafness.