The method to measure FSH (follicle stimulating hormone) which is one kind of hypophysis hormone can be classified roughly into the following three, the bioassay, the immunoassay and the radioimmunoassay. Based on the Igarashi-McCann method, the author, using the male hypophysis, studied post mortem changes which take place in the FSH activity after elapse of time and temperature. Further, in order to study the effect of the hypophysis hormone on the blood flow of the generative organ, he injected the pituitary bodies extract to the rabbits, and by experimenting on the uterine artery with the use of electromagnetic flowmeter, he arrived at the following conclusion: 1) The activity of human FSH within 20 hours after death, when preserved at a temperature of 5°C, hardly changed during a month's time; when preserved at 18°C, the activity continued for 4 days; at 30°C, it died out on the 4 th day; at 37°C, it died out on the first day. 2) It is said that the FSH concentration in the blood increases in women after the climacterium period. The FSH concentration in the hypophysis extracted from the dead bodies also increased. This shows that the activity differs in the males and the females. 3) In the cases of the rabbits, hardly any change was observed in the uterine weight in spite of having injected human hypophysis extract. Howerver, the blood flow of the uterine artery increased the same as when HCG 1000 unit was injected, showing the contrasts of twofold value. 4) In the cases of the untreated pregnant rabbits, there was a two to sevenfold increase in the blood flow as compared with narmal rabbit.
Seizure symptoms were observed by giving vitamin B6 antagonists, each of them haying different antagonistic mechanism, to mouse. Then, the influence of the administrationn of B6 and the related substances upon the seizure, which appears by the administration of B6 antagonists, and the relationship between the seizure and 5-hydroxytryptamine (5-HT) in the brain were studied. 1) Running fit was observed, when 2-methyl-4-amino-5-hydroxymethylpyrimidine (OMP), isonicotinic acid hydrazid and DL-penicillamine were subcutaneously and intraventricularly injected. The latent period was the same in both administration methods and the intraventricular dose of onsetting the fit was 1/7 to 1/80 of the subcutaneous dose. 2. With deoxypyridoxine, cycloserine, and p-aminosalicylic acid, different symptom was observed between the two administration methods. Running fit was not observed by these B6 antagonists. 3. There seems to be no relationship among B6 antagonistic mechanism, seizure symptom and seizure onset mechanism. 4. Pyridoxine (PIN) and amino-oxyacetic acid seem to show some protective effects against B6 antagonists which cause running fit. 5. The protective effect of PIN against OMP seizure was shown with 1/10 of intraperitoneal dose, when injected ventricularly. 6. By intraventricular administration of γ-aminobutyric acid and γ-amino-β-hydroxybutyric acid, onset of OMP seizure was not prevented. 7. B6 derivatives showed only the similar effect to PIN against OMP seizure. 8. Among B6 antagonists that cause running fit, only with OMP, a remarkable decrease of 5-HT content in the brain was observed. This decrease was also observed, when seizure was prevented by the administration of anticonvulsants.
Pharmacodynamic properties of thiamine (B1) and its related substances were studied in the central and the peripheral nervous system. The results were as follows: 1. The following vasomotor actions were shown when these substances were administered intravenously to the rat: depression with 5 to 20 mg/kg of B1, elevation with 5 mg/kg of oxythiamine, heteropyrithiamine, o, s-dicarbethoxythiamine, depression followed by elevation with 10 to 20 mg/kg of those three mentioned above, elevation with 5 to 10 mg/kg of thiamine tetrahydrofurfuryl disulfide, and depression with 20 mg/kg of the same. These vasomotor actions were similar to that observed in the rabbit. 2. By intraventricular injection of Bl and its relatives to the rat with the dose of 50 to 200 μg/animal, a long lasting blood pressure elevation was observed in every case. 3. This effect of blood pressure elevation caused by the intraventricular injection of B1 and the related was inhibited by the pre-treatment with 20 μg of anti-histaminic into the ventricule. 4. The direct cause of the death induced by the subcutaneous administration of Bl and the related is supposed to be the paralysis of the respiratory muscle by the neuromuscular junction blocking. 5. The toxicity of B1 antagonist is not related to the potency of antagonistic action. 6. Intoxicated symptoms observed by the injection of B1 and the related into the ventricule were quite different from that observed by the subcutaneous injection. 7. The seizure and the death caused by the injection of B1 and the related into the ventricule were not prevented by pyridoxal phosphate and amino-oxyacetic acid. 8. The similar effects were observed with B1 and the relatives to the action with quaternary ammonium compounds. 9. The behavior and EEG observed in the adult fowl administered subcutaneously with B1 and the related were the same with those of d-tubocurarine. That is, though anesthetic-like symptom was observed, no effect on EEG was noticed. 10. As to the effect observed by subcutaneous injection of B1 and the related to chicken, whose blood-brain-barrier is incomplete, peripheral action followed by central stimulant action was observed. 11. 5-hydroxytryptamine content in the brain of the chicken that presented anesthetized symptoms by the injection of B1 and the related, was affected.
I have studied on myocardial revascularization after several vascular implantation procedure on the ischemic heart of dogs. And the studies were examined in morphological. Myocardial ischemic produced by gradual constrictors using the laminaria. The results were as follows. 1. Gradual occlusion of the coronary artery can be interpreted as the result of the evolution of intercoronary collateral anastomosis in each groups. 2. Complete obstruction of the anterior descending artery promotes obviously the formation of implanted internal mammary-coronary artery anastomosis and the size of the anastimosis vessel would be 600μ. 3. Formation of the internal mammary-coronary artery anastomosis was observed at least 38 days after implant. But the developed fibrous medial hypertrophy was observed in the internal mammary artery. 4. On the group using the non-side hold implanted internal mammary artery, the patency of the implanted vessels wasn't observed an averaged of 30 days. 5. On the method of the implantation of the vessel using the free vein graft, coa-gulated blood is apt to be produced in the vein graft.
A 33 year old woman who was living in an apartment by herself died from acute CO poisoning, a case of incomplete combustion of city gas. The CO-hemoglobin concentration was 70%. At the time of autopsy, about 80 ml of liquid blood and tip of the thumb grumose tumor were attached to the dextra fimbriae tubae. Histologically, this grumose tumoe consists of Langhans' cells and choriomic syncytium and miscarriage of one month's pregnancy was noted. However, in this case, it was extopic pregnancy and a miscarriage dextra gra-viditas tubaria ampullaris. The said liquid blood in the abdominal cavity was that from miscarriage. Further, it was noted that an IUD (Intra Uterine Device) had been inserted in the uterus, but it was impossible to clarify the relationship between IUD and tubular pregnancy. The CO-hemoglobin concentration of the liquid blood which had been observed in the abdominal cavity was 70%. This signifies that CO poisoning had stimulated tubal abortion which resulted in the 80 ml of blood observed in the abdominal cavity.