It is the present situation that there is no suitable substance which is very effectively accumulated in tumor (tumor affinity agent) . 67Ga-citrate is, however, relatively accumulated in tumor tissues, and it has been extensively used clinically Our present report dealt with the investigation of scintigraphy with 67Ga-citrate, for several kinds of malignant and benign tumors. Scintigrams of 116 patients with tumor were observed at 48 hours after 67Ga-citrate 1.5-2.0 mCi injection. Eighty-two percent of lung cancers, 71 percent of malignant lymphomas and 59 percent of laryngeal carcinomas showed positive findings. As localization of the tumors are concerned, most of them in the head and neck and the chest showed positive findings. However, owing probably to accumulation of the nuclide in intestine and bone, negative findings were frequently the results in malignant lymphoma in abdomen. 67Ga-citrate-scintigraphy should be useful for detecting tumor in the head and neck and the chest, and for determining clinical stage of malignant lymphoma. And it could be contributable to radiation therapy or surgical operation and following up of the disease in size and extent. Whol body scintigraphy could find metastases with no physical findings.
Serum ceruloplasmin values were determined by the revised method of Ravin in cases of hypoplastic anemia, iron deficiency anemia and leukemia, and clinical significance was investigated from serum ceruloplasmin level. Results obtained were as follows. 1) Clinical improvement in hypoplastic anemia was seen in patients who had high serum ceruloplasmin value, but not in patients who had low ceruloplasmin value. Acute form which showed severe clinical symptomes such as bleeding and high fever was not improved in spite of having high serum ceruloplasmin value. There was negative correlation between serum ceruloplasmin value at the time of initial examination and hemoglobin value. 2) The frequency of iron deficiency anemia from serum ceruloplasmin level was 24% in low group ; 64 in normal (normal range : 20-30 mg/dl) ; 12 in high group. 3) The frequency of acute leukemia from serum ceruloplasmin level was 18% in low group ; 22 in normal group ; 60 in high group, and the frequency of chronic leukemia was 75% in low group and 25% in normal group. Serum ceruloplasmin value in the patients of acute leukemia tended to decrease towards end-stadium. 4) The combined use of human ceruloplasmin and folic acid was effective in 10 of 17 cases of hypoplastic anemia, resulting in increase of hemoglobin and erythrocytes. In some cases, there were increase of leucocytes and platelets.
The antimicrobial activitiees of aristolochic acid were estimated, and also the inactivating effects on bacterial toxins and enzymes related to pathogenicity were investigated. 1) Nine out of 20 strains of Gram-positive bacteria includingStaphylococcus, Streptococcus, Diplococcus, Bacillus, SarcinaandMycobacteriumwere inhibited at the concentrations of 50-200 mcg/ml ; the MIC of the remaining 11 strains was higher than 200 mcg/ml. The MIC on Gram-negative bacteria (8 strains), true fungi (7 strains) andTetrahynena (1 strain) were 200 mcg/ml or higher. 2) Staphylococcal α-hemolytic toxin could be inactivated after allowing to stand for 4-6 hours in 0.1% solution of aristolochic acid. 3) The protease activity ofS. aureusdecreasd markedly after 2-4 hours of incubation with 0.1% aristolochic acid. 4) The decrease or disappearance of staphylococcal coagulase was also observed. 5) Pertussis toxin could rapidly be detoxified by the action of aristolochic acid. 6) The inactivation of diphtheria toxin and venom toxin could not be recognized.
1) The take of mouse Sarcoma 37 cells was completely inhibited by incubating the tumor cells with aristolochic acid at the concentrations of 100-200 mcg/ml for 3 hours. 2) The inhibition ratio of 40-50% was obtained with 2.5-5 mg/kg doses of aristolochic acid on the subcutaneously implanted Sarcoma 37 tumor. 3) The treatment of ascitic Sarcoma 37 tumor with 1.255 mg/kg doses of aristolochic acid proved to show a remarkable prolongation of life-span of mice. 4) The cytopathic effect on HeLa cells in tissue culture was observed in concentrations of higher than 25 mcg/ml of aristolochic acid. 5) The treatment of mice with 5-25 mcg/kg of aristolochic acid proved to increase their carbon clearance activity. 6) Some protective effects of mice against bacterial infections includingStaphylococcus aureus, Diplococcus pneumoniaeandStreptococcus pyogenescould be obtained by the pretreatment with about 50 mcg/kg of aristolochic acid. 7) The phagocytic activity of peritoneal macrophages of mice could markedly be stimulated by the administration of 10-100 mcg/kg of aristolochic acid.
The change of serum insulin level after intravenous glucose loading in dogs was studied, regarding especially to comparison between immunoreactive insulin (IRI) with and without acid-ethanol extraction in the femoral, hepatic and portal vein. The conclusions were as follows 1) By acid-ethanol extraction IRI was still detectable in the sera after free insulin was removed from the sera by gel-filtration. 2) Acid-ethanol extracted component of serum behaved immunologically similar to crystalline insulin. 3) IRI changed parallet to blood glucose level in the femoral, hepatic and portal vein after glucose loading. IRI and extracted IRI, however, exhibited a reciprocal relationship in the portal vein, but not in the femoral and hepatic vein. 4) It is not cerr tamn on the role of the circulating extractable IRI and also the rela-tion between extractable IRI and NSILA, proinsulsn or bioginsulin.
In order to prove the age differences regarding peripheral nerve regeneration, observations were made at certain interval of 5 days, 1, 3, 5, 7, 10, 12, 15, and 20 weeks on the state of regeneration following the suture of amputated tibial nerves in mature (A) and immature (B) groups of rabbits. The resulte were as follows. 1. On macroscopic, examination, there were found marked muscule atrophy of the lower extremity and decubital ulcerus at Calcaneus on the operated side in A group, but they were slight or more in B group. 2. Neuroma formation at suture area was more remarkable in B group than A group and maximus cross sectional area of neuroma indicated 3.75 times in A group and 6.21 times in B group compered with control nerves. 3. Cross section dimension was great in order size at suture area, central area at peripheral 10 mm and 20 mm. 4. A group were far inferior to B group about the increasing index of number of nerve fibers after nerve suture. Increasing index at sutured peripheral nerves after 12-15 weeks indicated 63.9-77.2% in A group and 77.5-81.4% in B group. 5. By histogram, B group was superior to A group about the number of nerve fibers over 8.0μ in diameter, They indicated 9.3% in A group and 18.2% in B group on 20 weeks after suture. 6. On histologic examination, B group showed remarkable increase of number the small regenerated fibers and nerve fibers in one Schwan's tube, and also early recovery of deformity and deviation, disappearance of vacuoles and myelination of nerve cells. 7. On thle electrophysiological study, B group were superior to A group about the recoverey of the action potential and conductions rate.
Large volume of low molecular weight dextran, gelatin modified solution, 5 per cent dextrose, lactated ringer and saline as plasma expander or E. C. F replacer have been successfully used to restore hemodynamics after hemorrhage or to prime heart-lung machine. Sporadic reports aave appeared on increased bleeding or abnormal bleeding time associated with administration of large volume of these slutions. The author discussed here hemodynamics and coagulation studies on measured blood loss from standard incisions in dogs receiving these solutions. Methods Dogs weighing from 15 to 18 kilograms were anesthetized intravenously with pentobarbital sodium. Three parallel incisions down to the fascia, 5 cm. long and 1 cm, apart, were made on one flank. The duration of bleeding and the weighed blood loss before spontaneous. arrest in three incisions were determined. After the bleeding from these control incisionss had ceased, the dogs were rapidly bled by amounts of 15 ml. per kilogram femoral artery. After withdrawal of the blood, the solution to be tested was rapidly infused via the femoral vein. Ten minutes after the infusion was completed, three similar parallel incisions. were made on the opposite flank and the duration of bleeding and weighed blood loss were again measured. Conclusions were as follows. 1. Hemodynamically, cardiac output and arterial blood pressure increased but totall peripheral resistance decreased in the group of low molecular weight dextran. The similar, but less improvement was observed in the group of gelatin. However, in the other groups. no improvement of hemodynamics were observed. 2. Bleeding from skin incisions increased markedly after administration of low molecular weight dextran. Among these solutions, 5 per cent dextrose caused the least blood loss. 3. On blood coagulation studies, Clotting time, Thrombotest, and P.R.T prolonged in low molecular weight dextran, but in other solutions were almost within normal variations. with the method employed. 4. Alterations in coagulation factors do not appeared to be sufficient to explain the bleeding. The increased capillary blood flow and deranged blood cagulation were implicated as significant factor in increased bleeding.