The microbioassay (MB) method was applied to the detection of galactose in paper chromatography. Mutabile-type variant (M) of Salmonella typhimurium or Escherichia coli, the growth of which is specifically inhibited in the presences of galactose, was used as the test organism. The paper disc was prepared from the sport of galactose on filter paper and placed on agar plate containing M (disc method) ; or the paper disc was cut into pieces and added into cup on the agar plate (cup method) . After overnight incubation at 37°C, the resulting inhibition zone around the disc or cup was measured. From the experimental results, the cup method appeared to be somewhat more sensitive than the disc method. Galactose could be detected only from the position of Rf =0.2 after the ascending chromatography using the solvent composed of n-butanol, acetic acid and water (4: 1: 5) . The end point of the positive inhibition zone in MB method was 1.2 r. On the other hand, that of the detectable spot by the color reaction (aniline phthalate) was 0.9 r. When galactose solution was mixed with glucose having near Rf value, the size of inhibition zone was almost unchanged, the position of galactose on the filter paper being able to be differentiated from that of glucose by the combination of MB method and color reaction The MB method proved also to be applicable to the thin layer chromatography of galactose.
In our Department of Surgery, longterm intermittend hemodialysis has evolved for chronic renal failure as well as in our country for these few years. I intend to explore factors which contributed to prolonging the median survival of patient with chronic renal failure. Studies performed to examine hemodialysis for twentynine patients with chronic renal failure, from January 1968 to December 1973, and parted them, shorter survival group (died within three months after first hemodialysis), longer survival group (lived more than six months after first hemondialysis), compared them about chemical analysis of blood and circulatory dynamics respectively. Our data demonstrated that adequate intake and output of fluid was significant factor for prolonging the median survival of them. Therefore, considering administration of fluid, I confirm that a program of hemodialysis for them should be kept their cardiothoracio ratio under 55%.
It was studied that rabbit lung lesions and that cross-reactivities between lung tissue and microorganismus, e.g., Strept. haemolyticus, Kleb. pneumoniae, E. coli, Clost. welchii and Entero. faecalis The results were as follows; 1) In rabbit immunized with the disrupted bacteria, it was observed the marked granulomatous lesions with epitheloid cells, lymph cells and giant cells 2) On fluorescent antibody technique, the specific stainings were found at the lung and heart by the anti-Clost. welchii sera and at the kidney by the anti-Strept. haemolyticus sera 3) Auto-antibodies (anti-lung antibodies) were observed in anti-rabbit sera immunized with Kleb. pneumoniae and Entero. faecalis. 4) In passive haemoagglutination and inhibition test, there was some cross-reactivity between lung tissue and Entero. faecalis. 5) Rheumatoid factor-like substances were found in all anti-rabbit sera immunized each microrogranismus.
Ophthalmofundoscopic tests were performed on 696 cases of hypertensive patients of ages ranging from the thirties up to the seventies and the following results were obtained. The examinations of f undus oculi were conducted simultaneously on both eyes and among the cases showing differences in the findings of f undus oculi between right and left eyes, those demonstrated such different changes as right fundus Group II b and left fundus Group III according to the Keith-Wagener classification method modified by Keio University, were found in 13.1% of the 696 cases. Changes in f undus oculi tended to increase with aging but any definite correlation could not be found between plasma lipids level and fundus findings. Extravascular leakages of fluorescent pigments which are found in fluorescent opthalmo-fundoscopy were observed in none of the cases classified according to the fundus findings into the Group I, in 7.1% of the cases classified into the Group II a, in 70.6% of the cases classified into the Group II b, and in all the cases classified into the Groun III and IV.
The inner circumference of 47 arteries from 152 human adults (male: 71, female: 81) was measured and the age-related changes were examined. The materials used in this studies were obtained from the autopsy subjects in Tokyo Metropolitan Medical Examiner Office and were examined in the non-fixed situation. 1) The increasing tendency of circumference with age was remarkable for the all parts of the aortae in the male and the all examined arteries except those of abdominal organs in the female. The differences of the circumference between the age of seventy and twenty were statistically significant in these arteries. 2) The A. renalis in the male and A. mesenterica superior and inferior in the female showed a decreasing tendency of circumference with age. 3) The increasing tendency of circumference with age were middle grade for Tr. brachiocephalica, A. carotis communis, A. subclavia, A. axillaris, A. iliaca communis, A. iliaca externa, A. poplitea, A. tibialis anterior and posterior in the male. But the dif-ferences of circumference between the age of seventy and twenty were statistically insignifi-cant in these arteries. 4) In the male A. femoralis, A. carotis interna and externa, and A. brachialis show-ed increasing tendency of circumference with age slightly. 5) In the male A. facialis, A. ulnaris, A. radialis and the arteries in the brain, the age-related changes of the circumference were not seen. 6) From these facts, it can be said that the age-related changes of the circumference of the arteries have very intimate relation with the histological composition of the artery wall and its age-related changes.
It has been generally accepted that the incidence of complication of gastric ulces in cases of cirrhosis of the liver (C.L. abbreviated as shown below) is relatively high. The author studied the incidence of gastric ulcer of C.L. statistically and its pathogenesis histologically, especially based on vascular changes of the stomach. The results are as follows : 1) Among 2, 533 autopsy cases of the Second Department of Pathology, Showa University School of Medicine and three other hospitals during 10 years from 1965 to 1974, cases of C.L. came up to 115 ones (4.54%) . Out of 115 cases of C.L. the complication of gastric ulcer was revealed in 24 cases (20, 87%) . 2) Gastric ulcer concomitant with C.L. were relatively small in the size (below 2 cm in diameter), situated in the fundic gland area along the lesser curvature and shallow from UL-II to UL-III. Multiple lesions were met in more elderly persons the single one. 3) Both the liver and spleen in cases of C.L. with gastric ulcer were lighter in weight than those in cases of C.L. without gastric ulcer, the reason of which remained unexlained. 4) Histopathological studies revealed that venous changes including chronic congestion, marked dilatation, thickening of the wall and so on, seen in the gastric mucosa and submucose were the most prominent in cases of gastric ulcer with C.L. Venous changes of the stomach in cases of C.L. without gastric ulcer and of gastric ulcer surgically exstripated were in lower degree. 5) As the conclusion these venous changes in the gastric mucosa and submucosa following portal hypertation may be the most important pathogenetic factor in the development of gastric ulcer in cases of C.L.
At present, to estimation of antistreptolysin-O (ASO) titer, Rantz-Randall's method has been usually used in routine laboratory. The Rantz-Randall's method, however, is cumbersome, time-consuming and requires more skillful technics, too. Recently, a new method for estimation of ASO titer with streptolysin-O combined polystyrene-latex reagent had been developed by Behringwerke and we have investigated it. In our examination, we have compared with the titers between Rantz-Randalls' and new method and examined the relationship between the results of CRP, RA, and betalipoprotein index and the results of this new method. Our conclusions were as follows: Samples which obtained above 250 or under 166 Todd units showed 96.6% and 31.4% positive, respectively, by this method. No relationship between the results of CRP, RA, and beta-lipoprotein and this method was observed. And so, it is found that this new method is very suitable screening test for ASO by the reason of it's simplicity and rapidity.
Refampicin ointment in white vaselin base (1%) showed a satisfactory therapeutic result in 44 cases of herpes zoster. The efficacy was rated on the improvement of vesicle and a relief of herpetic pain. After 6 days of the treatment, about 80% of cases showed the formation of a crust and about 80% had a complete dryness of the vesicles in 12 days. Postherpetic neuralgia remained unimproved in 4 cases, but about 80% of the cases were relieved of pain and 70% were completely freed from the pain in 12 days. The above findings indicate that this drug may shorten the treatment period as compared with other topical drugs available.