巻号一覧
後続誌
37 巻 (1977)
- 6 号 p. 511-
- 5 号 p. 405-
- 4 号 p. 309-
- 3 号 p. 217-
- 2 号 p. 103-
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37 巻, 5 号
選択された号の論文の13件中1～13を表示しています
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鶴岡 延熹1977 年 37 巻 5 号 p. 405-410
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーPDF形式でダウンロード (951K) -
―特に低体重出生児を中心とした胎盤について―山口 修1977 年 37 巻 5 号 p. 411-423
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーFrom the comparative study of patho-histological investigation into the relations of SFD to AFD and LFD fetal placentas, especially in the case of SFD fetal placenta where placental factors are most significant, we have concluded as follows:
(1) SFD, AFD and LFD placentas all increase both in volume and weight with the progress of pregnancy between 28 and 32 weeks. An arrested development of SFD fetal placenta is observed, however, starting between 33 and 35 weeks and a tendency of SFD AFD LFD is shown in 33 weeks of fetal age and thereafter, increase the weight differences as the fetal age advances.
(2) Mutual relations in placental ratio are found in the order of SFD, AFD, LFD in 33 weeks and thereafter. It is also observed that the ratio decreases as the fetal age increases.
(3) The fetal placenta of SFD fetus from placental factors is more highly occupied by the profundus chorionic blood vessels and the VSM formation is suppressed as compared with those of SFD fetus from maternal factors or of AFD and LFD fetuses.
(4) Fibrosis of villus stroma occurs in the chorionic region among the chorionic stem and branches and some of the villi from earlier stages in the placenta of SFD fetus from fetal factors than in other cases such as SFD fetus from maternal factors, AFD and LFD fetuses.
(5) Patho-morphological observations described in (3) and (4) compose the two main characteristics in the fetal placenta of SFD fetus from placental factors.
(6) Observations in (3) and (4) are both considered to be patho-morphologically serious as the obstructional factors of blood vessels causing functional disorder between the mother and the fetus.
(7) We have conjectured from the above observations that the high occupancy by the profundus chorionic blood vessels, a suppressed VSM formation and an early fibrosis of villus stroma in the placenta of SFD fetus from placental factors are closely related to the delay of prenatal development.抄録全体を表示PDF形式でダウンロード (6213K) -
福留 厚1977 年 37 巻 5 号 p. 425-435
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーFot the determination of lactose by galactose-oxidase peroxidase (GOP) method, it is necessary to measure the amount of galactose liberated after the hydrolysis of lactose.
(1) Hydrolysis of lactose-Lactose was hydrolyzed with 0.2% H2SO4or with living cells of Escherichia coli3-MT (a mutbile-type variant which decomposes lactose but not galactose) . By H2SO4method, the calculated dose of galactose was liberated after 2 hours. By MT method, free galactose increased rapidly after 2 hours and reached maximum after 3 hours. The volume of lactose solution was diluted to 1: 4 in the procedures of acid hydrolysis and neutralization, but not in MT method.
(2) Determination of galactose liberated from lactose after hydrolysis- (i) Free galactose in the H2SO4hydrolysate of standard solution of lactose was measured by GOP or a cup-plate method using M (galactose-sensitive mutant of enteric bacteria) as the test organism. The amount of galactose was measurable in the range of 25-200γ/2 ml in the case of GOP method, and 1.25-10γ/0.1 ml in the case of M-cup method. (ii) Galactose in the MT hydrolysate was measured by GOP or M-cup method. The amount of galactose could be estimated in the range of 25-100 γ/2 ml by GOP method, but the volume of MT hydrolysate was diluted to 1: 8 because of the necessity of deproteinizing. On the contrary, the amount of 1.25-10γ/0.1 ml could be estimated by M-cup method without the need of deproteinizing.
(3) Determination of lactose in the biological material- (i) Free galactose in the H2SO4 hydrolysate of milk was measured by GOP or M-cup method. Although deproteinizing of the hydrolysate was necessary, the determination of galactose in the range of 25-100γ/2 ml was possible by GOP method. By M-cup method, 1.25-10γ/0.1 ml of galactose could be determined without the need of deproteinizing. (ii) The amount of galactose in the MT hydrolysate of milk was measured by GOP or M-cup method. While the estimation of 25-100γ/2 ml of galactose was possible by GOP method after deproteinizing, 1.25-10 γ/0.1 ml of galactose could be estimated by M-cup method without deproteinizing.
From these experimental results, the combination of MT hydrolysis and M-cup method proved to be most sensitive for the determination of lactose in the biological material.抄録全体を表示PDF形式でダウンロード (1281K) -
太田 秀典1977 年 37 巻 5 号 p. 437-448
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーUsing 41 autopsy cases of alcoholism, pancreas, liver, kidney and heart were studied histopathologically. Furthermore, using 50 rats drinked 20 % alcohol duaring 10 weeks (8 rats), 50 weeks (18 rats), and the most longest group (21 rats) drinked untill death, were studied with the same organs and the following conclusion were obtained.
1) Cirrhoric liver was found 16 cases (39.0%) of the alcoholism 41 cases and all of nothing in the no-alcoholic 40 cases.
2) Cause of death in alcoholism was found 58.5% in disease of the digestive organs. (no-alcoholism was found 10%.)
3) Cause of death in alcoholism was found 44% in the malignant tumor. (no-alcoholism was found 17.5%.)
4) Histopathological alcoholic pancreas was found the vacuole formation of the protoplasm and nucleus.
5) In the liver of alcoholism, it was found the remarkable fatty degeneration and the fibrous hyperplasia as compared with no-alcoholism, but the kidney and heart of the alcoholism was not found the remarkable changes.
6) In the pancreas of the alcoholic rats, the protoplasm and nucleus of acinous cells was found the degeneration changes (vacuole formation, atrophy, and collapse, ) . Furthermore, the collapse of acinous-structure was found here and there as sporadic changes, and as fainary changes it was found that the pancreas got in to the atrophic degeneration. Those tindings gradually become severe tollowing an increase in the quantity of alchohol intakt.
7) In the liver of alcoholic rats, was found the dissociation of the hepatic cell cord and the slight fatty degeneration, but coud not found the fibrous hyperplasia.
8) In the kidney of 10 alcoholic rats (long alcoholic group 3 cases 17%, limitid long alcoholic group 7 cases 33.3%), was found the calcification and had no-changes in the heart.
In the present autopsy investigation, it was found that the pancreas and liver of alcoholism had differrent many changes from those of no-alcoholism in statistical and histopatho-logical investigation. In the experimental results, it was found that the alcohol affected hystotoxically for the cells of pancreas especially. So I had come to the conclusion that the alcohol acted upon especially the pancreas as metabolic impediment.抄録全体を表示PDF形式でダウンロード (3936K) -
丸山 七朗, 蜂須 貢, 岩永 弘秋, 猪 好孝, 小笠原 定則, 山田 重男1977 年 37 巻 5 号 p. 449-454
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーAntidotal effects of α-mercaptopropionyl glicine (MG) and 6, 8-dithioctanic acid (lipoic acid; LA) on mercuric chloride-intoxicated mice and histological examination were investigated. Results are as follows.
After administration of mercuric chrolide in the mice were observed for 7 days. The LD50was caluculated by the method of Litchfield-Wilcoxon. The LD50in the mice was 7.7 mg/kg intravenously. Acute toxic symptoms were observed in mice, depressed of spontaneous movements, piloerection, mydriasis, salivation, tremor convulsion, no righting reflex, depressed respiration. Histological findings of kindney in the mercuric chloride treated mice were noticed degeneration of hyaline droplet, hyperemia of cortex and medulla, dystropic calcification in proximal convoluted tubule and Henle's loop.
Antidotal effects of MG and LA on mercuic chloride intoxicated at doses of LD50were noticed decrease of mortality when administrated of combination with mercuric chloride and MG or LA.抄録全体を表示PDF形式でダウンロード (3800K) -
千住 紀, 五味 邦英, 石井 暢, 矢沢 正隆, 高木 智恵子1977 年 37 巻 5 号 p. 455-460
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーIn present paper, isoenzyme anomalies of lactate dehydrogenase in two cases are reported.
One case is a healthy young man and the other case is a 53 aged male patient carrying rectal cancer.
Biochemical and immunological investigations for both LDH-anomalies were performed and different properties have been found, respectively.
Case 1;
LDH-anomalies on electrophoretic pattern were to show nondetectable LDH2, broad LDH3, and specified abnormal fraction between LDH3and LDH4.
These anomalies of this case have been originated from the cause of binding of LDH subunit to IgA-κ type. This binding was more specific to LDH2, but had not species specificity.
Case 2;
This main anomaly showed abnormal extra-band between LDH3and LDH4. This band was stable in heat (56°C, 30 min) or in 2 M urea, and had high molecular properties, but could not be prove binding to ordinary serum proteins.
This abnormal high molecular LDH isoenzyme had shifted to normal molecular size by action under stronger ionic strength. Electrophoretic mobility of dissociated LDH isoenzyme, however, was as same as originated high molecular one.
LDH isoenzyme patterns in red blood cells and urine from the patient were almost normal.
It was very interesting that this abnormal pattern was disappeared at 71 days after its the first noticed.
As above results, the authors speculate that this abnormal LDH isoenzyme consists of a few subunits with ionic bond and its binding is transient.抄録全体を表示PDF形式でダウンロード (3479K) -
飯島 武1977 年 37 巻 5 号 p. 461-468
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーArginase was purified about 800-fold from the supernatant of rat liver homogenate and subdivided into four isozymes by isoelectric forcusing. The values of isoelectric point of these four isozymes were estimated to be pH 9.3, 9.2, 9.0, and 8.8, respectively. Manganese ion was released from enzyme molecules during the procedure of isoelectric forcusing. After addition of Mn2+, the enzyme activities of each pI isozymes were increased about 2 times than that of original activities. The pI 9.0 isozyme was most stable for heat-inactivation at 60°C among these pI isozymes. The apparent Km value of arginase for L-arginine was calculated to be 20 mM, but the values of pI isozymes were to be 29 mM (pI 9.2 and 9.0 isozymes) and 32 mM (pI 9.3 and 8.8 isozymes) . The molecular weight of the arginase was estimated to be approximately 120, 000 by Sephadex G-100 gel filtration, but the molecular weight of pI isozymes were between 90, 000 to 95, 000. L-Ornithine (20 mM) and L-proline (20 mM) inhibited the enzymatic activities of pI isozymes, but carbamyl phosphate (20 mM) had no effect.抄録全体を表示PDF形式でダウンロード (2136K) -
米倉 正博, 佐藤 滋, 伊藤 正吾, 志和池 成世, 菊池 祥治, 杉崎 徹三1977 年 37 巻 5 号 p. 469-477
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーExperimental allergic interstitial nephritis was induced in Lewis rats, which were immunized with isologous kidney suspension.
The immunization was performed by the intracutaneous and intraperitoneal injections of the 50% kidney suspension, emulsified in complete Freund's adjuvant (Perrin's modification) . Pertussis vaccine was also injected into the dorsum of feet and into the peritoneal cavity. Rats were nephrectomised or sacrificed at 7, 10, 14, 21, 28, 35, 42, 77, 84 and 91 days after the immunization.
Clinical examination was performed by measurement of urinary protein, sugar and volume. Urinary volume and protein remained within normal range and no rats developed glucosuria through the period of the experiment. The susceptibility of the interstitial nephritis was found in the younger rats than the older.
By light microscopical study, the infiltration of mononuclear cells and polymorphonu-clear leucocytes (PMNs) were found locally around the tubules (mainly proxymal convoluted tubules (PCT) ) at 10 days after the immunization and with time became diffuse throughout the renal cortex. The lesion almost abolished at the 35 th day.
By direct immunofluorescent study, rat-IgG and C 3 was observed along glomerular capillary loops in granular fashion at the 35 th day.
By electronmicroscopical study, infiltration of cells (predominantly lymphocytes) were found in the interstitial tissue and some of them attached to the basement membrane of the PCT at 10-14 th day after the immunization. Other abnomalities were those, such as focal wrinkling of tubular basement membrane (TBM), gaps formation between the TBM and the tubular cells, invasion of the lymphocytes inbetween the tubular cells and/or the tubular lumen, decrease or loss of infoldings of the PCT, presence of fat droplets in the tubular cells and swelling of the mitochondrias. The lesion seems to be abolished at the 28 th day, and almost disappeared except for fibrosing changes at 35 th day. On the other hand, at the 35 th day, glomerular basement membrane (GBM) showed irregular thickning which was composed of subepithelial dense deposits.
These findings suggested that the interstitial nephritis was developed by mechanism of cellular immunity, e. g. the lymphocytes sensitized with certain renal antigen (s) may play a major role for tissue destruction and induction of infiltration of macrophages, PMNs and the other cells.抄録全体を表示PDF形式でダウンロード (7236K) -
鈴木 一, 刑部 尚美, 山村 光久, 益海 信一朗, 松本 博光, 井上 健, 中神 和清, 河本 英世, 里見 智正, 野口 英世, 小 ...1977 年 37 巻 5 号 p. 479-483
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーA case of postrubella encephalitis accompaning by delirium, exanthema, and hyperaesthesia was reported.
The patient, 40 year-old male, completely recovered by the administration of steroid, antibiotics and γ-Gl.
In the labolatory findings of this case, the serum IgE levels measured by the radioim-munoassay (sephadex method) were 2300 IU/ml and 1040 IU/ml (normal value 1000 IU/ml) . The cerebrospinal fluid specimen disclosed antirubella antibody 1: 32 by hemoagglutination inhibition titer.抄録全体を表示PDF形式でダウンロード (453K) -
佐藤 元助, 吉田 和正, 内田 伸昭, 仁木 寛, 長村 洋, 三石 績, 世良田 和幸1977 年 37 巻 5 号 p. 485-488
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーA 13-year-old girl was anesthetized two times with 10 days interval for the operation of abdominal wall abscess. At the first time anesthesia, no adverse reaction was observed, but as the second time anesthesia, 10 days later, erythama, local heat and slightly decrease blood pressure was noted, probably caused by Low Molecular Weigh Dextran.
In the recent past two and half years, among 3240 anesthetic cases whom dextran were administered, such kind of side effect developed in 38 cases.抄録全体を表示PDF形式でダウンロード (1737K) -
―刺激伝道系の組織学的検索―由利 弘一郎, 林 正博, 石井 靖夫, 新谷 博一, 高杉 キミ, 高杉 正雄, 遠藤 公平, 新井 治男, 風間 和男, 田代 浩二, ...1977 年 37 巻 5 号 p. 489-495
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーThe clinical and histopathological findings were investigated in a case of Sick Sinus Syndrome (SSS) .
Clinically he showed brady-tachycardia, S-A block, complete A-V block and atrial flutter-fibrillation for 7 months.
Pathologically there was observed the severe fibrosis in the sinus node and junction (sino-inter-nodal tracts) . This pathological findings were seemed to be related, at least in part, to the physical process of aging and also, to His bundle. The advanced fibrosis was recognized at the distal part, branching portion of posterior and anterior left bundle branch.抄録全体を表示PDF形式でダウンロード (7145K) -
野津 立秋, 矢口 勇, 坂本 言方, 須沢 忍, 横山 新一郎, 鶴岡 延熹, 清水 盈行1977 年 37 巻 5 号 p. 497-503
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーA 28-year-old woman with oppression on chest, nausea and hemoglobinuria was found to have severe anemia and agglutination of red blood cells, and was diagnosed as Evans type IgG auto-immune hemolytic anemia.
Blood studies on admission: hemoglobin 3.1 g/dl, erythrocytes 590000/cmm, hematocrit 10 %, reticulocytes 366‰, thrombocytes 72500/cmm, indirect bilirubin 3.5 mg/dl, LDH 1170 unit and red blood cells survival time of51Cr was 4 days. Direct and indirect antiglobulin tests were positive, antibody was IgG of λA type, cold agglutinin titer 256 times and Donath-Landsteiner test negative. The agglutinin did not fix complement and was not eluted from red cell membrane in spite of high temperature.
The findings in this case suggest that either IgM antibody, transformed IgG antibody or IgG isoagglutinin may contribute to the agglutination of red blood cells.抄録全体を表示PDF形式でダウンロード (1470K) -
松井 律東, 佐藤 博紀, 庄 貞行, 上條 一也, 細谷 純一郎, 千住 紀, 尤 芳上, 高木 康, 石井 暢, 奥山 清一, 志村 豁 ...1977 年 37 巻 5 号 p. 505-509
発行日: 1977/10/28
公開日: 2010/09/09
ジャーナル フリーPDF形式でダウンロード (582K)
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