We investigated the effects of NKK-105 and its related drugs (Diisopropyl 4-hydroxy-1, 3-dithiolan 2-ylidene malonate: NKK-103, Diisopropyl 1, 3-dithiolan 2-ylidene malonate: NKK-100) on the bile-expelling mechanism in the isolated gall bladder, the isolated Oddi-sphincter and the isolated duodenum of guinea-pig and rabbit. The results were as follows: 1) NKK-105 and its related drugs inhibited the spontaneous movement of Oddi-sphincter with a dose of over 1×10-6g/ml. 2) NKK-105 and its related drugs caused the inhibition of spontaneous movement and the relaxation and lowering of internal pressure in gall bladder of guinea-pig. 3) The pretreatment of NKK-105 and its related drugs slightly inhibited the acetylcholine (Ach) -induced contraction. The inhibitory action of NKK-105 and its related drugs on the BaCl2 or Ach-induced contraction was more potent in the BaCl2 than in the Ach. From these results, it suggested that relaxation by NKK-105 and its related drugs directly acted on the biliary system. 4) The inhibition of spontaneous movement and the relaxation of smooth muscles on the isolated biliary system increased in the order of NKK-100 <NKK-103 <NKK-105. 5) It seemed that relaxation of NKK-105 and its related drugs on the Oddi-sphincter accelerated to the expelling of bile into the duodenum.
We investigated the effects of combined administration or after treatment of NKK-105 and its related compounds on the CCl4-induced fatty liver in the rats, and obtained the following results. 1) The liver per body weight (L/B) ratio increased by the administration of CCl4 (0.5ml/kg, s. c. daily for 4 days) . An increase of L/B ratio by CCl4 inhibited by the administration of NKK-105 and its related compounds with a dose of 10-50 mg/kg, p, o.. But NKK-105 and its related compounds 250 mg/kg, p. o. administered group showed an increase of L/B ratio more than CCl4 administered group. 2) CCl4 administered group showed a decrease in protein of liver and serum. NKK-105 and its related compounds inhibited the decrease of protein by the administration of CCl4, especially in NKK-150 250 mg/kg, p. o. administered group and it was showed an increase of protein more than control group. 3) The liver lipids of CCl4-induced fatty liver showed a significantly increase in total lipids (TL), total cholesterols (TC) and triglyceride (TG) and a slightly decrease in total phospholipids (PL) . NKK-105 and its related compounds inhibited the increase of TL, TC and TG and decrease of PL by the administration of CCl4. NKK-105 250 mg/kg, p. o. administered group showed an increase of PL more than control group. 4) The serum lipids of CCl4-induced fatty liver showed a decrease of TL, TC, TG and PL. NKK-105 and its related compounds inhibited the decrease of serum lipids by the administration of CCl4. NKK-105 250 mg/kg, p. o. administered group showed an increase of over control group. 5) The rise of serum transaminase was observed by the administration of CCl4. This transaminase rising action of CCl4 was inhibited by the administration of NKK-105 and its related compounds. 6) Histologic examination of CCl4-induced fatty liver showed a centrilobular fatty degeneration, necrosis and loss of nucleus. NKK-105 and its related compounds showed an inhibitory effect of these histological response to CCl4 administration, especially in 250 mg/kg, p. o. of NKK-105 markedly.
To asses the age-related change of growth hormone (GH) secretion in man, blood GH levels on insulin tolerance test, arginine infusion test and blood sumples on early sleeping time were assayed in normal elderly subjects (over aged 60) and young subjects (aged 15 to 35) . The fasting and maximal levels in both insulin and arginine tests were not significantly altered with aging. To insulin tolerance test, the mean maximal level of whole subjects was on 60 minuites, but 2 of 8 young subjects reached on 30 minuites, while 1 of 8 elderly subjects did on 120 minuites. On time of decreasing phase of blood GH response, the mean GH levels of elderly subjects remained markedly higher than those of young subjects on 120 and 180 minuites after insulin puls and on 120 minuites after arginine infusion. These prolongation in elderly subjects of reaching to maximal value and returnning to basal value were suspected one of specific reactions of the elderly tested their hormonal function. Measurring sleep-related GH secretion, 4 of 9 young subjects showed higher blood GH levels than 5 ng/ml which proposed upper limitt of normal basal value, while all of 10 elderly subjects remained low level under 5 ng/ml. Young and elderly corticosteroid-treated patients whose total dosages of corticosteroids under 5000 mg (prednisolone base) were respected to keep normal GH function by measurring maximal levels on insulin tolerance tests. But the patients whose total dosages over 5000 mg declined GH function, especialy elderly patients suposed to be markedly affected reflecting to their mean maximal level of 8.8±3.0 ng/ml which was less than lower limitt of normal maximal GH value (10 ng/ml) by insulin tolerance test.
In this paper, we report about the keratinase of Microsporum canis. The isolation and purification method was as follows: at first culture broth was filtrated, and then passed through a DEAE Cellulose. The effluent adjusted to pH 6.1, was fixed with a CM Cellulose, eluted with 0.1 M NaCl, and passed again through a DEAE Cellulose. Finally the material was applied to Sephadex G 75. The sample was divided into two Isozyme. The molecular weight of keratinase is about 32, 000 and 20, 000. The keratinase A and B have a pH optimum of 7.5, their optimum temperature is at 47°C, optimum ionic strength is at 0.01 M NaCl in 28 mM phosphate buffer, pH 7.8. Disc electrophoresis was carried out according to the method of Yutaka Nagai. The keratinase A and B each gave a single band on 10% gel run at pH 4.0. These result is differed from the keratinase of Microsporum gypseum and Trichophyton mentagrophytes.
A case, 35 year-old male, of pulmonary embolism was reported, who was admitted with suddenl right chest pain and dyspnea. He was diagnosed by hyperlucency in right upper lobe on chest X-P and perfusion defect of same part with pulomonary perfusion scanning. He was remarkably improved by anticoagulated and fibrinolytic therpies.
A 52--year-old woman underwent operation for the removal of the cerebellar hemisphere cyst under general anesthesia with modified neuroleptanesthesia. Immediately after the absorption of tumor contents, blood pressure fell down. We succeeded the elevation of blood pressure with ephedrine and norepinephrine. Some causes of this hypotension were suggested, but we were not convinced what was the cause. So that we emphasize the importance of monitoring, for example ECG, continuous intraarterial blood pressure recording, intermittent arterial blood gases analysis, continuous auscultation of the heart sounds by esophageal stethoscope, Doppler ultrasonic detector and so forth.
A case of Marfan's syndrome complicated annulo-aortic ectasia is reported. Her brother also had aunulo-aortic ectasia. Moreover, many heart diseases were found in members of the family. Marf an's syndrome is often complicated with severe cardiovascular diseases, however, the report of a pedigree with cardiovascular diseases in Marf an's syndrome is few.
In scintigraphies of forty patients with hemiplegia following appoplexia, focal abnormal accumulations in the chest region were seen in bone scintigraphies of four patients. These hot accumulations could be neither rib fracture, rib metastases, nor abnormal calcium accumulation. A mechanism of these accumulation remains to undicided. We believe that this phenomena is related to abnormal bone metabolism in hemiplegial condition.
Esophageal carcinoma treated at this surgical unit for past 11 years accounted for 103 cases, 90 males and 13 females at the age of 35 to 82, a mean age of 63.0 years. Fourtytwo patients (40.7%) of these received a radical operation. Twelve percent of these patients died following the surgical procedure, and postoperative complications developed in 4.7%, of which 19.0% were pulmonary complications. Pulmonary complications were particularly many in aged patients, 65 years old or over. There were noted no specific correlation between the preoperative respiratory function and the postoperative pulmonary complication. The artificial respiration via a nasotracheal tube immediately after the operation was effective and was thought to be an appropriate procedure to be employed even for aged patients irrespective of absence of preoperative respiratory dysfunction. Swan Ganz catheter is an effective tool for the postoperative management of patients as it allows to seize and deal with, in a relative early postoperative stage, the critical condition of the cardio-pulmonary function.