昭和医学会雑誌
Online ISSN : 2185-0976
Print ISSN : 0037-4342
ISSN-L : 0037-4342
39 巻 , 1 号
選択された号の論文の12件中1~12を表示しています
  • 安原 一, 坂本 浩二
    1979 年 39 巻 1 号 p. 1-6
    発行日: 1979/02/28
    公開日: 2010/09/09
    ジャーナル フリー
  • 栗本 忠, 中山 貞男, 西村 忠典, 和田 育穂, 坂本 浩二
    1979 年 39 巻 1 号 p. 7-17
    発行日: 1979/02/28
    公開日: 2010/09/09
    ジャーナル フリー
    We investigated the effects of NKK-105 and its related drugs (Diisopropyl 4-hydroxy-1, 3-dithiolan 2-ylidene malonate: NKK-103, Diisopropyl 1, 3-dithiolan 2-ylidene malonate: NKK-100) on the bile-expelling mechanism in the isolated gall bladder, the isolated Oddi-sphincter and the isolated duodenum of guinea-pig and rabbit.
    The results were as follows:
    1) NKK-105 and its related drugs inhibited the spontaneous movement of Oddi-sphincter with a dose of over 1×10-6g/ml.
    2) NKK-105 and its related drugs caused the inhibition of spontaneous movement and the relaxation and lowering of internal pressure in gall bladder of guinea-pig.
    3) The pretreatment of NKK-105 and its related drugs slightly inhibited the acetylcholine (Ach) -induced contraction.
    The inhibitory action of NKK-105 and its related drugs on the BaCl2 or Ach-induced contraction was more potent in the BaCl2 than in the Ach.
    From these results, it suggested that relaxation by NKK-105 and its related drugs directly acted on the biliary system.
    4) The inhibition of spontaneous movement and the relaxation of smooth muscles on the isolated biliary system increased in the order of NKK-100 <NKK-103 <NKK-105.
    5) It seemed that relaxation of NKK-105 and its related drugs on the Oddi-sphincter accelerated to the expelling of bile into the duodenum.
  • 中山 貞男, 岡崎 雅子, 酒井 節代, 中村 幸雄, 坂本 浩二
    1979 年 39 巻 1 号 p. 19-28
    発行日: 1979/02/28
    公開日: 2010/09/09
    ジャーナル フリー
    We investigated the effects of combined administration or after treatment of NKK-105 and its related compounds on the CCl4-induced fatty liver in the rats, and obtained the following results.
    1) The liver per body weight (L/B) ratio increased by the administration of CCl4 (0.5ml/kg, s. c. daily for 4 days) .
    An increase of L/B ratio by CCl4 inhibited by the administration of NKK-105 and its related compounds with a dose of 10-50 mg/kg, p, o.. But NKK-105 and its related compounds 250 mg/kg, p. o. administered group showed an increase of L/B ratio more than CCl4 administered group.
    2) CCl4 administered group showed a decrease in protein of liver and serum.
    NKK-105 and its related compounds inhibited the decrease of protein by the administration of CCl4, especially in NKK-150 250 mg/kg, p. o. administered group and it was showed an increase of protein more than control group.
    3) The liver lipids of CCl4-induced fatty liver showed a significantly increase in total lipids (TL), total cholesterols (TC) and triglyceride (TG) and a slightly decrease in total phospholipids (PL) .
    NKK-105 and its related compounds inhibited the increase of TL, TC and TG and decrease of PL by the administration of CCl4. NKK-105 250 mg/kg, p. o. administered group showed an increase of PL more than control group.
    4) The serum lipids of CCl4-induced fatty liver showed a decrease of TL, TC, TG and PL.
    NKK-105 and its related compounds inhibited the decrease of serum lipids by the administration of CCl4. NKK-105 250 mg/kg, p. o. administered group showed an increase of over control group.
    5) The rise of serum transaminase was observed by the administration of CCl4.
    This transaminase rising action of CCl4 was inhibited by the administration of NKK-105 and its related compounds.
    6) Histologic examination of CCl4-induced fatty liver showed a centrilobular fatty degeneration, necrosis and loss of nucleus.
    NKK-105 and its related compounds showed an inhibitory effect of these histological response to CCl4 administration, especially in 250 mg/kg, p. o. of NKK-105 markedly.
  • 黒沢 安彦, 安原 一, 小口 勝司, 小林 真一, 小川 道子, 庄 貞行
    1979 年 39 巻 1 号 p. 29-38
    発行日: 1979/02/28
    公開日: 2010/09/09
    ジャーナル フリー
    牛肝臓mitochondria MAOに対する種々glycol類の影響を検討した.MAO活性はWarburg検圧計を使用し, 反応開始後1時間における酸素消費量を測定して決定した.ethylene glycolはその10%の濃度で約35%の, 20%の濃度で約40%の, 30%の濃度で約35%のそれぞれMAO活性増大を示した.しかし, polyethylene glycolはその0.1%から20%の濃度においてMAO活性になんら影響を与えなかつた.一方propylene glycolは10%の濃度で約20%の, 20%の濃度で約30%のそれぞれMAO阻害作用を示した.酵素材料として牛肝臓homogenateを使用した場合でも, またmitochondriaから可溶化したものを使用した場合でもethylene glycolのMAO賦活作用は同じように認められた.またこの賦活作用はtyramine, butylamine, β-phenylethylamine, benzylamine, amylamine, tryptamine, serotoninのいずれの基質を用いた場合でも同様に認められた.このethylene glycolを一度酵素液と混和したのち一夜透析するとMAO活性は完全にもとに復し, この賦活作用は明らかに可逆的な作用であることが判明した.tyramineを基質としたときの牛肝臓mitochondria MAOのKmは2×10-2Mにあつた.これに10%のethylene glycolを添加するとKmは変化しなかつたがVmaxが約2倍に増加した.さらにこのethylene glycol添加により牛肝臓mitochondria MAOの至適pHは7.0から7.5に移動し, また10-1.5M以上の高濃度の基質のもとではこのethylene glycol添加により過剰基質阻害が認められるようになつた.以上の結果から, ethylene glycolはMAOの反応において酵素と基質との結合後の次の段階を促進することによつてこの反応を賦活しているものと考えられる.
  • 杉田 玄
    1979 年 39 巻 1 号 p. 39-48
    発行日: 1979/02/28
    公開日: 2010/09/09
    ジャーナル フリー
    To asses the age-related change of growth hormone (GH) secretion in man, blood GH levels on insulin tolerance test, arginine infusion test and blood sumples on early sleeping time were assayed in normal elderly subjects (over aged 60) and young subjects (aged 15 to 35) .
    The fasting and maximal levels in both insulin and arginine tests were not significantly altered with aging. To insulin tolerance test, the mean maximal level of whole subjects was on 60 minuites, but 2 of 8 young subjects reached on 30 minuites, while 1 of 8 elderly subjects did on 120 minuites.
    On time of decreasing phase of blood GH response, the mean GH levels of elderly subjects remained markedly higher than those of young subjects on 120 and 180 minuites after insulin puls and on 120 minuites after arginine infusion. These prolongation in elderly subjects of reaching to maximal value and returnning to basal value were suspected one of specific reactions of the elderly tested their hormonal function.
    Measurring sleep-related GH secretion, 4 of 9 young subjects showed higher blood GH levels than 5 ng/ml which proposed upper limitt of normal basal value, while all of 10 elderly subjects remained low level under 5 ng/ml.
    Young and elderly corticosteroid-treated patients whose total dosages of corticosteroids under 5000 mg (prednisolone base) were respected to keep normal GH function by measurring maximal levels on insulin tolerance tests. But the patients whose total dosages over 5000 mg declined GH function, especialy elderly patients suposed to be markedly affected reflecting to their mean maximal level of 8.8±3.0 ng/ml which was less than lower limitt of normal maximal GH value (10 ng/ml) by insulin tolerance test.
  • 清 佳浩, 樋口 道生, 滝内 石夫
    1979 年 39 巻 1 号 p. 49-54
    発行日: 1979/02/28
    公開日: 2010/09/09
    ジャーナル フリー
    In this paper, we report about the keratinase of Microsporum canis. The isolation and purification method was as follows: at first culture broth was filtrated, and then passed through a DEAE Cellulose. The effluent adjusted to pH 6.1, was fixed with a CM Cellulose, eluted with 0.1 M NaCl, and passed again through a DEAE Cellulose. Finally the material was applied to Sephadex G 75. The sample was divided into two Isozyme. The molecular weight of keratinase is about 32, 000 and 20, 000. The keratinase A and B have a pH optimum of 7.5, their optimum temperature is at 47°C, optimum ionic strength is at 0.01 M NaCl in 28 mM phosphate buffer, pH 7.8. Disc electrophoresis was carried out according to the method of Yutaka Nagai. The keratinase A and B each gave a single band on 10% gel run at pH 4.0. These result is differed from the keratinase of Microsporum gypseum and Trichophyton mentagrophytes.
  • 小川 道子
    1979 年 39 巻 1 号 p. 55-63
    発行日: 1979/02/28
    公開日: 2010/09/09
    ジャーナル フリー
    家兎肝homogenateを酵素材料とし, これに対する保存, 熱処理, 薬物処理のMAO活性に対する影響を, 各種基質, 阻害剤を使用しMAOによる酸素消費量を測定して検討した.基質としてはtyramine, benzylamine, serotonin, β-phenylethylamine, tryptamineを用い, 阻害剤としてはpargyline, harmaline, tranylcypromineを使用し反応中に消費される酸素量の変化をWarburg検圧計により測定した.新鮮酵素および冷凍保存した場合の酵素の活性を経時的に比較した結果, 前者の場合tyramine, serotonin, tryptamineを基質とした際のMAO活性は直線的に増大したが, benzylamineまたはβ-phenylethylamineでは反応開始30分経過後に活性の減弱がみられた.一方, 冷凍保存酵素ではいずれの基質の場合も活性は低く, 両者では相違がみられた.本臓器中MAOの複数性を検討するため基質tyramineまたはbenzylamineを使用して, 新鮮酵素と保存処理した酵素における活性の経時的変化および各阻害剤による影響を検討し以下の結果を得た.
    1) 家兎肝homogenateを室温 (22℃) に放置しtyramineまたはbenzylamineを基質としてその安定性を検討した結果, 放置後24時間まではMAO活性は変化せず, 26時間後では両基質ともその活性は約50%失活し, 29時間後ではほぼ完全に消失した.
    2) 冷蔵庫 (4℃) に保存した場合, 日数の経過とともにMAO活性は低下したが, benzylamineを基質とした場合の活性の低下はtyramineを基質とした場合のそれよりも著明であつた.
    3) さらに冷凍庫 (-20℃) に保存した場合, 4週間まではMAO活性に変化は認められなかつたが, その後その活性は急激に低下し, 12週間後のMAO活性の減少率は基質tyramineの場合25%, benzylamineでは約51%であつた.
    4) 新鮮な酵素材料と, 1週間冷蔵庫に保存したhomogenateのMAOに対するharmaline, tranylcypromine, pargylineの影響を検討した.阻害剤としてtranylcypromineおよびpargylineを用いた場合, 酵素の保存条件間ないし両基質問におけるMAO活性の阻害作用には差は認められなかつた.Harmalineを阻害剤として新鮮酵素材料を使用した場合, tyramineを基質とした方がbenzylamineを基質とした場合よりも強いMAO阻害作用を示したが, 保存日数の経過した酵素材料では両基質におけるMAO活性阻害度には相違は認められなくなつた.
    5) 家兎肝MAOは熱処理に対して, 基質benzylamineの場合よりも, tyramineを基質とした場合の方が安定であつた.
    6) 家兎肝MAO活性はtrypsin処理によつて著明に減少した.しかし両基質におけるMAO活性の阻害度に相違は認められなかつた.
    以上の結果から, 家兎肝homogenate中には酵素化学的に性質の異なる複数のMAOが存在するものと考えられる
  • 中神 和清, 井関 隆, 西山 博明, 綱川 宏, 木村 肇, 近藤 攻三, 国枝 武文, 鈴木 一, 里見 智正, 猿田 栄助, 野口 英 ...
    1979 年 39 巻 1 号 p. 65-69
    発行日: 1979/02/28
    公開日: 2010/09/09
    ジャーナル フリー
    A case, 35 year-old male, of pulmonary embolism was reported, who was admitted with suddenl right chest pain and dyspnea. He was diagnosed by hyperlucency in right upper lobe on chest X-P and perfusion defect of same part with pulomonary perfusion scanning. He was remarkably improved by anticoagulated and fibrinolytic therpies.
  • 庭野 信子, 安本 和正, 松崎 史朗, 細山田 明義
    1979 年 39 巻 1 号 p. 71-74
    発行日: 1979/02/28
    公開日: 2010/09/09
    ジャーナル フリー
    A 52--year-old woman underwent operation for the removal of the cerebellar hemisphere cyst under general anesthesia with modified neuroleptanesthesia. Immediately after the absorption of tumor contents, blood pressure fell down. We succeeded the elevation of blood pressure with ephedrine and norepinephrine. Some causes of this hypotension were suggested, but we were not convinced what was the cause. So that we emphasize the importance of monitoring, for example ECG, continuous intraarterial blood pressure recording, intermittent arterial blood gases analysis, continuous auscultation of the heart sounds by esophageal stethoscope, Doppler ultrasonic detector and so forth.
  • 松本 一夫, 小林 洋一, 長谷川 貢, 小林 正樹, 藤田 良範, 吉田 文英, 新谷 博一
    1979 年 39 巻 1 号 p. 75-80
    発行日: 1979/02/28
    公開日: 2010/09/09
    ジャーナル フリー
    A case of Marfan's syndrome complicated annulo-aortic ectasia is reported. Her brother also had aunulo-aortic ectasia. Moreover, many heart diseases were found in members of the family. Marf an's syndrome is often complicated with severe cardiovascular diseases, however, the report of a pedigree with cardiovascular diseases in Marf an's syndrome is few.
  • 丸山 俊章, 竹内 方志, 徳永 宏司, 前田 陽一, 長谷川 一彦, 北原 隆, 菱田 豊彦
    1979 年 39 巻 1 号 p. 81-86
    発行日: 1979/02/28
    公開日: 2010/09/09
    ジャーナル フリー
    In scintigraphies of forty patients with hemiplegia following appoplexia, focal abnormal accumulations in the chest region were seen in bone scintigraphies of four patients.
    These hot accumulations could be neither rib fracture, rib metastases, nor abnormal calcium accumulation.
    A mechanism of these accumulation remains to undicided. We believe that this phenomena is related to abnormal bone metabolism in hemiplegial condition.
  • 舟木 正朋, 高場 利博, 松田 賢, 石井 淳一
    1979 年 39 巻 1 号 p. 87-92
    発行日: 1979/02/28
    公開日: 2010/09/09
    ジャーナル フリー
    Esophageal carcinoma treated at this surgical unit for past 11 years accounted for 103 cases, 90 males and 13 females at the age of 35 to 82, a mean age of 63.0 years. Fourtytwo patients (40.7%) of these received a radical operation. Twelve percent of these patients died following the surgical procedure, and postoperative complications developed in 4.7%, of which 19.0% were pulmonary complications. Pulmonary complications were particularly many in aged patients, 65 years old or over. There were noted no specific correlation between the preoperative respiratory function and the postoperative pulmonary complication. The artificial respiration via a nasotracheal tube immediately after the operation was effective and was thought to be an appropriate procedure to be employed even for aged patients irrespective of absence of preoperative respiratory dysfunction. Swan Ganz catheter is an effective tool for the postoperative management of patients as it allows to seize and deal with, in a relative early postoperative stage, the critical condition of the cardio-pulmonary function.
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