生体医工学
Online ISSN : 1881-4379
Print ISSN : 1347-443X
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54Annual 巻 , 27AM-Abstract 号
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  • 髙谷 節雄
    54Annual 巻 (2016) 27AM-Abstract 号 p. S132
    公開日: 2016/11/19
    ジャーナル フリー
  • 鈴木 亮, 小田 雄介, ウンガ ヨハン, 小俣 大樹, 根岸 洋一, 丸山 一雄
    54Annual 巻 (2016) 27AM-Abstract 号 p. S133
    公開日: 2016/11/19
    ジャーナル フリー

    Ultrasound is a useful tool for diagnostics and therapeutics in the medical field, and microbubbles can be used to improve diagnostics with ultrasonography. Recently, it was reported that the combination of nano- and micro-sized bubbles and ultrasound can enhance the effects of diagnostics and therapeutics. Therefore, this combination could be a new technology for ultrasound theranostics combined therapy and diagnostics. Especially, this theranostics is showing promise as drug delivery system and for tumor treatment. In this presentation, we will introduce our bubble preparation and feasibility studies of our bubbles for ultrasound theranostics.

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  • 梅田 泉
    54Annual 巻 (2016) 27AM-Abstract 号 p. S134
    公開日: 2016/11/19
    ジャーナル フリー

    In cancer diagnosis and therapy, radionuclide imaging and radionuclide therapy by using their radiation energy are informative and promising. Large tumor accumulation is needed for their success. Liposomes, one of the nanoparticles of drug delivery system, have a potential to deliver drugs to the tumor via the enhanced permeability and retention (EPR) effect. We have been studying application of radionuclide-encapsulated liposomes for tumor imaging and therapy. It is also attractive as "theranostic" agent, because both radionuclides for imaging and therapy can be encapsulated in the same liposomes. However, conventional radionuclide-encapsulated liposomes also accumulate in the normal tissues, such as the liver and spleen, hindering their clinical application. I herein present a new strategy focusing on the radionuclide-ligand complexes inside the liposomes that is able to escape from the liver after the accumulation and be rapidly excreted into urine.

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  • 西山 雅祥
    54Annual 巻 (2016) 27AM-Abstract 号 p. S135
    公開日: 2016/11/19
    ジャーナル フリー

    Water is the most abundant substance in cells. Most intracellular reactions occur with the association of water molecules that surround the protein molecules. Application of pressure is a powerful method for modulating intermolecular interactions between protein and water molecules. This means that applied pressure is able to modulate the structure and function of protein molecules, without requiring the use of any chemical materials other than water molecules. We have developed a microscope that enables us to acquire high-resolution microscopic images at high-pressure conditions. The developed system allows us to visualize and manipulate the cellular architecture and activity.

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  • 花岡 健二郎
    54Annual 巻 (2016) 27AM-Abstract 号 p. S136
    公開日: 2016/11/19
    ジャーナル フリー

    Fluorescence imaging is one of the most powerful techniques for visualization of the temporal and spatial biological events in living cells, and fluorescent probes are also highly useful for fluorescence imaging. The light in near-infrared (NIR) region is recently paid much attention because of its high tissue penetration and low autofluorescence. In this presentation, we will report the development of small-molecule NIR fluorescent probes for hypoxia (Angew. Chem. Int. Ed., 52, 13028-13032 (2013)) and a small-molecule MRI-fluorescence dual imaging probe for atherosclerosis that specifically targets the atherosclerotic environment (Org. Biomol. Chem., 12, 8611-8618 (2014)).

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  • 山脇 成人
    54Annual 巻 (2016) 27AM-Abstract 号 p. S137
    公開日: 2016/11/19
    ジャーナル フリー

    急増するうつ病は休職や自殺の要因ともなっており、その対策は国家的課題となっている。日本医療研究開発機構(AMED)の脳科学研究戦略推進プログラム(脳プロ)において、うつ病の病態に基づいた客観的診断法と革新的治療法の開発が重要なテーマとなっている。うつ病の病態は多様であるが、現在のうつ病診断は、抑うつ気分などの症候に基づいており病態に基づいていない。治療も試行錯誤で行われているのが実情で、適切な診断・治療のためには、うつ病の神経回路・分子病態に基づく診断と治療法開発が必要不可欠である。

     うつ病発症には、遺伝的要因と養育環境など相互作用による神経回路形成不全とモノアミン、グルタミン酸異常などの分子病態が関与するが、その神経回路異常と分子病態の関連はまだ不明な点が多い。本講演では、うつ病診療の現状と課題に加えて、演者らが脳プロで行ってきた脳由来神経栄養因子(BDNF)に関する分子病態研究、機能的MRIを用いた脳機能画像解析によるうつ病症候に関連する神経回路異常、これらの多次元データを機械学習を用いたうつ病の客観的診断法、治療反応性予測法の開発などの研究成果を中心に、最新のうつ病の脳科学研究を紹介する。最後にうつ病のニューロフィードバック治療法開発を含めうつ病の革新的治療法の今後の展望についてものべる。

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  • 大橋 三男
    54Annual 巻 (2016) 27AM-Abstract 号 p. S138
    公開日: 2016/11/19
    ジャーナル フリー

    fNIRS(functional near-infrared spectroscopy equipment)が、平成26年4月より「D236-2光トポグラフィー / 抑うつ症状の鑑別診断の補助に使用するもの」検査で医科診療報酬点数が与えられた。すなわち保険適用となった。fNIRSは1990年代から始まって脳科学研究の世界では広く使われ実績を上げてきたが、いよいよ臨床現場での使用が開始された。従来型の医療機器であるCTとかMRIといった生体の形状検査ではなく、脳機能検査が保険適用になったのは画期的である。

     このfNIRSでは多点における脳血流変化を同時計測している(保険適用では最低10チャネルが要求されている)。近赤外線光を外部から生体に出射し、脳内部で吸収と散乱を受け生体外部に戻ってきた微弱光を高感度光センサーで捉えて計測する技術である。このfNIRSの基盤となる技術として光変調技術がある。従来はTDMA(時分割多重)あるいはFDMA(周波数分割多重)という変調方法が使われていた。我々が開発した最新技術のCDMA(符号分割多重)を使ったfNIRS(光イメージング脳機能測定装置Spectratech OEG-16ME)も昨年4月に保険適用に認められた。CDMAの最大のメリットは高SNR(信号対雑音比)であること、変復調回路規模は極めてシンプルで小さく、機器の小型化に大きく貢献していることである。今回、その概要を紹介する。

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  • 大橋 由明
    54Annual 巻 (2016) 27AM-Abstract 号 p. S139
    公開日: 2016/11/19
    ジャーナル フリー

    大うつ病性障害(MDD)の罹患率は全世界で4.37%であるが、わが国では2.46%と言われており、韓国と並んで「うつ病患者の少ない国」である(Ferrari et al, PLoS Med., 10:e1001547, 2013)。これは、うつ病という疾病が適切に診断されていない、もしくは患者を発見できていないことに起因すると考えている。MDDを血液による診断方法の開発は、このような事態を改善するために有効であろう。そこで、自社の持つメタボローム解析技術により、MDD患者を鑑別できる代謝物質バイオマーカー(mBM)を探索した。解析の結果、リン酸エタノールアミン(PEA)が有望なmBM候補となり、確認試験などを経て実証を行った。また、検査キットの開発にも着手している。本講演では、PEA発見から検査キットの開発までを紹介する。

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  • 岸本 泰士郎
    54Annual 巻 (2016) 27AM-Abstract 号 p. S140
    公開日: 2016/11/19
    ジャーナル フリー

    精神疾患におけるバイオマーカーの不足は、治療や研究の大きな障壁となっている。現在、精神疾患の重症度は、多くが評価者との面接を通じた本人の自覚症状の聴取や他者による観察によって評価されるレーティングスケールを用いて行われている。しかし、自覚症状や他者の観察は客観性に乏しく再現性が低い事、レーティングスケールの問診に時間がかかり過ぎる事などから、日常臨床ではなかなか用いられず、治験では新薬の効果が検出しづらいなどの問題が生じている。

     そこで我々は精神科医が日常臨床で重症度の指標としている精神運動抑制や気分の表出を、機械学習を用いて客観的に定量化するというプロジェクトを開始した。具体的には患者の表情、音声、体動データを、機械学習を用いたアプローチで定量化し、ゴールドスタンダードとして用いられている評価尺度に高い相関が得られるようなアルゴリズムを開発するというものである。発表では、海外における類似の研究の紹介とともに、この技術開発における背景、課題などについて述べる。

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  • 山本 義春, 中村 亨
    54Annual 巻 (2016) 27AM-Abstract 号 p. S141
    公開日: 2016/11/19
    ジャーナル フリー

    The impact of development of ICT to medical and healthcare services has recently been discussed. In particular, the prevalence of intelligent wearable devices and embedded sensors in daily living environment enable to measure high-quality, multidimensional, and intensive longitudinal biomedical data (ILD) and also access large-scale health-related information. The utility of such data is thought to have a great potential to diagnosis and an early detection of diseases. Therefore, the establishment of a new framework to utilize it, especially a method to extract the useful information associated with diseases, has been focused. Indeed, Horizon2020 in EU incorporated such viewpoints into the projects called in the field of medicine and healthcare. Based on these situations, JST-CRDS also reported on the establishment of proactive health and medical systems for the control of health-related risks. In this talk, we briefly introduce this report and a new data mining framework for biomedical ILD.

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  • 合原 一幸
    54Annual 巻 (2016) 27AM-Abstract 号 p. S142
    公開日: 2016/11/19
    ジャーナル フリー

    In this talk, I will review bases of theory of DNB (Dynamical Network Biomarkers) analysis and its application to detection of predisease states. The DNB analysis is based on bifurcation theory for a complex biological network that triggers a disease, and can detect a predisease state bifurcating from a healthy-state attractor to a disease-state attractor. The DNB analysis can realize very early personalized diagnosis and preemptive medicine for various complex diseases.

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  • 中村 亨
    54Annual 巻 (2016) 27AM-Abstract 号 p. S143
    公開日: 2016/11/19
    ジャーナル フリー

    We found the robust statistical laws of behavioral organization, specifically the laws of durations of resting and active periods in spontaneous physical activity (SPA), as well as their alterations in psychiatric disorders, including major depressive disorder. These strongly suggest the existence of the dynamical system organizing SPA and its dysfunctions in the disorders. In this talk, we introduce our recent studies on the reconstruction of the dynamical systems based on SPA in patients with psychiatric disorders, showing the alterations of their dynamical properties in the disorders. Further, using the intensive longitudinal data of SPA in patients with bipolar disorder, we demonstrate the concurrent changes of the reconstructed systems, associated with pathological states or clinical phases, along with the nontrivial changes around the vicinity of clinical phase transitions. These could lead to the objective monitoring of pathological states of psychiatric disorders and an early warning detection of pathological changes.

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  • 清野 健
    54Annual 巻 (2016) 27AM-Abstract 号 p. S144
    公開日: 2016/11/19
    ジャーナル フリー

    Normal heart contraction is initiated by electrical impulses from the sinoatrial (SA) node acting as the natural pacemaker of the heart. The heartbeat intervals fluctuate in a complex manner even for a healthy individual at rest. This fluctuation is caused by that of the pacing rate of the SA node modulated by the activity of sympathetic and/or parasympathetic nervous fibers via the autonomic nervous system. Therefore, through the analysis of heart rate fluctuations, called heart rate variability (HRV), it is possible to evaluate various aspects of ANS function. It has been reported that reduced and/or abnormal HRV in cardiac patients is associated with higher mortality risk. Hence, HRV are expected to serve as prognostic as well as diagnostic markers of various cardiovascular disorders. In this presentaion, we discuss characteristics of HRV and the key issues for evaluation of ANS functions thought to play an important role in the disease transition.

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  • 鈴木 康之, 野村 泰伸
    54Annual 巻 (2016) 27AM-Abstract 号 p. S145
    公開日: 2016/11/19
    ジャーナル フリー

    ヒト静止立位時の姿勢動揺は,足関節をはじめとする複数関節の協調的な変動によって生じる.一般的な動的システムの安定性に関する直感に基づけば,「姿勢動揺が大きいほど姿勢の安定性は低い」と考えられる.ところが,姿勢反射障害のあるパーキンソン病患者の姿勢動揺のサイズは,健常者に比べて必ずしも大きいわけではなく,直感的な安定性の定量化が常に正しいとは限らない.我々は,健常なヒト立位姿勢の神経制御機構として間欠制御仮説を提唱している.これは「中枢神経系は,能動的フィードバック制御を間欠的に不活性化し,能動的制御が作用していないときの姿勢状態空間に存在するサドル型不安定平衡点の安定多様体上の機械的ダイナミクスを間欠的に利用することによって立位姿勢を安定化する」という仮説である.ここでは,静止立位姿勢を倒立二重振子でモデル化し,姿勢動揺における足および股関節の協調運動の動特性が,系の相空間内における安定多様体の幾何学に基づいて理解できることを示す.さらに,パーキンソン病に起因する姿勢動揺の変容を定量化し,それが制御における間欠性の欠如に起因する可能性について議論する.

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  • 川嶋 健嗣
    54Annual 巻 (2016) 27AM-Abstract 号 p. S146
    公開日: 2016/11/19
    ジャーナル フリー

    In this paper, pneumatically-driven surgical assist robots for minimally invasive surgery (MIS) are introduced. The smooth and continuous movement needed for highly precise control of surgical robots have realized by pneumatic servo control. We mainly introduce two robots. One is the endoscope holder for MIS where the camera is held by robot arm that follows the operator's head movement. The other is a novel hand-held robotic forceps for MIS. Intuitive operation is realized by an active transformation from the surgeon's wrist-rotation to the tip-rotation using an inertia measurement unit. The effectiveness of the robots is confirmed with in-vitro and in-vivo experiments.

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  • 高山 俊男, 小俣 透
    54Annual 巻 (2016) 27AM-Abstract 号 p. S147
    公開日: 2016/11/19
    ジャーナル フリー

    If a novel concept machine is developed for many purpose at once, the machine can become overly complicated. Such complicated machine is not suitable for practical use because of its low reliability and low cost performance. Of course, cost performance can often be ignored in surgical robots development. However, expensive robots can be introduced only in large hospitals, which might limit the reach of its benefit to many people. Therefore, we are developing simple mechanisms by determining essential functions for each of the operations.

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  • 多田隈 建二郎, 高根 英里, 田中 信行, 原口 裕次, 長谷川 明之, 清水 達也, 大和 雅之, 岡野 光夫
    54Annual 巻 (2016) 27AM-Abstract 号 p. S148
    公開日: 2016/11/19
    ジャーナル フリー

    従来のチューブ状組織を作製する研究は,中心から棒状構造を取り除くのが非常に難しい状態にあった.このような状況を踏まえ,我々は,従来取り組んできた細胞シート用ヘラ機構を機能発展させて,繰出し型のベルト構造を円形断面状に配置させた構造の研究を行ってきた.細胞シート工学を基盤とした革新的立体臓器製造技術の開発において,脱細胞化した小腸などの管腔状内壁面へ簡易的且つ確実に細胞シートを貼り付けることが可能な装置の実現が求められている.この貼り付け動作を可能とする構造として,先端から折返す円形断面袋構造を有する膨張式貼り付け機構を考案し,簡易実験により,その貼り付け動作が可能であることを確認したので報告する.

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  • 中川 敦寛
    54Annual 巻 (2016) 27AM-Abstract 号 p. S149
    公開日: 2016/11/19
    ジャーナル フリー

    The mission of Japan Biodesign is to train the next generation of entrepreneurs and leaders in biomedical technology innovation for Japan and the global healthcare community.

    The program is launched as a collaboration between Osaka University, Tohoku University, the University of Tokyo, the Japan Federation of Medical Devices Associations (JFMDA) and the Program in Biodesign at Stanford University. Training of the core faculty for Japan Biodesign started since 2014. Leaders from Stanford Biodesign did and will provide extensive mentoring for Japan Biodesign.

    Faculties from these institutions work together to create new, interdisciplinary training structures and methodologies based on the Biodesign Process developed at Stanford. Japan Biodesign involves a novel ”All Japan” government-academia-industry partnership with support from Japan's Ministry of Education.

    The presentation will provide the strategic planning of the program to contribute to creating ecosystem through development of entrepreneurs and innovative medical products and system from Japan.

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  • 前田 祐二郎
    54Annual 巻 (2016) 27AM-Abstract 号 p. S150
    公開日: 2016/11/19
    ジャーナル フリー

    Biodesign process consists from three phases; Identify, Invent & Implement. 7 months has passed since the 2015-2016 Japan Biodesign fellowship started. Currently, fellows are working on Invent and Implement phases. As Dr. Tomas J. Fogarty said in Biodesign textbook, “An idea, by itself, has no importance whatsoever; it is the implementation that idea and its acceptance by others that brings benefits to our patients.”

    The purpose of the Implement phase is to create a multi-year plan for developing concepts into real product that improve patients' lives and is attractive to providers and payers to continue projects after finishing fellowship. We still have much to learn from Silicon Valley about combination of medicine, engineering & business because they have a lot of cases and roll models for young entrepreneurs.

    I believe Japan Biodesign fellows will find ways to implement ideas of young entrepreneurs they identified and invented in Japan with utilizing the resources in Stanford & Silicon valley where medical device startups can develop technologies.

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  • 大森 初夏
    54Annual 巻 (2016) 27AM-Abstract 号 p. S151
    公開日: 2016/11/19
    ジャーナル フリー

    Japan Biodesign Fellowship Program was launched in October 2015, as a hands-on training program to develop talents who will lead medical device innovations in Japan. This 10-month, full-time program consists of a university-based program hosted by three partner universities, Osaka University, The University of Tokyo, and Tohoku University, and a tri-university joint program supported by Stanford University. This year, a total of 10 fellows with diverse background (medicine, engineering and business) joined the program to learn the Biodesign process, which covers need identification in clinical immersion (on-site observation), concept invention, and business planning for implementation. In this presentation, I will report the programs' current progress, including the clinical immersion with university-specific focus, the mentorship provided by Stanford University, and the lectures by real-world experts in specific areas (IP, regulation, reimbursement, and business planning). I will also discuss the remaining curricula such as specialist consultation for concept selection and business development.

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  • 山本 一喜
    54Annual 巻 (2016) 27AM-Abstract 号 p. S152
    公開日: 2016/11/19
    ジャーナル フリー

    Japan Biodesign was launched last October at Osaka University, The University of Tokyo, and Tohoku University in collaboration with Stanford University and JFMDA. Medical device industry is having a high expectation for this program that develops talents who will lead innovation and develop innovative devices based on needs. While the program is developed based on Stanford program, there are different environments between Japan and Stanford. The typical difference is Japan Biodesign plays the role of talent development of working people as well. Therefore we are developing the program which fosters not only entrepreneur but intrapreneur also, and will provide various courses, such as part-time and seminars. Japan Biodesign does not simply implement Stanford program as is. While utilizing the basic concept framework of Stanford program, we will develop a program to foster talents that is suitable for the Japanese environment, in order to respond to the expectation from the industry.

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  • 八木 雅和
    54Annual 巻 (2016) 27AM-Abstract 号 p. S153
    公開日: 2016/11/19
    ジャーナル フリー

    It is estimated that the global market size of ‘Digital Health' will increase drastically to more than $200B, and the market attracts a lot of companies all over the world. In such a setting, “How can Japan win the game by using our advantages?”

    In most false cases of medical device development in Japan, it was found that the companies could not identify the appropriate needs while they had sets of very good high-techs. Namely, finding good needs is the critical factor for success in the development of medical device business, especially in Japan.

    The Stanford Biodesign process, that is the need-pull process for medical device development, can be one of the best solutions to overcome this situation.

    Therefore, we launched Japan Biodesign last year in collaboration with Stanford Biodesign in order to develop the global leaders that can perform the need-pull development process for medtech/healthcare innovation.

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  • 松下 和生, 渡辺 晃也, 横山 萌恵, 石井 豊恵, 木戸 倫子, 山田 憲嗣, 大野 ゆう子, 長倉 俊明
    54Annual 巻 (2016) 27AM-Abstract 号 p. S154
    公開日: 2016/11/19
    ジャーナル フリー

    (Introduction) The biological fluid distribution have been tried to measure by the electrical impedance from 1960. Extracellular fluid including blood and intracellular fluid are consists of electrolytes solution, so these are resistance element. As for the cell wall is made of fat, so it is an insulator by a capacitance. (Purpose) At present the biological fluid measurement is the only method which is large-scale MRI. Therefore, we propose that easy and consecutive measurement method. So we tried whether subcutaneous fluid volume can be measured by bioimpedance. (Result) We found that the impedance from 500kHz to 1MHz is smaller than that of at 100Hz, and impedance is smaller within 1 minute after avascularization. (Conclusion) Because of the brachial cutaneous vein diameter expanded from 0 to 1 minute after avascularization. This phenomenon is approximately agrees with this result. We think that it is possible to measure subcutaneous fluid change by bioimpedance.

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  • 杉本 大己, 日野 遥
    54Annual 巻 (2016) 27AM-Abstract 号 p. S155
    公開日: 2016/11/19
    ジャーナル フリー

    Study of the behavior of the cell by using micropattern is studied now, but it often target one kindof cell. Therefore the comparison of the cell of other kinds is difficult. It is insufficient only by control of the size same as an organization, and the control of the single cell's behavior is necessary for the application to regenerative medicine to make the cellular tissue which is near to the in vivo organization. Three types stripe micropatterns are made to control the extension direction of the cell in one direction by using Photolithography in this study. The manufactured micropatterns has mountain width, 1μm, 3μm, 5μm, height, 1μm, sulcular width, 3μm. The cell's behavior is observed by time lapse photography for 24 hours. About the extension, myoblast and fibroblast were similar results, but neuroblast was different results and confirmed the cell's behavior difference by the size of the groove width.

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  • 石原 瑞希, 野口 展士, 大越 康晴, 荒船 龍彦, 山路 舞香, 鈴木 章泰, 本間 章彦
    54Annual 巻 (2016) 27AM-Abstract 号 p. S156
    公開日: 2016/11/19
    ジャーナル フリー

    Oxygen plasma has been known as enhanced hydrophilic treatment techniques for surface modification.In this study, we investigated effect of oxygen plasma on characterize changes of PLLA fiber sheet and cell adhesion. The PLLA fiber sheet was fabricated by using CO2 gas laser supersonic drawing and the oxygen plasma treatment was performed by r.f. plasma CVD technique. After the plasma treatment, characterized changes of the surface was analyzed by X-ray photoelectron spectroscopy (XPS). The C=O bond ratio of the PLLA fiber sheet surface was increased. Additionally, NIH3T3 cells were spread on the plasma treated PLLA fiber sheet. The cell behavior was analyzed for 50 hours. In an early stage of cell culture, the cell adhesion and proliferation was accelerated by increased C=O bond of the PLLA fiber sheet. It was observed that the oxygen plasma treatment increased C=O bound and had enhancement of cell proliferation.

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  • 日野 遥, 中島 広瑛
    54Annual 巻 (2016) 27AM-Abstract 号 p. S157
    公開日: 2016/11/19
    ジャーナル フリー

    The effect of mechanical stimulation has been studied in vitro. Ultrasonic vibration has clinically applied to regenerative medicine and has treated bone or muscle. However, the effect of ultrasonic vibration on one kind of cell is unclear. In this study, effect of daily ultrasonic vibration (1 MHz, 30 min) on proliferation for some kind of cells was investigated. Cell was cultured on 6 well plate for 4 days. Piezo electric element was placed under well plate and was driven by function generator (16 V pp., sine wave). Vibration was attenuated gradually far from well on piezo electric element. To count number of cell, each of cell suspension absorbance were measured in day 4. Cultured cell in stronger vibrating well was exfoliated from culture surface. Proliferation of C2C12 cell was enhanced with mild vibration, and the optimum vibration depends on the kind of cells.

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  • 中澤 聡, 高木 大輔, 倉持 譲, 松本 貴彦, 増子 龍也, 瀬尾 学, 打方 佳郎, 松崎 典弥, 明石 満
    54Annual 巻 (2016) 27AM-Abstract 号 p. S158
    公開日: 2016/11/19
    ジャーナル フリー

    In vitro development of three-dimensional tissues is in high demand for regenerative medicine and drug screening. Here we present a novel inkjet head specialized for on-demand ejection of cell suspensions. Droplets are formed by membrane vibration, which is generated by a pulse actuation of the piezoelectric vibrator. During the non-ejecting period, the membrane is softly vibrated by a sinusoidal actuation to prevent the sedimentation of the cells inside the chamber.

    We performed an initial feasibility study by ejecting a suspension of normal human dermal fibroblasts (NHDF) using this inkjet head. By combining the ejecting actuation and the stirring actuation, the number of cells per droplets remained stable for more than thirty minutes and cell viability was over 90%.

    Next, we tried to construct three dimensional cardiac tissues by using cardiomyocytes derived from human iPS cells. Droplets with cardiomyocytes were ejected into the culture medium by repeating cycles of ejecting and stirring. After four days of incubation, the cells in the constructed cardiac tissue showed synchronous beating.

    These results indicate that this inkjet head is suitable for the effective ejection of living cells.

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  • 芦葉 裕, 中川 桂一, 塚本 哲, 小林 英津子, 佐久間 一郎
    54Annual 巻 (2016) 27AM-Abstract 号 p. S159
    公開日: 2016/11/19
    ジャーナル フリー

    Various techniques have been developed for studying cell response induced by mechanical stress such as modulating its structure or gene expression. However, conventional techniques and devices cannot load mechanical stress including high-frequency component of kHz to MHz expected to act greatly on cells because of their viscoelastic properties and induce unknown effects. Here we have developed an impulsive stimulation loading system which enables to push the cultured cells with a short duration. Requirements for the system are that loading time of stimulation is 1 μs to 100 μs and displacement imparted to cell is 1 nm to 1 μm. For generating high speed displacement, we drive a cantilever by irradiating nanosecond laser pulse. The displacement was measured by optical lever system with 25 nm and 2 ns resolution. In evaluation experiment, the cantilever was displaced over 1 μm with 4 μs, which meets the requirements.

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  • 三浦 恒, 柳橋 隆全, 馮 忠剛, 小沢田 正, 佐藤 大介, 中村 孝夫, 梅津 光生
    54Annual 巻 (2016) 27AM-Abstract 号 p. S160
    公開日: 2016/11/19
    ジャーナル フリー

    The effects of mechanical properties of the culture substrate on the cellular behavior are intensively investigated. However, most of the studies adjusted the elastic modulus of culture substrates by changing the substrate component concentration. In this study, we take the advantage of the nonlinear mechanical properties of porcine amniotic membrane to realize the sole adjustment of substrate elastic modulus by stretching the membrane at different stresses in a lab-made device. Fibroblasts were able to attach and proliferate nearly to confluence on the membrane (~500 cells/mm2 ) under low stress (elastic modulus smaller than 30.0 kPa); whereas, the cells failed to attach on the membrane under high stress (elastic modulus ~ 6.0 MPa). It implicates that the dynamic characteristics of the amniotic membrane related with the device structure may also impact the cellular behavior because the elastic modulus of conventional culture dishes (~GPa) is much greater than 6.0 MPa.

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  • 外處 侑, アブドゥル ラヒン ヌル シュハダー, 野口 展士, 松永 章弘, 志村 まり, 荒船 龍彦, 本間 章彦
    54Annual 巻 (2016) 27AM-Abstract 号 p. S161
    公開日: 2016/11/19
    ジャーナル フリー

    The three-dimensional cell culture is the powerful tool for evaluate behavior of virus and medicine in vitro compared with two-dimensional dishes. In study we developed two lines perfusion culture systems with three-dimensional culture vessel in which one line was for culture cell and another one was for delivering virus or medicine. The three-dimensional culture vessel is composed of three-dimensional fibrous scaffold which is manufactured by laminating microfibers. This system constructed with closed circuit, silicone tube, roller pump, reservoir and three-dimensional scaffold. All devices except pump were set on CO2 incubator. For evaluating culture condition, we measured continuously pH and temperature change in 96 hours run. As a result, two system perfusion culture systems were able to maintain environment suitable for a cell culture at temperature and pH in 96 hours run.

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  • 木村 剛, 呉 平麗, 中村 奈緒子, 森田 裕子, 藤里 俊哉, 岸田 晶夫
    54Annual 巻 (2016) 27AM-Abstract 号 p. S162
    公開日: 2016/11/19
    ジャーナル フリー

    In this study, a decellularized porcine aorta having long length was converted to a small-diameter vascular graft and then covered with electrospun segmented polyurethane (SPU) fibers in order to reinforce its mechanical property. The intima-media of aorta was decellularized using high hydrostatic pressure method. The decellularized intima-media was formed cylindrically and covered with SPU by using our original electrospinning apparatus. For SEM observation, two layers of inner decellularized intima-media and outer SPU fibers were observed. The thickness of SPU fibers was increased with increasing of electrospinning time. The extensibility of the hybrid blood vessel was inhibited by covering of SPU fibers. Also, the stiffness of them was similar to that of native blood vessels by controlling the thickness of SPU fibers. From these results, the compliance matching the hybrid blood vessel could be applicable as a novel small-diameter vascular graft.

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  • 森 光一, 寺口 博也, 小倉 亮介, 中村 真人, 野口 京
    54Annual 巻 (2016) 27AM-Abstract 号 p. S163
    公開日: 2016/11/19
    ジャーナル フリー

    Magnetic resonance imaging (MRI) is one of the most promising non-invasive methods to evaluate the organ functions. Especially, arterial spin labeling (ASL) method provides the information of the blood perfusion status without using contrast media. And diffusion weighted image (DWI) method, which can detect the diffusion of the water of tissue and organ, has a potential to reflect the perfusion status, too. However, we cannot distinguish perfusion from diffusion exactly by boxel size in vivo. Then, we tried MRI analysis by ASL and DWI, using the extracorporeal perfusion model of the pig kidney isolated after slaughtering. An electrolyte solution was perfused at the pressure of 30mmHg from the artery by drip infusion, and the perfusion study was carried out by ASL and DWI. As a result, although neither typical perfusion nor infarction clues were detected by ASL, a slight clue of perfusion could be detected by DWI.

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