This study investigated the feasibility of serial digestion of aquatic plant biomass under different mesophilic HRT conditions in semi-continuous stirred tank reactor (CSTR), and in solid-state batch post digestion experiments.
Elodea nuttallii was used as substrate. The experiments were conducted in 3.0 L CSTR with organic loading rate (OLR) of 4.08 and 2.04 g-VS L
-1d
-1 for HRT 15 and 30 days, respectively. Centrifuged digestates at the end of the experiments were used as substrates in batch post solid-state experiments. The biomethane potentials were tested in Automated Methane Potential Test System (AMPTS II). The pH on HRT 15 days decreased to 5.0 on day 24, but recovered to optimum values of 6.81–7.44 from the 64 of the experiment. In HRT 30 days’ condition, pH values were maintained at suitable values (6.86-7.73) throughout. In a steady state, the CH
4 yield were 161 and 234.3 mL g-VS
-1 for HRT 15 and 30 days, respectively. In post-AD, 55.0 and 54.4 mL g-VS
-1 were recovered on HRT 15 and 30 days, respectively. In total, CH
4 recovered from wet-AD plus post-AD were 216.0 and 288.7 mL g-VS
-1 for HRT 15 and 30 days, respectively. Solid-state post digestion experiment improved methane recovery from wet-AD.
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