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Volume 43, Issue 1
Displaying 1-8 of 8 articles from this issue
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Kazuki KANAZAWA1990 Volume 43 Issue 1 Pages 1-15
Published: December 10, 1990
Released on J-STAGE: February 22, 2010
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Comparison of Fluorescence Intensity between Diabetic Subjects and NormalHiroaki HORIKAWA, Noriki NAGAO, Ryuko MATSUDA, Eiko ITO, Sumie MANNO, ...1990 Volume 43 Issue 1 Pages 17-22
Published: December 10, 1990
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSHuman plasma albumin isolated from normal subjects exhibited a characteristic fluorescence with an emission maximum at 450nm, when excited at 346nm. Incubation of these plasma samples over a period of 20 days in the presence of glucose at physiological concentrations in vitro resulted in an increase of fluorescence intensity accompanied by a gradual shift of the emission maximum to 430nm. Each serum albumin sample isolated from 18 diabetic subjects also exhibited similar fluorescence spectra to those observed in normal subjects. In addition, it was revealed that the mean value of the fluorescence intensity per milligram of albumin in the diabetics was significantly greater than that in the normals.View full abstractDownload PDF (2357K) -
Katsuhide OKADA, Masaru YONEYAMA, Takahiko MANDAI, Hajime AGA, Shuzo S ...1990 Volume 43 Issue 1 Pages 23-29
Published: December 10, 1990
Released on J-STAGE: March 25, 2010
JOURNAL FREE ACCESSThe hydrolysis in the digestive tract of pullulan, a microbial polysaccharide, was examined by an in vitro digestion method. Pullulan was partially hydrolyzed by salivary and pancreatic amylases without glucose formation. No hydrolysis was observed using artificial gastric juice. Small-intestinal enzymes hydrolyzed pullulan to lower molecules, producing a small amount of glucose (2.7%). Microbial fermentation of pullulan in the large intestine was examined using an in vitro fecal culture system. Pullulan was fermented to short-chain fatty acids (mainly acetic, propionic and n-butyric acids) as the hindgut fermentation products. Under anaerobic incubation, the carbon recovery of short-chain fatty acids was 47.8%. The behavior of pullulan in the digestive tract was thus clarified using this digestion method and fecal culture system.View full abstractDownload PDF (1215K) -
Mieko KIMURA, Yoshinori ITOKAWA1990 Volume 43 Issue 1 Pages 31-42
Published: December 10, 1990
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSTo assess loss of the minerals; Na, K, P, Ca, Mg, Fe, Zn, Mn, Cu during cooking, their contents in food were determined before and after cooking. The results obtained were as follows. 1) The mineral contents in cooked food were on average about 75% of those in raw or uncooked food. 2) Among various cooking methods, loss of minerals was largest for squeezing after boiling and for soaking in water after thin slicing, followed by parching, frying and stewing. 3) Cooking losses were almost the same for both large-scale and home cooking. 4) Measures for preventing cooking loss are (1) eating with soup after boiling, (2) addition of a small amount of salt (about 1% NaCl) when boiling, (3) avoidance of excessive boiling, and (4) selection of a cooking method causing less mineral loss (stewing, frying or parching).View full abstractDownload PDF (1904K) -
Chemical Changes in the Fermentation Medium and Mackerel Meat during Fermentation and the Production of Volatile ComponentsYasuhiro KARIYA, Ritsuko KIUCHI, Naomi MIKAMI, Hitomi DOISHITA, Kenkic ...1990 Volume 43 Issue 1 Pages 43-48
Published: December 10, 1990
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSSaba-narezushi (mackerel and pickles), a fermented fish product from the Wakasa bay area of Fukui Prefecture, wasprepared in the laboratory using desalted heshiko (salted mackerel cured with rice bran), koji (ricemalt), and boiled rice according to the traditional process, and analyzed for its contents of reducing sugar, organic acids, amino acids, alcohols and esters. After fermentation, reducing sugar accounted for about 4.2% by weight of the fermentation medium, and organic acids for about 1%, and amino acids about 0.95%. Lactic acid and citric acid were found as the major acids and acetic acid as a minor volatile acid. Major volatile compounds found by gas chromatography in the fermentation medium were ethanol and ethyl acetate.View full abstractDownload PDF (1721K) -
Munehiro YOSHIDA1990 Volume 43 Issue 1 Pages 49-53
Published: December 10, 1990
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSThe effect of dietary intake of selenium-rich food (bread, meat, eggs and fish) on plasma, erythrocytes and urinary selenium concentration was studied. plasma, erythrocytes and urinary selenium levels in healthy male (n=25) and female (n=13) subjects were determined fluorometrically and the weekly intake of seleniumrich foods was assessed using a simplified questionnaire. Geometrical mean values of selenium levels were as follows: plasma (ng/ml), 122 (male) and 118 (female); erythrocytes (ng/ml), 233 (male) and 175 (female); urine (μg/g creatinine), 25 (male) and 30 (female). Erythrocyte selenium concentrations were significantly correlated with weekly fish intake (r=0.411, p<0.05 (female+male) or r=0.453, p<0.05 (male)). Neither plasma nor urinary selenium concentrations were correlated with weekly intake of selenium-rich foad. Intake of seleniumrich food at breakfast on the day of blood or urine sampling and at supper on the preceding day were also assessed by the questionnaire. Subjects who consumed two or more selenium-rich foods at these two meals showed higher urinary selenium excretion than other subjects. These results indicate that the dietary habit of fish consumption is the main factor influencing the erythrocyte selenium concentration in Japanese, and that urinary selenium excretion is altered by intake of selenium-rich food before urine sampling.View full abstractDownload PDF (1123K) -
Hirotomo OCHI, Shigeru SHIGEOKA, Yoshitomi IIZUKA, Fumio WATANABE, Yos ...1990 Volume 43 Issue 1 Pages 54-57
Published: December 10, 1990
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSTo elucidate the requirement of inorganic ions in Euglena gracilis z, the organism was grown in medium depleted of each of the following metal ions: Mg2+, Ca2+, Mn2+, Cu2+, Co2+, Ni2+, Fe3+. Euglena cell growth was depressed by depletion of Zn2+, Mg2+, Mn2+, or Fe3+, but not by that of Ca2+, Cu2+, Co2+, or Ni2+, indicating that Euglena has an absolute requirement for at least Zn2+, Mg2+, Mn2+, and Fe3+ for growth. Euglena was grown in medium supplemented with a large amaunt of each metal ion (10 or 100 times that in original Koren-Hutner medium) to study ion uptake and accumulation. Euglena cell growth was significantly depressed by addition of 100-fold excess Ni2+, Co2+, Mg2+, or Ca2+. However, Cu2+, Mn2+, Zn2+, Ca2+, and Fe3+ were taken up by the cells and accumulated at levels 130, 30, 16, 11, and 3 times higher than those in cells grown in the standard medium, respectively.View full abstractDownload PDF (745K) -
[in Japanese]1990 Volume 43 Issue 1 Pages 58-61
Published: December 10, 1990
Released on J-STAGE: February 22, 2010
JOURNAL FREE ACCESSDownload PDF (993K)
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