日本栄養・食糧学会誌
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53 巻 , 2 号
選択された号の論文の5件中1~5を表示しています
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  • 小澤 慶子, 小林 嘉一郎, 加藤 保子
    53 巻 (2000) 2 号 p. 59-64
    公開日: 2009/12/10
    ジャーナル フリー
    全卵からアレルゲン活性の高いOMを除去する方法を検討するとともに, そのアレルゲン活性を患者血清を用いたELISA法で評価した。
    1) 卵黄と分離した卵白を沸騰させた0.8%-NaCl溶液中に少量ずつ添加して, 15分間加熱して凝固卵白を得た。これを粉砕, 撹拌洗浄してEW (-OM) を調製した。卵黄は, 生卵黄を濾紙で覆い, 卵黄膜に付着する卵白の混入を防ぎながら卵黄内容物のみを抽出した。
    2) 凝固卵白と卵黄内容物の混合物 (Egg (-OM))を緩和な条件 (98℃, 5分間) で加熱した。加熱Egg (-OM) から塩溶性タンパク質および全タンパク質を抽出し, SDS-PAGEおよびマウス抗OM血清を用いた Immunoblotting および競争阻害ELISA法で, OMが残存しないことを確認した。
    3) 加熱Egg (-OM) のアレルゲン活性を鶏卵アレルギー患者血清を用いて競争阻害ELISA法で調べた。EW (-OM) のアレルゲンは, ほぼ完全に消失したが, 加熱Egg (-OM) には, OMの阻害活性が残存した。
    4) 加熱Egg (-OM) の卵黄タンパク質に対するアレルゲン活性は失われたものの, カゼインと抗カゼイン特異IgEとの反応をわずかに阻害した。したがって, IgEと反応する要素が卵黄中に存在することがその要因の一つであると考察した。
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  • 園山 慶
    53 巻 (2000) 2 号 p. 65-70
    公開日: 2009/12/10
    ジャーナル フリー
    Apolipoproteins play a central role in transport and metabolism of lipids in animals. We investigated the regulatory mechanisms responsible for expression of apolipoproteins in the small intestine. Animal experiments using surgical procedures suggested that the biliary component is a luminal factor regulating apolipoprotein A-IV expression in the intestine. In addition, peptide YY stimulated the apolipoprotein A-IV expression in Caco-2 intestinal cells in a dose- and time-dependent manner, suggesting that peptide YY is a humoral factor regulating the expression of apolipoprotein A-IV. Furthermore, intravenous infusion of hexamethonium and atropine, but not propranolol, diminished apolipoprotein A-IV mRNA levels in the rat ileum, suggesting the presence of basal cholinergic stimulatory expression of the apolipoprotein A-IV gene in the ileum. In conclusion, we propose that expression of apolipoprotein A-IV in the intestine is regulated by luminal, humoral, and neural factors. We also discuss the relationship between the hypocholesterolemic action of dietary beet fiber and hepatic expression of the apolipoprotein A-I gene in rats.
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  • 加藤 範久, 栢下 淳, 佐々木 真宏
    53 巻 (2000) 2 号 p. 71-75
    公開日: 2009/12/10
    ジャーナル フリー
    Although buckwheat protein has high biological value, its digestibility is relatively low. We have found that this low digestibility is closely associated with its hypocholesterolemic activity in cholesterol-fed rats. This prompted us to investigate the possibility that buckwheat protein has dietary fiber-like effects, including anti-constipation, anti-obesity, and anti-tumor effects. The results we obtained supported this possibility. The silk protein, sericin, is also resistant to several proteases, and has a number of physiological functions such as strong water-holding capacity and antioxidant activity. These properties suggest that it would exert protective effects against constipation and colon carcinogenesis. In view of these facts, we postulated that low digestibility of dietary protein might improve the functions of the intestine and be beneficial for human health. By drawing an analogy with resistant starch, we propose that buckwheat protein and sericin might be “resistant proteins”, and discuss their physiological significance.
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  • 松井 利郎, 川崎 晃一
    53 巻 (2000) 2 号 p. 77-85
    公開日: 2009/12/10
    ジャーナル フリー
    The procedures for preparing a food material with antihypertensive efficacy from sardine muscle have been reviewed. Hydrolysis of sardine muscle by pepsin yielded angiotensin I-converting enzyme (ACE)-inhibitory peptides. Further studies aimed at preparing a hydrolyzate with potent ACE-inhibitory activity as well as having good flavor were carried out using Bacillus licheniformis alkaline protease. After 17h of hydrolysis of 0.3wt% sardine protein, the 10wt% ethanol fraction obtained with ODS resin showed some excellent properties, including strong ACE-inhibitory activity (IC50=0.015mg protein/mL), high digestive resistance, no bitterness, and no antigenicity. In addition, this fraction was confirmed to have a significant in vivo depressor effect in mildly hypertensive volunteers (n=29), who showed a 9.3mmHg reduction in systolic blood pressure and 5.2mmHg reduction in diastolic blood pressure after 4 weeks of oral administration of this peptide drink (4g of the ODS fraction/200mL/day).
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  • 斎藤 衛郎, 高橋 敦彦, 武林 亨
    53 巻 (2000) 2 号 p. 87-94
    公開日: 2009/12/10
    ジャーナル フリー
    The intake of dietary fiber necessary for amelioration of hypercholesterolemia and prevention of ischemic heart disease and diabetes mellitus was tentatively assessed by searching articles from databases on the Internet such as Medline, Chemical Abstracts, etc. The values obtained were much higher than those in the Recommended Dietary Allowances for the Japanese, Sixth Revision, in which 20-25g/day (10g/1, 000kcal) is set as the adequate dietary intake level for adults. In view of the average intake of dietary fiber among Japanese people, which is assumed to be about 16g/day, efforts should be made to attain at least the recommended level. In addition, as the amelioration and prevention of the above disorders are accomplished by water-soluble dietary fiber, the intake of this type of fiber, as well as total dietary fiber, should be increased.
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