A basal diet was prepared using mainly vegetable materials, vitamins, minerals and anti-b iotics. Each 15 young chicks were fed on the experimental diet in a wire cage for 30 days The growth rate and feed efficiency were increased about 6% by the addition of 0.3% aspartic acid. But the viscera distribution and the activities of xanthine dehydrogenase and cholin oxidase of liver were scarcely influenced. The basal diet was supplemented with or without tryptophan, lysine, both of them, orad istillers feed to prepare 6 kinds of diet, and 0.3% of aspartic acid or glycine was added. There were no differences in the growth rate, feed efficiency and viscera distribution on average of feeding experiments, but the weights of testes of the aspartic acid groups were a little larger.
Postoperative metabolism is characterized with marked nitrogen output and catabolism. Therefore, determination of the essential amino acid requirement in the postoperative period required special considerations on postoperative metabolism, even in the animal experiment. In this study these criteria were examined in various conditions. The following conditions were found to be acceptable to determine the requirement of the essential amino acids in the postoperative period. 1) Male rat with an age of over 3 months and weight of 100-150g should be used. 2) During observation the most suitable amount of diet was 10g of 10% casein synthetic diet per day. 3) Among various surgical interventions, third degree of burn covering 20% of body surface was suitable in extent of surgical intervention, uniformity and low surgical mortality. 4) Essential amino acid requirement was best determined by weight changes. In young growing male rats (100-150g) subjected to third degree of burncoveting 20% of body surface, the supplementation effects of methionine, lysine plus methionine, and lysinemethionine plus tryptophan on weight changes in the postoperative period were studied under the previously mentioned conditions. The requirement of methionine did not increase and that of lysine increased in the postoperativeperiod.In rats fed diet containing lysine 2 times as much as normal requirement, weight loss at 4th postoperative week was less by 10g than in those fed diet containing normal requirement of lysine. The supplement of methionine in the postoperative period was not much effective. In the postoperative period, the amount of protein in the diet was markedly related to postoperative changes in weight.
In practical application of the idea of ideal postoperative essential amino acid ratio, that ofth e adult is more important than that of young (growing period). In this postoperative requirement of essential amino acid was studied in adult rats subjected to third degree of burn covering 20% of body surface.(1) Recovery from the burn was more rapid in growing rats (2 weeks) than in adult (3 weeks). The weight loss in the postoperative period continued for 1 week in growing rats and for 3 weeks in adult. The disturbance in metabolism due to surgical intervention was more marked in adult rats than in growing rats.(2) In adult rats, postoperative weight loss at 3 weeks was 20g in those fed 12g of 10% casein diet with 0.10g of glycine per day, and 9g in those fed 12g of 10% cesein diet with 0.6g of lysine.(3) The optimal postoperative requirement of lysine in adult rats was 2 times as much as that in normal metabolic state.
The storage experiment was carried out under low temperature, comparing with natural conditions, from Feb. 1959 to Sept. 1960 in order to investigate the changes of chemical qualities in husked and white rices. Two controlled conditions as (1) below 15°C and about 70% in relative humidity and (2) below 10°C and about 80% in relative humidity were employed. As the result of experiments, the following advantages of low temperature storage were recognized. (1) Decrease of enzymatic activities and thiamine content were less during the storage, and stored husked rice kept quality as almost same as in the starting period of storage. (2) The observed characteristics in the amylograms of white rice and reducing sugar, showed that changes of carbohydrate were slight. (3) Cooking qualities of white rice were almost similar to that of rice when the storage was started. (4) The change in the amount of water soluble nitrogen in husked rice was very little.
To evaluate the effect of the mushroom on cholesterol metabolism, rats were fed ad libitum for 10 weeks with a diet containing either 5% dried mushroom or 0.5% ergosterolwith or without exogenous cholesterol. The results indicated that the dried mushroom couldremarkably reduce the plasma cholesterol but did not reduce the liver cholesterol. On theother hand, the rats fed with a diet containing ergosterol showed high cholesterol contentsin both plasma and liver. These results suggest that the effect of mushroom on the plasmacholesterol is not due to the ergosterol but due to some other substances contained in themushroom.
Equal amount of serum is injected to the blood-lettened rabbits and the following results were obtained. The icrease of serum fats by blood-letting is mainly caused by that of saturated fatty acids and oleic acid in triglycerides. This was prevented by serum injection.
Rats were given same kind of pantothenic acid defficient diets. Depending on season and characteristic of each rat there was a great difference in the number of days in whichsymptoms appeared and degrees of symptoms. Accordingly it is very difficult to determinethe minimum curative doses of pantothenic acid derivative. 1) When treatment was begun as soon as symptoms appeared, the rats quickly recoveredafter given 30μg D-calcium pantothenate or D-pantothenol daily. In cases of DL-derivativesabout double the doses were necessary. 2) When rats with a typical high degree of pantothenic acid defficient symptoms were given30μg D-pantothenic acid derivatives daily, although the symptoms showed slight recovery, they finally died. 3) In the treatment of experimental pantothenic acid deficiency a single large dose is moreeffective than several small doses. 4) As central nervous disturbances were often observed in pantothenic acid defficient rats, we believe they are one of the pantothenic acid defficient symptoms. The peripheral nervousdisturbances always appearing are mainly hyper-algesia, however the central nervousdisturbances are mainly extrapyramidal lesion.