The toxicities of histamine and phenylethylamine in the rotten matsutake mushrooms were already reported in the last paper Part 7. Now kittens were used to make it sure how histamine and phenylethylamine would cause animals to vomit. 1) Kittens vomited immediately after histamine was given through their mouths, the least necessary quantity being 15mg/kg. 2) Phenylethylamine had nothing to do with the vomit. But it caused a delatation of kitten's eyes, the degree of which varied on the quantity of phenylethylamine. These symptons were acute and did not last long.
Smith claims in 1942 that the creatinuria of women is exaggerated during premenstrual period, but he did not take the effect of diets into consideration. The authors reexamined the relation between the excretion of creatine and the periods of menstrual cycle with three women, who continued to take the same diets, throughout the experimental period. The daily variation of the excretion of creatine has been so considerable that a definite relation between the creatinuria and the period during the menstrual cycle could not be found. Contrary to expectation, the urinary estrogen also had not a constant relation with the menstrual cycle.
For the coloring of water-base foods especially of juices and beverages with β-carotene, the oily emulsion of β-carotene is most preferable. For this purpose, solubilities of β-carotene in various solvents were measured, obtaining the following results. 1. β-Carotene once dissolved in orange oil by heating remained about 3% in solution after standing at room temperature for as long as one month. 2. When the solution of β-carotene in orange oil was heated, isomerization and degradation occurred. In general, the higher the heating temperature and the longer the heating period, the more the cis compound was formed. When 1% suspension of all-trans β-carotene in orange oil was heated at 100° for 30 minutes, 60-65% remained as all-trans form, although 35-38% was isomerized to Neo-B.
The coloring of soft drinks with emulsified β-carotene was studied with the following results. 1. The stability of emulsified β-carotene was practically excellent. 2. The presence of oxygen was the most important factor to affect the stability of β-carotene in the emulsion, and the light accelerated the destruction of β-carotene in the presence of oxygen. 3. Copper ion greatly affects the stability. 4. The orange soft drinks colored with the emulsion did not show any adverse effect. 5. Little color change was observed when orange soft drinks fortified with ascorbic acid was colored by the emulsion and stored at least for a year.