Journal of Japanese Society of Oral Implantology
Online ISSN : 2187-9117
Print ISSN : 0914-6695
ISSN-L : 0914-6695
Volume 8, Issue 1
Displaying 1-12 of 12 articles from this issue
  • Yasushi Inoue
    1995 Volume 8 Issue 1 Pages 1-10
    Published: March 31, 1995
    Released on J-STAGE: February 15, 2018
    JOURNAL FREE ACCESS
    Repair of bone defect is one of the most important factors that must be settled in dentistry. Recently, a number of laboratories succeeded in isolating bone morphogenetic protein(BMP)and producing their recombinant proteins. Bone morphogenetic protein is known to be protein which induces new bone at heterotopic sites. Osteoinductive activity for bovine BMP fraction has been demonstrated in rats, so the isolates are not species-specific. This suggests that BMP are cytokine to solve the problem in bone repair. In the present study, we purified partially BMP isolated from bovine bones by heparin affinity chromatography. The osteogenic activity of isolated BMP fractions was examined in vivo on a composite of collagen and PEC.
    Download PDF (3996K)
  • Koichiro Ihara, Masaaki Goto, Eiro Kubota, Takeshi Katsuki
    1995 Volume 8 Issue 1 Pages 11-17
    Published: March 31, 1995
    Released on J-STAGE: February 15, 2018
    JOURNAL FREE ACCESS
    Due to the rapid development of antibiotics, osteomyelitis has become an unusual disease. But once this is encountered, it takes a lot of time to treat it, and often results in bone defects and functional disturbances.
    This is a report of a case in which occlusal rehabilitation was performed by using bone graft and osseointegrated implants after treatment of osteomyelitis. A 64-year-old male with osteomyelitis of the mandible was treated by intraarterial or intravenous infusion of antibiotics. Sequestrectomy was performed after the active infection disappered. Mandibular defect was reconstructed by titanium-mesh-plate and autogeneous iliac bone grafting. After 3 years of follow-up, occlusion was reconstructed by using Brånemark Implant prosthesis. Bone sintigram was revealed to be a very effective marker for evaluating the treatment effects and for diciding the timing of implant installation.
    Occlusal rehabilitation using implant-supported prosthesis is an unusual treatment for the patients who suffered from osteomyelitis. This patient, however, was successfully treated by sequestrectomy and bone graft without reccurence of the disease. The occlusal rehabilitation of the mandible by implant may contribute to the patient's quality of life(QOL).
    Download PDF (2744K)
  • Susumu Yamane, Makoto Minamimoto, Nobuyuki Imoto, Tomohiko Matsuda, Ko ...
    1995 Volume 8 Issue 1 Pages 18-24
    Published: March 31, 1995
    Released on J-STAGE: February 15, 2018
    JOURNAL FREE ACCESS
    For more than 4 years, 4 splinted bar-retained overdentures with 4 implant abutments were observed, and Periotest ® values(PTVs)of a total of 16 implant abutments were measured.
    The mean PTV of bar-removed implants was -3.81±0.52(S.D., n=16), independent of the place and period of the implantation.
    That of bar-retained implants was -4.90±0.77(S.D., n=16). PTVs of implants of the ends were the same as those of bar-removed implants, but in the 2 central implants, the significant differences between PTVs of bar-retained and removed implants were recognized at 2% risk.
    Download PDF (2271K)
  • ―Considerations from Clinical Results and Model Examination―
    Tetsuo Ichikawa, Masanobu Horiuchi, Yasuyuki Horisaka, Hideo Kanitani, ...
    1995 Volume 8 Issue 1 Pages 25-31
    Published: March 31, 1995
    Released on J-STAGE: February 15, 2018
    JOURNAL FREE ACCESS
    It is important for implant success to evaluate bone quality in the region of implant placement. The relation between preoperative bone examinations and the apatite implant prognosis were examined. Two preoperative bone examinations were conducted:1)subjective estimation at pilot-hole preparation, which one operator classified bone quality as two types as “hard” and “soft,” and 2)bone density in the radiographic image using standardized radiography and digital image processing. Two estimations of implant prognosis were done:1)clinical estimation, which one operator classified clinical prognosis into three types, “good,” “problematic,” and “failure,”and 2)marginal bone loss for first one year. Further, we conducted the drop test using implant-bone models simulating both hard and soft bone. The relation between bone quality and implant prognosis found in the two above experiments were discussed.
    As a clinical result, no significant relation between preoperative bone examinations and implant prognosis was found. Hard quality bone had a little tendency to be caused to problematic implants.
    “Resin model” was assumed to be hard quality bone and “Hybrid model” was assumed to be soft quality bone. A small accelerometer was attached to the abutment and a strain gauge was placed on the surface of the model. A metal rod was dropped on the superstructure. Both abutment acceleration and surface strain in the resin model were lower than those in the hybrid model. Both abutment acceleration and surface strain in the hybrid model decreased rapidly with time as compared to those in resin model. Influence of loading sites on the strain in the resin model was larger than that in the hybrid model.
    It seemed that hard bone may be a high risk factor for implant failure.
    Download PDF (2219K)
  • ―Study on Using Double Immunohistochemical Technique―
    Morio Ochi, Kunihiko Sakaguchi, Yukito Hirose, Hiroyuki Matsumoto, Nor ...
    1995 Volume 8 Issue 1 Pages 32-39
    Published: March 31, 1995
    Released on J-STAGE: February 15, 2018
    JOURNAL FREE ACCESS
    Prompt osseointegration after placement of endosseous implant can hasten the treatment period before seating the final superstructure and contribute to the prompt restoration of the patient's occlusion. We developed the idea of using stimulation with pulsing electromagnetic fields(PEMFs)as a method for increasing the rate of osseointegration, and have previously performed related experiments in vitro and vivo.
    At the 23rd Congress of the Japanese Society of Oral Implantology, we reported the results of the immunohistochemical investigation using BrdU and PCNA as markers to study the proliferation dynamics of MC 3 T 3-E1 cells stimulated by PEMFs. This stimulation was found to increase cells in S-phase and late G1 to S-phase.In the present experiment,the double immunohistochemical staining technique with BrdU and PCNA, recently reported by Connolly and Bogdanffy, was used to double-stain MC 3 T 3-E1 cells stimulated by PEMFs, and a comparison for the usefulness of this technique to single staining with BrdU or PCNA was performed.
    Results of the study indicated the following:
    1. After single-staining with BrdU and PCNA, the BrdU labeling index and PCNA labeling index distinctly increased in cells in the experimental group (p<0.001). Indices for cells in S-phase and late G1 to S-phase were both 1.5 times higher than those in the control group.
    2. After double-staining with BrdU and PCNA, there was a distinct increase in the labeling index of BrdU-PCNA-positive cells in the experimental group(p<0.001), which was 1.3 times higher than that in the control group.
    3. In addition to cells that were double-stained with BrdU and PCNA by the double-staining technique, some cells were stained with PCNA alone.
    4. After single-staining with BrdU or PCNA, Some differences in staining intensity were noted, suggesting that the criteria defining positive cells may be influenced by the subjective judgment of the investigator. This indicates that the human related factors greatly effect the reproducibility and consistency of the measured data. However, since the double-immunohistochemical staining technique simultaneously employs two types of stains, positive cells are stained darker than they are by single-staining, making the calculation of cells in S-phase more objective.
    In the future, we intend to further investigate the clinical application of PEMFs stimulation in the field of dentistry, including oral implantology.
    Download PDF (2791K)
  • Ikuhisa Inada, Yasuhiro Matsuzuka, Katsuhiro Horiuchi, Masahito Sugimu ...
    1995 Volume 8 Issue 1 Pages 40-47
    Published: March 31, 1995
    Released on J-STAGE: February 15, 2018
    JOURNAL FREE ACCESS
    Recently, dental implants have been widely used for dental rehabilitation. In some cases, however, insufficient planning and complications result in poor prognoses. This report presents our experience of removing unfavorable dental implants installed in other clinics. Fourteen patients were treated at our department between October 1982 and June 1994. The patients were 7 males and 7 females, whose ages ranged from 41 to 72 years, with an average age of 58.3 years. Eight patients had both maxillary and mandibular implants, four only maxillary ones and two only mandibular ones. In patients with maxillary implants, eleven had intra-osseous implants, one subperiosteal implant,and one of both types. All mandibular implants were intraosseous. All intraosseous implants had been installed by the one-stage procedure. The diagnosis was alveolitis in seven patients, maxillary sinusitis in nine, oroantral fistula in three, and osteomyelitis of the mandible in one. Maxillary sinus curettage was performed in five patients, antrostomy in three, sinus irrigation in one, fistulectomy using a palatal flap in three, and apically repositioned flap operation in one. It is concluded that careful planning and ceaseless follow-up are necessary to avoid complications, especially in atrophic jaw bones.
    Download PDF (2886K)
  • Kazushi Murata, Isumi Toda
    1995 Volume 8 Issue 1 Pages 48-59
    Published: March 31, 1995
    Released on J-STAGE: February 15, 2018
    JOURNAL FREE ACCESS
    Dental implant operation is usually performed following a complete healing of the tooth extraction socket and the superstructure is fixed after a definite resting period. However, if implantation would be performed immediately after tooth extraction, healing of the extraction socket proceeds simultaneous with osteointegration surrounding the implant. Such an attempt will shorten implant operation as a whole.
    In the present study, mandibular molars on one side in Japanese monkeys were extracted and those on the other side also extracted. Titanium cylinder type implants were installed 3 months after healing of the sockets in the former as usual. The same implants were installed immediately after extraction in the latter. In both procedures, these animals were euthanasized at 2, 4, 8 and 12 weeks after implantation, respectively. Microvasculature of these materials were examined utilizing the plastic injection method by scanning electron microscopy. The contact interface between the implant surface and integrating bone was examined by transmission electron microscopy.
    In usual implantation, new bone formation began 2 weeks after operation, the whole implant was surrounded by new bone at 8 weeks and the bone matured at 12 weeks. In immediate implantation, newly-formed capillaries were formed around the implant at 2 weeks, new bone formation at 4 weeks and the whole implant was surrounded by new bone at 12 weeks. Collagen fibers expanding from the implant surface were observed at 12 weeks.
    Implant anchorage, even in cases of immediate implantation following tooth extraction, was obtained by sufficient osteointegration at 12 weeks without marked differences from the usual implantation. Accordingly, it can be said that the immediate implantation after tooth extraction in this experimental study is sufficient to make the implantation treatment shorter as a whole compared with usual implantation.
    Download PDF (4923K)
  • Nobuyuki Manaka, Mie Kaneko, Toshiharu Fujii, Hiroaki Kataumi, Hiroyuk ...
    1995 Volume 8 Issue 1 Pages 60-68
    Published: March 31, 1995
    Released on J-STAGE: February 15, 2018
    JOURNAL FREE ACCESS
    Based on the endosteal implant postoperative evaluation system in our department, the cases to which titanium bladevent implants were applied were studied for more than 5 years postoperatively. The evaluation was made on 30 of 80 cases who visited our clinic for examination. The evaluation system included the study on:pocket depth around the implant neck, gingival condition, implant mobility, radiographic examination for resorption of alveolar bone, and patient's satisfaction. And the cases were classified as Class 1(good), Class 2(fair), Class 3(removal necessary), and Class 4 (removed).
    The results were:18 cases in Class 1(60%), 9 cases in Class 2(30%),1 case in Class 3(3.3%), and 2 cases in Class 4(6.7%). The cases of Class 1 and 2 showing satisfactory course accounted for 90% and removal cases occurred within one year after operation. These results suggest that it is important to observe the implant within one year after operation.
    Download PDF (3038K)
  • Yasunori Hotta, Hidehiro Suzuki
    1995 Volume 8 Issue 1 Pages 69-82
    Published: March 31, 1995
    Released on J-STAGE: February 15, 2018
    JOURNAL FREE ACCESS
    Subperiosteal implants and sinus elevation are indicated for the treatment of patients with severe vertical bone resorption in the maxillary molar region because the lack of sufficient vertical bone between the floor of the maxillary sinus and the alveolar crest precludes the use of endosteal implants. This report will describe the case histories of four patients to illustrate different options for implant therapy. Subperiosteal implants and sinus elevation were each found to have their own set of problems. Therefore, patients indicated for these techniques must be carefully selected. It was found that these techniques provided a good short-term outcome clinically and follow-up observations are being performed to evaluate the long-term results of therapy.
    Download PDF (5311K)
  • Hidetaka Nakai, Atsushi Niimi, Toshihiro Sawai, Kazuyo Watanabe, Kenji ...
    1995 Volume 8 Issue 1 Pages 83-87
    Published: March 31, 1995
    Released on J-STAGE: February 15, 2018
    JOURNAL FREE ACCESS
    Evaluation of cases, using osseointegrated implant to retain the denture, before surgery was proposed. The purpose of this study was to establish a systematic evaluation method for a patient who has a maxillary defect and needs osseointegrated implant placement to retain the denture. The evaluation items are as follows:
    ・Quantity and quality of anterior alveolar process (A)
    ・Defect of zygomatic process(Z)
    ・Defect of maxillary tuberosity and pterygoid region(P)
    ・Thickness of bone under floor of maxillary sinus (I)
    ・Total dose of radiation treatment(R)
    The problems of osseointegrated implant placement to retain the denture could be clarified by this evaluation method.
    Download PDF (1415K)
  • Masaki Honda, Atsushi Niimi, Hidetaka Nakai, Kenji Ozeki, Takehiro Fuj ...
    1995 Volume 8 Issue 1 Pages 88-91
    Published: March 31, 1995
    Released on J-STAGE: February 15, 2018
    JOURNAL FREE ACCESS
    It is rather difficult for a patients with Sjögren syndrome to wear the full denture, because the salivary secretion rate decreases. In this report, a case of a patient, who has regained an excellent occlusion after adaptation of the bone anchored bridge to osseointegrated implants, is presented.
    It is suggested that crevicular fluid in implant sulcus is associated with maintenance of the normal condition of gingiva around the implant in spite of the reduction of salivary secretion rate.
    Download PDF (1298K)
  • ―Evaluation with Osteoblast-like-cell―
    Nobuaki Takahashi, Kazu Eida, Yasunobu Hanada, Arata Iguchi, Kenji Fuj ...
    1995 Volume 8 Issue 1 Pages 92-96
    Published: March 31, 1995
    Released on J-STAGE: February 15, 2018
    JOURNAL FREE ACCESS
    A synthetic hydroxyapatite(HAP)of bioactive material is widely used because it has an excellent affinity and bone conduction potential, which remain to be elucidated.
    HAP granules with different compositions were made and transplantation into the tibia in rabbits was performed to study initial bone conductivity and long-term stability. It was suggested that they are affected by the Ca:P ratio of HAP, fusing temperature, Mg contents and surface structure.
    The growth effects for fusing temperature and Mg in osteoblasts(MC 3 T 3-E 1)with caltific activity were studied to determine the HAP affinity for osseous tissues.
    Three kinds of synthetic HAP granules of the same composition, which were heated at 1,100, 1,200 and 1,300℃ of the fusing temperature, were employed. In SEM pictures of each granule before dipping and those of cultured MC 3 T 3-E 1, there were no changes on the surface before and after dipping at 1,100℃, but needle-shaped extractives were observed in MC 3 T 3-E1 at 1,200℃ and 1,300℃.
    Each granule was dipped at the rate of 1 mg/ml of α-MEM and kept in a CO2 incubator for 3 days. Ca, P and Mg contents in the culture solution were measured. Ca and P contents decreased and Mg content increased compared with the control group. MC 3 T 3-E 1 cells derived from the neonatal mouse calvaria on a flat bottom microculture plate with 96 wells(1×104/well)were distributed. α-MEM was adjusted to be 5, 25, 50 and 75% of solution, each of which were cultured again in the medium after adding to 10% fetus cow serum under the conditions of 37℃ and 5% CO2 contents.3H-TdR of 37 kBq was added after 48 hr and the nonirradiated activity of the 3H-TdR uptake of MC 3 T 3-E 1 after 72 hr was measured as synthetic DNA. The 3H-TdR uptake in the group to which conditioned medium was added at 1,100, 1,200 and 1,300℃ further increased as compared with the control with only α-MEM.
    MC 3 T 3-E 1 was cultured in the solution after adding to 100~500 mg/l of Mg and the 3H-TdR uptake was measured. In the experiment, an increase of 3H-TdR uptake in proportion to the Mg was shown.
    In MC 3 T 3-E 1, cell proliferation was induced by the dose-dependent medium, with trace elements that affect osteoblasts. Furthermore, the 3H-TdR uptake of MC 3 T 3-E 1 cells was accelerated by Mg in the solution.
    Download PDF (1638K)
feedback
Top