Purpose: Global longitudinal strain (GLS) is predominantly used in clinical practice. However, measured values differed among vendors. Therefore, the use of vendor-independent strain analysis software is recommended. This study aimed to investigate the influence of differences in the frame rate of DICOM data and values from RAW data when measured using vendor-independent strain analysis software.

Methods: This study included 80 patients (mean age: 68±14 years, 54 males) who had clinical echocardiographic examinations with GE Vivid E95 at the Ultrasound Examination Center of Tokushima University Hospital and images suitable for GLS measurement. GLS measurements from RAW and DICOM data with different frame rates (57 fps and 30 fps) were compared using GE’s analysis software.

Results: The mean GLS of RAW data was 14.7%±4.9% and the mean GLS of DICOM data at 57 fps and 30 fps was 13.2%±4.5% and 13.2%±4.4%, respectively. DICOM data (57 fps and 30 fps) exhibited significantly lower GLS than RAW data. No significant difference was found between measurements in DICOM data with different frame rates, and the GLS values of RAW data and two types of DICOM data demonstrated a good correlation (r＝0.99).

Conclusions: The DICOM data demonstrated significantly lower GLS values than RAW data, regardless of the frame rate differences between the RAW and DICOM data. Notably, the GLS value may be lower than that of RAW data when measuring the GLS using DICOM data.