Biological membranes play critical roles in the cell. However, the membrane functions remain elusive due to their complex and dynamic nature. We developed a lithographically patterned model biological membrane composed of polymeric and fluid phospholipid bilayers on the solid substrate for mimicking the membrane structures and functions in pre-defined geometries. By modulating the densities of polymeric bilayers, the fluid bilayer spontaneously segregated into liquid-ordered (L
o) and liquid-disordered (L
d) bilayer phases, forming a patterned model system of lipid rafts. Membrane-bound proteins partitioned in the membrane regions according to their affinities to lipid raft. Quantitative evaluation of the interactions between membrane proteins and lipid raft is important to elucidate the functional roles of lipid raft. We also constructed 3D biomimetic structures combining a patterned bilayer and micro-/nano-structures by attaching the polymeric bilayer and an elastomer sheet. Such micro-/nano-spaces would provide a versatile platform for highly selective and sensitive biosensing.
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