巻号一覧
10 巻 (1960)
- 6 号 p. 359-
- 5 号 p. 285-
- 4 号 p. 231-
- 3 号 p. 143-
- 2 号 p. 69-
- 1 号 p. 1-
前身誌
10 巻, 5 号
選択された号の論文の6件中1～6を表示しています
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植竹 久雄, 萩原 新1960 年 10 巻 5 号 p. 285-289
発行日: 1960年
公開日: 2010/03/16
ジャーナル フリーSalmonella anatum of group E1 was at first lysogenized with phage ε34 under particular conditions (Uetake and Hagiwara, Nature 186, 261, 1960a; Virus, 1960b in press). The resulting ε34-lysogenic cells (=A(ε34)) with antigens 3, 10 were then lysogenized with one of phages, ε15a, ε15b and εy, all of which are mutants of phage ε15 with abnormal conversion properties (Uetake and Uchida, Virology 9, 495, 1959). The resulting double-lysogenic derivatives A (ε34, ε15a), A (ε34, ε15b) and A (ε34, εy) are resistant to phages C341, ε15vir, ε34vir and ε34vir h, although cells A (ε34, ε15b) still adsorb phage ε34vir and cells A(ε34, εy) adsorb phages C341 and ε15vir.
Agglutination and absorption tests showed that somatic antigens of A (ε34, ε15a), A (ε34, ε15b), and A(ε34, εy) were (3), (10), (15), (34), 3, (10), (15). (34), and (3), (10), (15), (34) respectively, different from each other. These indicate that converting properties of ε15a, ε15b, and εy are different from each other, consistent with the previous findings (Uetake and Uchida, 1959) except the finding that phage εy was revealed to retain some gene (s) responsible for the synthesis of antigen 15. Since the synthesis of antigen 34 is always accompanied by the synthesis of antigen 15, they also indicate that the machinery for the synthesis of antigen 15 is a necessary prerequisite for the formation of antigen 34, as pointed out in previous papers (Uetake and Hagiwara, 1960a, 1960b).抄録全体を表示PDF形式でダウンロード (778K) -
渋木 実1960 年 10 巻 5 号 p. 289-304
発行日: 1960年
公開日: 2010/03/16
ジャーナル フリーThirteen strains of distemper virus were isolated in ferrets from the spleens of 31 dogs clinically diagnosed as distemper. Serial passage in developing chicken eggs was attempted with all the strains and 3 were successfully adapted to this host. Propagation in chicken embryos of the egg-adapted virus was studied under various experimental conditions with one of the isolated strains and inoculation into the yolk sac of embryonating eggs incubated for only a few days was found to give the greatest yield of the virus. Inoculation of active virus of the egg-adapted strain produced immunity in ferrets and dogs as revealed by protection test and serum neutralization test. The relationship of the amount of inoculated virus and the extent of acquired immunity was studied.抄録全体を表示PDF形式でダウンロード (2674K) -
荻原 博1960 年 10 巻 5 号 p. 305-315
発行日: 1960年
公開日: 2010/03/16
ジャーナル フリーThe serological response of guinea pigs to mumps virus was studied following inoculation of active virus of the Enders strain and the electrophoretic study was performed with the antiserums obtained, with the intention of obtaining antiserums to S antigen devoid of V antibodies and those to V antigen devoid of S antibodies.
1. Following intracerebral, intranasal, or intraperitoneal inoculation of active virus guinea pigs developed antibodies to V antigen as well as to S antigen.
2. Guinea pigs which developed V and S antibodies following intranasal inoculation of active virus were injected with S antigen prepared from infected chorioallantoic membrane by centrifugation or V antigen prepared by the ether treatment of virus particles to remove the internal S antigen 53 days following the intranasal inoculation, when anti-V and anti-S levels became very low. The booster injections elicited rapid production of a large amount of antibodies to the homologous booster antigen, whereas a little response to the heterologous antigen was noted. By this method high-titered anti-S serums, almost devoid of anti-V, or vice versa, were obtained.
3. The starch zone electrophoresis revealed the presence of V and S antibodies in the gammaglobulin fraction. V and S antibodies showed a slight difference in electrophoretic mobility, which made the separation of V and S antibodies possible.抄録全体を表示PDF形式でダウンロード (1482K) -
守本 富昭1960 年 10 巻 5 号 p. 316-329
発行日: 1960年
公開日: 2010/03/16
ジャーナル フリーThe virus of Russian spring summer encephalitis (RSSE) multiplied without any cytopathogenic effect (CPE) in cell culture of bovine embryonic kidney. Newcastle disease (ND) virus proliferated with CPE in this cell culture, but was not able to proliferate and produce CPE in cell culture previously infected with RSSE virus. With small doses of RSSE virus it took about 4 days for the majority of cells in culture to acquire the ability to interfere with ND virus. Interfering activity of RSSE virus could be detected earlier with a smaller inoculum of ND virus than with a larger one, but the inoculum size of ND virus did not affected the result when ND virus was given at a certain interval following RSSE virus inoculation or later.
Interference between these two viruses were observed in renal cell cultures of the swine, cat, rabbit, and guinea pig, and not in cell cultures of hamster and chicken kidneys or chick embryo tissues. RSSE virus interfered with fowl plague virus and almost or entirely not with viruses of vaccinia, herpes, equine abortion, Venesuelan equine encephalitis, and bovine infectious rhinotracheitis, and HVJ (hemagglutinating virus of Japan).
Japanese encephalitis virus and Negishi virus also interfered with ND virus in bovine embryonic renal cell culture.
Interference of RSSE virus with ND virus was specifically neutralized by immune serum to RSSE virus.
Based on these findings a procedure was established for detecting and measuring RSSE virus and its neutralizing antibody.抄録全体を表示PDF形式でダウンロード (3280K) -
林 幸正, 平井 篤造1960 年 10 巻 5 号 p. 330-337
発行日: 1960年
公開日: 2010/03/16
ジャーナル フリーペトリ皿の内部で水溶液に培養した, タバコモザイクウイルスに感染したタバコ葉組織の不溶性蛋白, 可溶性蛋白, 非蛋白各窒素化合物の変化を, 接種後2, 4, 6日目に測定し, かつこれらの窒素成分が, チオウラシル・マイトマイシンC・ナラマイシン (アクチザオン類似物質) などの抗ウイルス剤でウイルス増殖が部分的に阻害されたはあい, どのように変動するかを調べた. 抗ウイルス剤を作用させないときには, 可溶性蛋白窒素は感染・非感染ともに接種後2日目に減少し, 非感染葉ではその後も次第に減少するが, 感染葉ではやゝ増加する. この増加は非蛋白窒素の減少に符号し, ウイルス感染力の増加の時期とも一致する. すなわち非蛋白窒素が素材となつてそれが可溶性窒素成分に組み入れられ, かくてウイルス感染力の増加となる.
チオウラシル (濃度25ppm, ウイルス感染力を約90%阻害) およびマイトマイシンC (濃度25ppm, 感染後期に約30%阻害) による処理は, いずれも以上の感染による窒素成分の変化を非感染葉のそれに近づけた. すなわち感染末期に非蛋白窒素の減少が認められず, したがつて可溶蛋白の増加がない. とくに処理による不溶蛋白の増加が注意されたが, これがウイルス前駆体の蓄積を意味するかどうかは決定されなかつた. ナラマイシン (濃度1ppm, 主として感染初期に阻害が起こる) の作用性は前2者と異る. すなわち処理によつて非蛋白窒素が全感染期間を通じて増加し, また不溶蛋白が減少する. この物質は植物に対する薬害が強く, 恐らく葉緑 (不溶) 蛋白を分解し, 一方ウイルス増殖の阻害によつて非蛋白窒素から可溶蛋白への経路が阻止されて, かくて前者の蓄積が起こるものと想像された.抄録全体を表示PDF形式でダウンロード (1211K) -
1960 年 10 巻 5 号 p. 341-358
発行日: 1960年
公開日: 2010/03/16
ジャーナル フリーPDF形式でダウンロード (3334K)
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