巻号一覧
13 巻 (1963)
- 5-6 号 p. 145-
- 4 号 p. 107-
- 3 号 p. 81-
- 2 号 p. 37-
- 1 号 p. 1-
前身誌
13 巻, 1 号
選択された号の論文の5件中1～5を表示しています
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萩原 新, 植竹 久雄, 富山 朔二1963 年 13 巻 1 号 p. 1-4
発行日: 1963/02/20
公開日: 2010/03/16
ジャーナル フリーWhen cells of S. anatum lysogenized with phage ε34 (=A(ε34)) are infected with phage ε15 propagated on S. anatum (=A) at multiplicity of infection (m. o. i.) of 9 or less, it takes longer than 6 hours before phage ε15 establishes itself as prophage. Contrary to this, when cells of A (ε34) are infected with ε15 prophagated on strain A (ε34), prophage state is established in about 3 hours after infection even at m. o. i. of 5. And on the other hand, when cells of A (ε34) are infected with ε15 propagated on strain A at high m. o. i., establishment of prophage state is completed in about 3 hours after infection.
These and other results indicate that lysogenization may be affected by host-controlled variation.抄録全体を表示PDF形式でダウンロード (580K) -
清水 文七1963 年 13 巻 1 号 p. 5-10
発行日: 1963/02/20
公開日: 2010/03/16
ジャーナル フリーA variant strain of Japanese encephalitis virus (Hotta strain) isolated in adult mice from a patient was obtained by the high concentration passage and by the method of passage at limmiting dilution in hamster kidney cell cultures. The variant strain showed a marked loss in virulence upon intracerebral inoculation into adult mice. The characteristics of the variant strain were as follows:
1) The variant strain proved to be avirulent for adult mice when inoculated intracerebrally or subcutaneously but retained virulence for suckling mice inoculated intracerebrally.
2) The growth curve revealed that there was no difference in the pattern of virus multiplication in hamster kidney cells between variant and parent strains. However, the rate of multiplication of the variant strain in adult mouse brain was markedly lower than that of parent strain.
3) The variant strain after back passages in adult mice, not only failed to increase virulence but also could not multiply in adult mouse brain. This variant strain showed reversion to virulence for adult mice after three back passages in suckling mice.抄録全体を表示PDF形式でダウンロード (888K) -
植竹 久雄, 富山 朔二, 萩原 新1963 年 13 巻 1 号 p. 11-15
発行日: 1963/02/20
公開日: 2010/03/16
ジャーナル フリーIn order to clarify more definitely and quantitatively the relationship between host-controlled variation and multiplicity activation (Toyama et al., 1962), proportions of plaque formers to phage-infected cells were determined at various m. o. i. (multiplicity of infection) in infection of cells of Salmonella butantan (=I-1) with phage ε15 propagated on cells of Salmonella anatum (=A), and vice versa. Theoretical proportions were also calculated, under the assumption that each cell may become a plaque former when it receives 2. 3, 4, 5 and/or 6 phage particles or more, and compared to the data observed.
The results showed clearly that cells of I-1, which do not allow multiplication of phage ε15 propagated on cells of A when singly infected, may allow phage multiplication when multiply infected. Similar phenomenon was also observed in infection of cells A with phage ε15 propagated on cells of I-1, however, in this case, very high m. o. i. was required for phage multiplication and the effect of multiplicity activation was to a lesser extent than that observed in the former case.
ε15ts is a mutant of ε15, incapable of reproducing at 37°C while capable at 25°C. Phages ε15ts[A] are capable of contributing to multiplicity activation in infection of cells I-1 with phage ε15 ts vir [A] whereas phages ε15ts[I-1] are incapable, indicating that the helper effect of ε15ts[A] is attributed to nongenetic multiplicity activation but not to marker rescue due to genetic recombination.
When inactivated by ultraviolet light irradiation to ca. 10-5 survival, phages ε15[A] are no longer capable of contributing to multiplicity activation in infection of I-1 with ε15ts+vir[A].抄録全体を表示PDF形式でダウンロード (580K) -
前野 幸一郎1963 年 13 巻 1 号 p. 16-23
発行日: 1963/02/20
公開日: 2010/03/16
ジャーナル フリーDuring studies on the growth characteristics of Myxovirus parainfluenzae 1 (HVJ) in various host systems, it was found that a viral carrier state could be obtained in HeLa cell culture infected with this virus. The nature of the carrier culture was then investigated. Results are as follows.
1. The following two different procedures were found to establish carrier state. a) When egg-grown HVJ was used as inoculum at primary infection, carrier state was observed in long-term cultures following concomitant destruction of cells and new cell growth. b) On the other hand, in HeLa cells infected with HeLa cell-grown virus, carrier state was established readily without any detectable cytopathic changes.
2. In spite of frequent reseeding of the cells, these cultures could be maintained as their noninfected counterparts as long as 15 months up to now, with continuous virus production. No differences were found between normal and carrier HeLa cells in their growth rate and in morphology as far as tested with low magnification microscope.
3. Carrier cultures were highly resistant to infection with a large amount of homologous virus, while they had no resistance to infection with several kinds of heterologous virus, i. e., NDV and poliovirus. It was also found, however, that HVJ could be adsorbed to cells of carrier culture at a same rate as to normal HeLa cells. Interferon-like, virus inhibitory substances could not be detected in the fluid medium of the carrier culture.
4. Investigations with hemadsorption tests and fluorescent antibody technics revealed that only a small proportion of cells in the culture (5 to 10 percent) appeared to contain viral antigens.抄録全体を表示PDF形式でダウンロード (1929K) -
高井 欣子1963 年 13 巻 1 号 p. 24-34
発行日: 1963/02/20
公開日: 2010/03/16
ジャーナル フリーThe neutralizing antibody levels was followed for 1 year in 118 infants given the Cox trivalent poliovirus vaccine and for 21/2 years in 5 infants given the monovalent vaccine and 10 children infected indirectly by these 5 infants. A peak was found at 1-3 months and there was a gradual decline thereafter.
The Cox trivalent vaccine was administered in 145 infants divided into 3 groups. The vaccine was given 2 times at intervals of 3 months, 6 months and 1 year. A satisfactory rise in antibody titer was noted in each group suggesting that when the vaccine is to be given 2 times, there is no special need to consider the interval between the administration. It was also found at the time of the second feeding that there was a close relationship between the rise in antibody titer and excretion of virus in the stool.
Children immunized with the live poliovirus vaccine were given booster injections of Salk vaccine but a further rise in antibody was not obtained.
The effect of oral feeding of live poliovirus vaccine after a single injection of Salk vaccine was no greater than that obtained with oral vaccine alone.抄録全体を表示PDF形式でダウンロード (1063K)
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