巻号一覧
16 巻 (1966)
- 6 号 p. 229-
- 4-5 号 p. 121-
- 3 号 p. 69-
- 1-2 号 p. 1-
前身誌
16 巻, 1-2 号
選択された号の論文の5件中1～5を表示しています
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谷口 武1966 年 16 巻 1-2 号 p. 1-5
発行日: 1966/04/30
公開日: 2010/03/16
ジャーナル フリーPDF形式でダウンロード (901K) -
平木 潔, 太田 善介, 三好 勇夫, 原田 英雄, 鈴木 信也, 六車 昌士1966 年 16 巻 1-2 号 p. 6-12
発行日: 1966/04/30
公開日: 2010/03/16
ジャーナル フリーSpontaneous mammary carcinoma of AKR mice and Xray-induced mammary carcinoma of C58 mice, both known as low-cancer strains, were investigated by electron microscopy.
In AKR mammary carcinoma, intracytoplasmic type A and extracellular type B particles were present. In C58 mammary carcinoma, extracellular type C particles were observed. In both tumors, mature virus particles, i.e. type B and type C particles, were formed by the process of budding of cytoplasmic membrane of carcinoma cells and present in the lumina of the milk ducts. Aberrant form of type C particles, which contained a filamentous structure instead of a nucleoid was also seen in C58 mammary carcinoma.
In serial in vivo transplantation of those carcinoma cells to the same strain mice, these virus particles were always present and formed from carcinoma cells, although the number of virus particles reduced almost successively with transplantations in AKR mammary carcinoma.
In tissue culture of C58 mammary carcinoma cells, type C particles similar to those in the original tumor were observed extracellularly. On the contraty to the presence of type B particles in the original AKR mammary tumor, however, two tissue culture strains derived from in vivo transplanted AKR carcinoma cells contained type C particles. These type C particles were conceivable to be the leukemia virus that naturally harbored in AKR mice and happened to have been tranferred to the tissue culture probably through its latent infection in carcinoma cells.抄録全体を表示PDF形式でダウンロード (4336K) -
宮本 包厚1966 年 16 巻 1-2 号 p. 13-31
発行日: 1966/04/30
公開日: 2010/03/16
ジャーナル フリーBrains of mice infected with the street rabies virus were observed by the electron microscope and by the light microscope for aiming to elucidate the origin of the Negri body. Adjacent thin (ca 0.05μ) and thick (ca 1.0μ) sections were prepared from Epon embedded blocks of the nerve cell band of Ammon's horn in the hippocampus where Negri bodies are apt to be abundant and by mapping the sections could be identified the same nerve cells including Negri bodies under both electron and light microscope.
The Negri bodies were recognizable by light microscopy as lightly colored cytoplasmic inclusions containing few dark granules after toluidine blue staining. Electron micrographs of the consecutive sections revealed that the ground substance of the Negri body was identical with the matrix which was commonly related to rabies virus replication of both street and fixed strains. This evidence follows the conclusion that the degree of capability to produce Negri bodies depends upon the formation of numerous and extensive foci of virus synthesis.
It might appear that Negri bodies increase in their volume by coherence of neighbouring small Negri bodies within the same cytoplasm, resulting in varied positions of inner bodies within large Negri bodies. The inner body, an important constituent of the Negri body, being surrounded by the ground substance of this inclusion was composed of virus particles associated with some amount of cytoplasmic constituents.抄録全体を表示PDF形式でダウンロード (12596K) -
松本 昇1966 年 16 巻 1-2 号 p. 32-40
発行日: 1966/04/30
公開日: 2010/03/16
ジャーナル フリーThe fine structure of inclusion bodies and the morphological changes of VERO cells (monkey kidney cell line) infected with Edmonston strain of measles virus were examined in the electron microscope and localization of viral antigen was followed by means of ferritin-conjugated antibody method. And the following results were obtained.
1) In the nuclear inclusion area, randomly arrayed tubular structures with frequent ordered arrays were recognized. The cytoplasmic inclusion was also consisted of an aggregate of randomly arrayed tubular structures. The tubular structure in the cytoplasmic inclusion was twice wider than that in the nuclear inclusion, having approximate diameter of 30 to 40mμ. The exact nature of these tubular material remains undetermined.
2) Many of the infected cells which contained cytoplasmic inclusion developed a lot of tiny cytoplasmic processes and spherical bodies on and outside the cell wall. In general, most of the spherical bodies had dense outer membrane but they were varied in size, shape and internal structure.
3) Measles virus antigen was demonstrated on the surface of the spherical bodies and cytoplasmic processes. It was hardly possible to identify mature virus particle because the spherical bodies were markedly pleomorphic. The spherical bodies and cytoplasmic processes with dense outer membrane containing viral antigen would be intimately related to the maturation and release of measles virus.抄録全体を表示PDF形式でダウンロード (6004K) -
1966 年 16 巻 1-2 号 p. 41-62
発行日: 1966/04/30
公開日: 2010/03/16
ジャーナル フリーPDF形式でダウンロード (6238K)
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