巻号一覧
18 巻 (1968)
- 6 号 p. 475-
- 5 号 p. 345-
- 4 号 p. 281-
- 3 号 p. 205-
- 2 号 p. 111-
- 1 号 p. 1-
前身誌
18 巻, 4 号
選択された号の論文の6件中1～6を表示しています
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松本 稔1968 年 18 巻 4 号 p. 281-294
発行日: 1968/08/31
公開日: 2010/03/16
ジャーナル フリーPDF形式でダウンロード (2291K) -
第1報 力価及び抗原性の異るワクチンによる抗体産生桂田 光彦1968 年 18 巻 4 号 p. 295-304
発行日: 1968/08/31
公開日: 2010/03/16
ジャーナル フリーA number of junior high school students living in northern Hokkaido, where Japanese encephalitis (JE) is not prevalent, were inoculated with inactivated JE virus vaccines different in potency and antigenicity to study the production of antibody by the vaccination per se.
Following results were obtained;
1) The three lots of mouse brain Nakayama vaccines differed greatly in their antibody-producing capacities.
The capacity was the highest in the Lot 3 vaccine, the next highest was the Lot 2, and the lowest was Lot 1.
2) The antibody-producing capacity of the two JaGAr type vaccines, the mouse brain JaGAr and the tissue cultured Mukai, was remarkably lower than that of any lot of the three Nakayama vaccines. However, considerable antibody increase was achieved even by the JaGAr type vaccines, when the vaccines were inoculated twice with different intervals of time.
3) The booster effect on antibody response of the mouse brain Nakayama vaccine inoculated one year after the first vaccination was striking.
The antibody thus produced was maintained as high as 1:40 to 1:2560 even after one year of the booster vaccination.
4) The presence in serum of minimal amount of neutralizing antibody produced by initial vaccination stimulated greatly antibody production following subsequent vaccination. This emphasizes that the efficacy of JE vaccine for man should be investigated for those persons living in the area free of the virus.
5) Inoculation of the Nakayama vaccine produced antibody to heterologous JaGAr strain as well as to homologous Nakayama strain.
The heterologous antibody thus produced was one nineth to one-sixteenth in the titer of homologous antibody. This implies that the Nakayama antibody of 1:160 or higher in titer may be necessary to prevent infection with JaGAr type virus.
6) The neutralizing antibody obtained by the vaccination increased proportionally with the rise in hemagglutination-inhibition (HI) antibody, but the former antibody below 1:80 in titer could not be detected by the HI test.抄録全体を表示PDF形式でダウンロード (1269K) -
第2報 ワクチン接種により産生された抗体の物理化学的性状桂田 光彦1968 年 18 巻 4 号 p. 305-309
発行日: 1968/08/31
公開日: 2010/03/16
ジャーナル フリーInactivated Japanese encephalitis virus vaccine (Nakayama, Lot 3) was injected to persons free of serum neutralizing antibody against the virus, first in order to elucidate the development of serum antibody in the early stage after the vaccination, and secondly to study the physico-chemical properties of antibodies produced at different time after the vaccination.
The results are summarized as follows:
1) The neutralizing antibody appeared in sera from the 7th to 10th day post-vaccination. The titer reached its maximum on about the 20th day and gradually reduced thereafter.
2) The neutralizing antibody in sera collected within 3 weeks after vaccination was highly sensitive to the treatment with 2-mercaptoethanol (2-ME), while that collected on the 40-50th day was found to be somewhat insensitive to the treatment. In contrast to this, the antibody resistant to 2-ME was demonstrated in sera obtained on the 60th day post-vaccination.
3) Antibody in those sera were analyzed by means of gel filtration with Sephadex G-200. The serum collected on the 14th day post-vaccination contained only the 19S macroglobulin antibody. The serum on the 22nd day contained a small amount of the 7S antibody, in addition to the larger amount of remaining macroglobulin antibody.
Gel filtration of the 41st-day's serum showed a relative decrease in the amount of the 19S antibody, in contrast to an increase in the 7S antibody.
In serum collected one year after vaccination, persistence of minimum amount of macroglobulin antibody was demonstrated, though the majority of the neutralizing activity was contained in the 7S antibody.
A striking rise in neutralizing titer in serum following revaccination made one year after the primary injection was found to be due to an increase in the 7S antibody.
4) Evidence was obtained to indicate that the treatment with 2-ME reduced the activity of 19S antibody to one-eighth of its original titer, while that of the 7S antibody was no more affected by this reagent.抄録全体を表示PDF形式でダウンロード (630K) -
伊沢 久夫1968 年 18 巻 4 号 p. 310-316
発行日: 1968/08/31
公開日: 2010/03/16
ジャーナル フリーInvestigations into the viral susceptibility of hog cholera (HC) virus-carrier cell lines which were previously established from kidneys of pigs with experimental HC, demonstrated somewhat unique cytopathic changes of a Newcastle disease (ND) viral strain on the certain HC virus-carrier cultures. The cytopathic changes were characterized by formation of massive fusions of cells and vacuoles.
Preliminary observations on the unique cytopathic changes revealed that the changes occurred only when one of three ND viral strains, Sato strain, was exposed to two lines of the HC virus-carrier cultures which were originated from the same cell line. The cytopathic changes, however, were negative when the same ND viral strain was inoculated into remaining five cell cultures. In addition, exposure of the remaining two ND viral strains to the total of seven cell cultures including the above 2 lines of HC virus-carrier cultures did not result the unique cytopathic changes.
Evidence was shown that the cytopathic changes were not due to contaminant but due to Sato strain itself, especially to its infectivity.抄録全体を表示PDF形式でダウンロード (3091K) -
大塚 悟, 真子 憲治, 那須 正義, 青山 友三1968 年 18 巻 4 号 p. 317-322
発行日: 1968/08/31
公開日: 2010/03/16
ジャーナル フリーJE virus was isolated using suckling mice from the blood and other various organs of a new-born piglet, together with still-born fetuses, which were delivered from naturally infected mother sow on 24th of July in 1967. In addition, Fluorescent antibody technique (FAT) was used for detect the viral antigen with in the tissue. The results obtained are as follows:
1) JE virus was isolated from the blood, central nervous system (CNS), thymus gland, salivary gland and the lung in the new-born slaughtered within 10 hours after birth. The virus was found to 103.0-3.5 BLU (50% baby mouse lethal unit) of the CNS, 102.3 BLU of thymus and salivary gland, and <101.0 BLU of the lung and the blood. In addition, similar results of the virus isolation were observed in another one of same litter, which died immediately after birth. These data apparantly indicate that pig could be born with viremia following the viral infection in utero.
2) The HI antibody titers of the mother sow bled the next day of the delivery were 1:640. The antibody was found to be 2-ME (2-mercaptoethanol)-sensitive and it is assumed that the infection took place several weeks before delivery. Those of the new-born were undetectable (below 1:10).
3) The application of FAT was tested for the detection of the viral antigen in various tissues (except salivary gland) of the new-born. The viral antigen was demonstrated only in the nerve cells of the CNS and some kind of cells in the thymus. In the nerve cells, the viral antigen was observed exclusively in cytoplasm of them.抄録全体を表示PDF形式でダウンロード (1519K) -
三沢 洋1968 年 18 巻 4 号 p. 323-332
発行日: 1968/08/31
公開日: 2010/03/16
ジャーナル フリーIn this paper, an immunohistochemical study on the tumor antigen (T) induced by adenovirus type 12 infection to hamster embryo cells (HaE) in vitro was explored by the use of immunofluorescence technique. The infected cells were subjected to observe any antigenic alteration of T antigen stained with fluorescein isothiocyanate conjugated anti-T antigen hamster antibody after treatment of those cells with the following various reagents.
The antigenicity of T antigen was somewhat deteriorated by treatment with both proteolytic and carbohydrate-digesting enzymes and completely abolished with sodium periodate, phenol, deoxycholate, and sulfuric acid. However, it resisted to treatment with formaldehyde and organic solvents such as ethyl-ether, carbontetrachloride chloroform, while some hydrophilic organic solvents, for instance, acetone, methyl- and ethylalcohol acted somewhat deterioratively.
Interestingly, preabsorption of the conjugate with lactose resulted in inhibiting the positive fluorescence in T antigen
From these results, an assumption was made that adeno-12 induced T antigen in HaE would mainly consist of a protein which contains a part of polysuccharide like lactose as an immunodeterminant group.抄録全体を表示PDF形式でダウンロード (2373K)
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