巻号一覧
19 巻 (1969)
- 6 号 p. 311-
- 5 号 p. 177-
- 4 号 p. 111-
- 2-3 号 p. 65-
- 1 号 p. 1-
前身誌
19 巻, 2-3 号
選択された号の論文の5件中1～5を表示しています
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三舟 求真人, 林 薫1969 年 19 巻 2-3 号 p. 65-69
発行日: 1969年
公開日: 2010/03/16
ジャーナル フリーThe incorporation of 3H-uridine in the cellular RNA of uninfected PS cells was inhibited by adding 2μg/ml of actinomycin D. Monolayers of PS cells were infected with sindbis virus at 50 of moi and after 60 minutes of adsorption, 2μg/ml of actinomycin D was added. With the aid of plaque techinique, it was demonstrated that the replication of sindbis virus was unaffected by the presence of actinomycin D. For the investigation of the incorporation of 3H-uridine in the viral RNA, pulse labeling of sindbis virus infected PS cells was made by adding 3H-uridine at different incubation. Even in the presence of actinomycin D, grains based on the incorporation of 3H-uridine had reached maximal numbers at 6 hours after infection and it was always demonstrated in the cytoplasm of infected PS cells as seen in Figure 3 and Photo. No. 2.抄録全体を表示PDF形式でダウンロード (1246K) -
田波 洋, 山田 喜紹, 田崎 忠勝1969 年 19 巻 2-3 号 p. 70-76
発行日: 1969年
公開日: 2010/03/16
ジャーナル フリーThe rate of adsorption of a psittacosis agent (Goat Pneumonitis agent, strain Nagano) by monolayers of Earle's L cell have been investigated. It has been shown experimentally that the rate at which psittacosis agent elementary bodies (0.35μ diamater) arrive at a surface of the cell monolayers can be accurately predicted from the theory of Brownian motion. Proportion of particles adsorbed by the monolayers during an adsorption time of t seconds, Yt, is given as Yt=1.51×10-3√t.
There are good agreement between theoretical adsorption rate calculated from the above formula and experimentally obtained values within the experimental error. Therefore, we can estimate the number of total infective particles in the inoculum from the number of “infected particles” as shown by counting “inclusion froming cells” microscopically.
Adsorption of the psittacosis agent by L cell monolayers requires adequate concentration of cations. Optimum salt concentration for adsorption is approximately 10-2 M. Divalent cations (Ca2+, Mg2+ Mn2+) accelerate adsorption more effectively than univalent cations such as Na+, K+ NH+4 etc. Optimum pH of the medium for psittacosis adsorption has been found to be 6.1; between 5.5 and 6.5 there is a slight decline in the efficiency of adsorption. At either sides of these pH values adsorption rate declines markedly.抄録全体を表示PDF形式でダウンロード (916K) -
I. 濃縮と部分精製井上 栄1969 年 19 巻 2-3 号 p. 77-82
発行日: 1969年
公開日: 2010/03/16
ジャーナル フリーIt was confirmed that Japanese encephalitis (JE) virus, which belongs to arbo B group viruses, produced high titer interferon (IF) in mouse brains when it was inoculated intracerebrally.
The by-product (20% ethanol supernatant) of JE vaccine of alcohol-protamine (AP) method also contained IF activity, and mouse IF was highly concentrated from large volume of the by-product by zinc acetate and ammonium sulfate precipitation and was 1, 100 foldly purified with the aid of CM-Sephadex and hydroxylapatite column chromatography.抄録全体を表示PDF形式でダウンロード (751K) -
II. インターフェロン投与によるマウスvirus感染の阻止井上 栄1969 年 19 巻 2-3 号 p. 83-88
発行日: 1969年
公開日: 2010/03/16
ジャーナル フリーCrude or concentrated interferon (IF) solution from mouse brains infected with Japanese encephalitis virus (JEV) was administrated into mice before or after virus inoculation.
LD50's in the mice which were intraperitoneally pretreated with IF, then intraperitoneally infected with vesicular stomatitis virus (VSV), JEV and mouse hepatitis virus (MHV), were decreased 30-300 foldly compared with those in the control mice which had taken no IF pretreatment.
In the gronp of mice which had been infected with MHV (5LD50/mouse) and to which IF administration was begun at 4hrs post infection, mortality was reduced to 50% whereas all mice died in the covtrol group to which no IF was administrated.
As an in vitro model experiment for therapy against viral infection; onto the L cell monosheets on coverslips which had been infected with VSV (m.o.i. <<1), was added IF containing maintenance medium. And the process of inhibition of virus spreading to neighbouring cells and of “curing” of the monosheets from CPE was traced by staining with anti-VSV fluorescent antibody.抄録全体を表示PDF形式でダウンロード (1819K) -
1969 年 19 巻 2-3 号 p. 89-102
発行日: 1969年
公開日: 2010/03/16
ジャーナル フリーPDF形式でダウンロード (2004K)
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