巻号一覧
22 巻 (1972)
- 4 号 p. 215-
- 3 号 p. 107-
- 1-2 号 p. 1-
前身誌
22 巻, 1-2 号
選択された号の論文の12件中1～12を表示しています
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建部 到1972 年 22 巻 1-2 号 p. 1-13
発行日: 1972/07/01
公開日: 2010/03/16
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第1報 高張溶液に継代可能な HeLa 細胞の作製およびその生物学的性状渡辺 実1972 年 22 巻 1-2 号 p. 14-22
発行日: 1972/07/01
公開日: 2010/03/16
ジャーナル フリーIt has been reported that virus reproduction was inhibited when osmotic pressure decreased in maintenance medium (Eaton and Scala, 1956; Tolskaya et al., 1966), while no literature concerning hypertonic effect has appeared as yet, because no cells resistant to high osmotic pressure are available.
This paper presents an attempt made to produce a HeLa cell line that would be cultivated in hypertonic medium for a long time in order to examine hypertonic maintenance medium effect for on virus reproduction.
1) HeLa cells cultivated by the conventional method were suspended in a hypertonic growth medium containing twice as much NaCl as the normal medium does. Then, most of the cells (97-98%) were destroyed. The cells that survived, however, retained the multiplying potential. While repeated medium changes were continued, they started to grow in the hypertonic growth medium a few days later. After several serial cultivations the cells manifested constant growth in the hypertonic medium.
2) These HeLa cells were designated as HeLa-O200 cells (since their osmotic pressure was 200%). They were more bulky than, and about 3 times as heavy as, conventionally cultivated HeLa cells. They contained almost the same concentration of NaCl as the latter cells.
3) HeLa O200 cells lost the propagating capacity when subcultures were carried out over 30 to 60 times in the high osmotic medium. They ceased growing when they formed a monolayer. When they hypertonic medium was replaced by an isotonic medium, the cells increased again in number.
4) When HeLa-O200 cells were cultivated in the isotonic medium over two generations, they lost not only their resistance to high osmotic pressure but also their morphological and propagating characteristics. They did not appear to be the newly established cell line which had been selected for the first hypertonic cultivation.抄録全体を表示PDF形式でダウンロード (4816K) -
第2報 Poliovirus に対する感受性渡辺 実1972 年 22 巻 1-2 号 p. 23-30
発行日: 1972/07/01
公開日: 2010/03/16
ジャーナル フリーHeLa cells serially cultivated in the hypertonic growth medium (HeLa-O200 cells) were examined for susceptibility to polioviruses. Then they were used to investigate influences of hypertonic maintenance medium on virus production and cytopathic effect.
1) The high osmotic pressure of the hypertonic maintenance medium had little effect on the reproduction of the Mahoney and LSc, 2 ab strains, and on the cytopathic effects of the strains used in the present studies, except the Leon, 12 ab strain. The cytopathic effect of the Leon, 12 ab strain was inhibited to some degree in this medium.
2) The cytopathic effects of polioviruses appeared more slowly on HeLa-O200 cells than on HeLa cells. The attenuated strains revealed lower CP titers on HeLa-O200 cells at the final observation. The virulent strains varied in behavior from one strain to anoter. The Mahoney strain showed equal CP titers on both cell cultures at the final observation, although the titers determined on HeLa-O200 cells on the 1st and 2nd days were about 2 log lower than those on HeLa cells.
The infective titer of the MEF-1 strain estimated on HeLa-O200 cells was always lower than that on HeLa cells, as was seen in the case of the attenuated strains. The titer of the Saukett strain estimated on HeLa-O200 cells varied with the lot of cell culture used for estimation.
3) In plaque assay the Mahoney strain showed results similar to those obtained by the tube titration. There was no difference in plaque formation between the two types of cells.
The titer in PFU of the LSc, 2 ab strain determined on HeLa-O200 cells was a little lower than that on HeLa cells, but the difference in PFU titer was not so apparent as that in tube titration between HeLa cells and HeLa-O200 cells.
4) When HeLa-O200 cells were cultivated in the isotonic medium over 2 generations, they reached the same level of susceptibility to polioviruses as HeLa cells.
It was considered that the susceptibility of HeLa-O200 cells to polioviruses might have been altered not genetically but physiologically by the hypertonic cultivation.抄録全体を表示PDF形式でダウンロード (1054K) -
第3報 コクサツキーBウイルスおよびエコーウイルスに対する感受性渡辺 実1972 年 22 巻 1-2 号 p. 31-37
発行日: 1972/07/01
公開日: 2010/03/16
ジャーナル フリーThe hypertonic maintenance medium (MM) was examined for influence on virus reproduction and cytopathic effects of Coxsackie B and Echo viruses in experiments with HeLa cells serially cultivated in the hypertonic growth medium (HeLa-O200 cells). The susceptibility of these cells to the viruses was also studied.
1) In the tube titration all the types of Coxsackie B and Echo viruses revealed lower CP titers in the hypertonic MM than in the isotonic MM.
2) In the isotonic maintenance medium the CP titers of the viruses determined on HeLa-O200 cells were lower than those on HeLa cells. The difference, however, was not so apparent as that observed in comparative titration between the hypertonic and isotonic MM.
3) The greatest influence of the hypertonic MM on CP titer was observed with Coxsackie B-2 and Echo-7 viruses. The CP titers of these viruses were 5-6 log lower in the hypertonic MM than in the isotonic MM.
4) These viruses grew equally well in both maintenance media. The influence of the hypertonic MM on cytopathic effect did not appear to result from the inhibition of virus reproduction.
5) When the hypertonic MM was prepared with sucrose for the tube titration of Coxsackie B-2 virus, it was proved to have a mild inhibitory action over the cytopathic effect. The fact denotes that the inhibition of cytopathic effect was not brought about merely by the physical hypertonic condition, but required an increased in electrolyte (Na+).
6) Addition of cystine and glutamine to the hypertonic MM had no influence on the CP titers of the viruses.抄録全体を表示PDF形式でダウンロード (905K) -
第4編 家畜の日本脳炎ウイルス感染の血清学的ならびに気象学的研究山田 堅一郎, 橋本 信夫, 金光 正次1972 年 22 巻 1-2 号 p. 38-47
発行日: 1972/07/01
公開日: 2010/03/16
ジャーナル フリーSero-epizootiological studies were performed on Japanese encephalitis (JE) in wild and domestic animals in Hokkaido. The relation of climatic factors to JE outbreaks was also investigated.
1. Hemagglutination-inhibiting (HI) antibodies against both Nakayama and JaGAr-01 strains were found in 9 (1.5%) of 593 swine sera collected in different parts of Hokkaido in 1970. All the 9 sera were among those collected in the southern part (Hiyama district) of Hokkaido. Two of them were senitive to 2-ME.
2. A total of 12 pools of mosquitoes belonging to Aedes vexans nipponii, Culex pipiens pallens, and Anopheles sinensis were collected in the the Hiyama district. No virus was isolated from any of them.
3. Neutralizing antibody to the JaGAr-01 strain was detected from 48 (7.8%) of 604 bovine sera collected in different parts of Hokkaido by means of the microtiter method. The positive rate decreased gradually northward and became higher in accordance with the advance in age.
4. Sera were collected from 35 wild birds, 87 rodents, and 28 bats in Hokkaido in 1970. All of them were negative for the microneutralization test with the JaGAr-01 strain.
5. Climatic conditions for effect were investigated on the outbreak of JE in domestic animals by using the ten-day records of air temperature and precipitation over a period from April to October. An epizootic of JE occurred in a year when the mean ten-day temperature was 20°C or higher in summer, although it varied in size with the duration of the mean temperature. Precipitation was also found to affect JE infection of pigs in Hon shu, but such was not clearly demonstrated in Hokkaido.抄録全体を表示PDF形式でダウンロード (1525K) -
森 良一, 北原 靖久, 堀江 昭夫1972 年 22 巻 1-2 号 p. 48-54
発行日: 1972/07/01
公開日: 2010/03/16
ジャーナル フリーVirological and pathological studies were performed on newborn mice which had been infected experimentally with Coxsackievirus A16 and sacrificed serially every day.
Virus titers began to increase in skeletal muscles on the 3rd day after virus inoculation and became remarkably high on the 4th and 5th days. They increased little in the heart muscle on the 4th and 5th days, but not at all in any other organ or tissue examined, i. e., liver, spleen, kidney, pancreas, brain, or thymus.
Pathological changes were remarkable in the striated skeletal muscles especially on the 3rd and 4th days postinfection. Electron microscopic studies revealed the breakage of muscle bands and the increase of membranous systems in the cytoplasm. Slight changes were also found in the heart muscle but no changes at all in any other organ or tissue.抄録全体を表示PDF形式でダウンロード (9259K) -
高木 康至, 嘉儀 隆, 牛山 六男1972 年 22 巻 1-2 号 p. 55-57
発行日: 1972/07/01
公開日: 2010/03/16
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1972 年 22 巻 1-2 号 p. 58-80
発行日: 1972/07/01
公開日: 2010/03/16
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多ケ谷 勇1972 年 22 巻 1-2 号 p. 81-82
発行日: 1972/07/01
公開日: 2010/03/16
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水谷 裕迪, 清水 明1972 年 22 巻 1-2 号 p. 83-84
発行日: 1972/07/01
公開日: 2010/03/16
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ウイルスの構造1972 年 22 巻 1-2 号 p. 85-95
発行日: 1972/07/01
公開日: 2010/03/16
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徳田 悟一1972 年 22 巻 1-2 号 p. 96-98
発行日: 1972/07/01
公開日: 2010/03/16
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