ウイルス

植物ウイルスのアブラムシによる伝搬

三沢正生

1974 年 24 巻 4 号 p. 291-299
発行日: 1974/12/31
公開日: 2010/03/16

DOIhttps://doi.org/10.2222/jsv.24.291

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ECHO-6分離株のヒト胎児 (肺および腎) 細胞通過ウイルスの血球凝集反応および血球凝集抑制反応に関する研究

松田宗之, 香取幸治, 宮原満恵, 橋爪淑子, 久保田憲太郎

1974 年 24 巻 4 号 p. 300-308
発行日: 1974/12/31
公開日: 2010/03/16

DOIhttps://doi.org/10.2222/jsv.24.300

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The hemagglutination (HA) of ECHO-6 virus and the utilization of hemagglutination-inhibition (HI) for diagnosis were investigated with virus isolates and sera from some patients involved in an epidemic of infection with this virus in Toyama in 1965.
1. MK-passed in virus showed a maximum hemagglutination and cytopathic effect (CPE) 5 or 6 days, and infectivity 2 days after inoculation, while HEL- or HEK-passed virus showed a maximum hemagglutination and infectivity only 1 or 2 days and CPE 3 days after inoculation.
2. The loss of hemagglutinating activity and, in general, quite a distinct variation in HA titer were observed among such strains as passed through MK cell cultures. Only 28 strains (58.33%) out of 48 showed positive HA after 3 passages in MK cell culture. In contrast, no loss of HA was observed among strains passed through HEL or HEK cell cultures. The rate of HA-positive strains increased gradually with the advance in passage. Finally only 2 out of the 48 strains were negative for HA after 3 passages in HEL or HEK cell culture.
3. The mean HA titer after 3 passages in MK cell culture was only 3 times that of the primary culture, whereas that after 3 passages in HEL or HEK cell culture was 15 times.
4. Fifteen HA-positive and 3 HA-negative strains were identified as ECHO-6 virus by the hemagglutination-inhibition and the neutralization and the neutralization test, respectively.
5. The hemagglutination-inhibition test with patient's sera was a useful method for diagnosis in the early stage, since relatively high HI titers were observed in convalescent sera.

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5-BROMODEOXYURIDINE 処理したヒト白血病細胞内に見いだされた「電子密度の高い粒子」について

田波洋, 山田喜紹

1974 年 24 巻 4 号 p. 309-320
発行日: 1974/12/31
公開日: 2010/03/16

DOIhttps://doi.org/10.2222/jsv.24.309

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In an electron microscope study, it was demonstrated that particles resembling the type C particles had been produced in peripheral leukemic cells or leukocytes obtained from five patients with leukemia and one with reticulum cell sarcoma when these cells had been exposed to 5-bromodeoxyuridine in a short-termed culture. They had electron-dense sphere cores surrounded by a single nuit membrane and varying from 60 to 100nm in outer diameter. Fairly frequently, they appeared in clusters in intracytoplasmic vesicles formed in leukemic myelogenous cells or leukocytes. They were found neither in untreated leukocytes nor in drug-treated lymphocytes or eosinocytes obtained from the patients.
Similar electron-dense particles were also demonstrated in peripheral myelogenous leukocytes obtained from three of 10 healthy persons examined after exposure to the drug under the same experimental conditions as mentioned above. No biological activity of these particles was clarified.

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第10回日本ウイルス学会北海道支部総会

1974 年 24 巻 4 号 p. 321-326
発行日: 1974/12/31
公開日: 2010/03/16

DOIhttps://doi.org/10.2222/jsv.24.321

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第8回日本ウイルス学会シンポジウム

ミクソウイルスの生物学

1974 年 24 巻 4 号 p. 327-390
発行日: 1974/12/31
公開日: 2010/03/16

DOIhttps://doi.org/10.2222/jsv.24.327

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