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Volume 30, Issue 1
Displaying 1-7 of 7 articles from this issue
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Minoru MATUMOTO1980 Volume 30 Issue 1 Pages 1-10
Published: June 01, 1980
Released on J-STAGE: March 12, 2010
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Tsuneo MORISHMA1980 Volume 30 Issue 1 Pages 11-19
Published: June 01, 1980
Released on J-STAGE: March 12, 2010
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Tadashi YAMASHITA1980 Volume 30 Issue 1 Pages 21-31
Published: June 01, 1980
Released on J-STAGE: March 12, 2010
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Mitsuo SAKOH, Takashi NAKAYAMA, Katsutoshi AIHARA, Tetsuya OKA, Saneo ...1980 Volume 30 Issue 1 Pages 33-40
Published: June 01, 1980
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSIn 1977 to 1978 winter, mixed epidemic was observed in Kyushu district with H3N2 and H1N1 subtypes of influenza virus. Fourteen strains of influenza virus were isolated from an outbreak in the pediatric ward of a hospital. Hemagglutination with 13 strains was inhibited by anti-A/FM/1/47 (H1N1) serum but not by anti-A/Kumamoto/22/76 (H3N2) and by anti-A/Tokyo/1/77 (H3N2) sera, suggesting H1 subtype. However, hemagglutinin from one strain (MF/11) was inhibited neither by H1 nor H3 subtype serum. Acute serum from the case from which MF/11 strain was isolated showed negative HI to both H1 and H3 antigens. His convalescent serum showed significant rise in HI against both H1 and H3 antigens. An immune mouse serum against MF/11 strain strongly reacted with both H1 and H3 antigens in HI. MF/11 immune serum neutralize A/Tokyo/1/77 virus in high titer, and neutralize A/USSR/92/77 in lower extent. Above findings suggested coexistence of H3 and H1 antigens in MF/11 strain. Sixty four clones were obtained from plaques on MDCK monolayer inoculated with MF/11 strain at 3rd egg passage. Among 64 clones, 60 was identified as H3 and remained 4 as H1 in HI tests. Further in neuraminidase-inhibition tests carried out in 16 clones, 7 clones were identified as H3N2 and 2 were as H1N1. Remained 7 clones were finally determined as recombinants, 6 having H3N1 and 1 having H1N2 characters. Whether those recombinants were produced in man or in eggs during 3 passages is remained unknown.View full abstractDownload PDF (1046K) -
Haruhiko MACHIDA1980 Volume 30 Issue 1 Pages 41-46
Published: June 01, 1980
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSAmong several rat cell lines tested, an established cell line, JTC-19, derived from rat lung tissue was suitable for the titration of rat interferon. Although both a plaque reduction method and a microtiter technique (cytopathogenic effect reduction method) were employed satisfactorily for the titration of rat interferon, the assay by the former was more sensitive than that by the latter. Interferon induced in plasma of rat injected with poly (I)·poly (C) was about twofold less active in L cells than in JTC-19 cells. Whereas mouse interferon produced by poly (I)·poly (C)-treated L cells was much less active in JTC-19 cells than in L cells. Interferon activity was not detected in plasma 1hr after intraperitoneal injection of 2.5mg of poly (I)·poly (C) per kg. Although individual variation was found in titers of plasma interferon at each time point, the interferon titer rapidly increased within 2hr after the injection, reached a peak at 4 to 6hr, and then gradually decreased in all rats tested. Interferon was induced in rats by administration of Newcastle disease virus as well as poly (I)·poly (C). On the other hand, interferon-like activity was not detected in plasma of rat, strain SD, at 8, 16 or 24hr after injection of OK-432 or PS-K. The benefits of employing rats for interferon induction are as follows: 1) multiple bleedings from an individual animal with elape of time are possible with easy technics, 2) from the average of interferon titers in individuals of each group, different groups could be statistically compared in their productivity of interferon. In fact, we could get the time courses of plasma interferon level in individual rats and could show statistically significant difference in the interferon productivity of the three strains of rat; strain SD>Wistar>Fischer in order.View full abstractDownload PDF (783K) -
Ryoichi KOGASAKA1980 Volume 30 Issue 1 Pages 47-59
Published: June 01, 1980
Released on J-STAGE: March 12, 2010
JOURNAL FREE ACCESSRecently, it has been reported that human rotavirus is a major causative agent of acute non-bacterial diarrhea in infancy. This report presents a new finding on the Ultrastructure of fecal rotavirus by the aid of the electron microscopic examinations. Human rotavirus has a characteristic icosahedral structure, which has a honeycomb-like appearance on the surface of the smooth particles and has 42 hollow and isolated capsomers in the rough particles. The pentagonal capsomers are arranged at 12 apexes of the icosahedron. Thirty hexagonal capsomers are arranged at the center of each edge of each plane of the icosahedron. Subunits, possibly bridge-like units, connect the polygonal capsomers at the corner and hexagonally devide the intracapsomeric spaces. The absence of the intracapsomeric subunits transforms the smooth particles into the rough particles. Core is spherical and is located in inner space of the virion. There are some morphological differences between human rotavirus and human reovirus (type 3) cultured in the L cells.View full abstractDownload PDF (8410K) -
1980 Volume 30 Issue 1 Pages 61-72
Published: June 01, 1980
Released on J-STAGE: March 12, 2010
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