The binding activity of purified and
125I-labeled recombinant human interferon alpha-2a (rIFN alfa-2a) to FL cells was studied. The number of receptors per cell and the binding constant were estimated as 1.7×10
3 and 9.1×10
10 M
-1, respectively. These values are similar to those reported for natural human interferon alpha (nIFN alfa) and for recombinant human interferon alpha-2b. Unlabeled rIFN alfa-2a and unlabeled nIFN alfa produced in human leukocytes inhibited the binding of
125I-labeled rIFN alfa-2a to a similar degree.
These results suggest that rIFN alfa-2a binds to the same cell-receptors as those for nIFN alfa, and that the binding activities of the two are similar.
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