Effects of disinfectants to periphytic algae in glass tubes (7 mm in diameter, 50 cm in length) were examined using 2 types of media; ozonated medium and chlorinated medium. The periphytic algae used in this study were collected from natural fresh waters of Zaru River and were left for their attachments for 2 months in a vessel in which glass tubes were set. Incubation experiments using these tubes and 2 types of media were carried out for 30 days.
The time course experiments showed that chlorophyl a (chl.α) concentration of glass tube wall both in control medium and ozonated medium increased on the 9th day. The average chl.α concentration of three tube wals from the 12th to the 30th day were 10.2μg/cm
2 (control), 11.6μg/cm
2 (ozonated) and 7.4μg/cm
2 (chlorinated) respectively. No marked increase in the concentrations of chl.α in effluent of chlorinated medium were found. The average chl.α concentration in eflluent of the control and ozonated medium were almost the same (2.1 and 1.8μg/
l) in the first 13 days, and that of ozonated medium on the 17th day was about 3.5 times higher than the control. The maximum concentration of chl.α was 17.0μg/
l (ozonated) on the 26th day.
Among the algal species identified (42 in total),
Scenedesmus spp.,
Homoeothrix janthina and
Chroococcus spp. dominated. Different algal communities were formed on the walls of glass tube in chlorinated medium. Numerical abundance of
Chroococcus spp. was more than 50% on chlorinated glass tube walls from the 6th day.
Nitzschia palea in ozonated effluent incerased in the 15th day, accounting for 74.2%in the 25th day.
The present results showed that chlorination to the medium made a negative effect on the algal flora and standing crop on the glass wall, resulting in the marked change of species composition using cluster analysis and by ozonation algal biomass and species diversity increased.
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