Establishing a treatment process for practical, economic disposal of photo-processing waste (PW) has become an urgent environmental concern under recently enacted revisions of LondonTreaty. This paper describes the acclimation of sulfur-oxidizing bacteria (SOB), and the PW treatment performance by acclimated SOB in conjunction with granular activated carbon (GAC), which demonstrates as an effective biological treatment method.
No distinct difference in acclimating SOB to strong dilution of PW (50-times to 12-times dilution) was observed between two acclimation methods, i.e., using diluted PW directly and using ONM medium firstly to accumulate SOB. Comparison of SOB/GAC and SOB acclimation systems for further acclimation (10-times to 3-times dilution), demonstrated that SOB/GAC systemsucceeded in acclimating SOB to 3-times diluted PW, while SOB system failed in even 6-times dilution. Meanwhile, culture of SOB in various dilutions of calcination PW system and inorganic salts of PW composition system clarified that SOB can grow even on solution of raw PW containing 12% salinity.
Weak dilution of PW (5-times to 4-times dilution) was treated by using SOB in conjunction with 10 g/
l GAC in a long-term (98 d) continuous treatment (HRT=1.9-7.7d) such that mass-reduced sulfur compounds were completely oxidized to sulfate, while biodegradable organics in dissolved organic carbon (DOC) were simultaneously degraded at a removal rate of 55%, andresulted in 68% removal rate in chemical oxygen demand (COD) .
Selective culture of the SOB sludge by using seven kinds of medium revealed that about 90% of the SOB was facultative chemoautotrophic bacteria and also two kinds of heterotrophicbacteria were present.
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