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Volume 37 (2001)
- Issue 4 Pages 135-
- Issue 3 Pages 93-
- Issue 2 Pages 55-
- Issue 1 Pages 1-
Volume 37, Issue 2
Displaying 1-5 of 5 articles from this issue
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NORIO SUGIURA, NORIO IWAMI, TOMOAKI ITAYAMA, YUHEI INAMORI2001 Volume 37 Issue 2 Pages 55-61
Published: June 15, 2001
Released on J-STAGE: December 09, 2010
JOURNAL FREE ACCESSIn order to reduce favorably the growing Microcystis aeruginosa in eutrophicated lakes and marshes with no defective influence to aquatic ecosystem, suppression characteristics of M. aeruginosa in a model pond combined with bioreactor, in which predative microanimals, Monas guttula, Aeolosoma hemprichi and Philodina erythrophthalma were inoculated, was continuously examined. M. aeruginosa in the pond decreased slowly at early 2 days running, after the 4th day the reduction was clearly accerelated under hydraulic retention time of 8 hrs. For quantitative evaluation of the biomass-reduction, the reduction rate of M. aeruginosa in the lake was simulated by that of bioreactor with high densed micro-animals on the carrier. It was found that practical reduction rate of M. aeruginosa was ten-fold higher than theoretical value. As a reason for the significant reduction, it was considered that micro-animals released from the bioreactor combined with the lake might contribute the reduction.View full abstractDownload PDF (880K) -
TAKAYUKI NISHIO, YASUHIRO FUJIWARA, TARO YOSHIKURA, ISAO FUKUNAGA2001 Volume 37 Issue 2 Pages 63-68
Published: June 15, 2001
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe relations between proliferation of phytoplankton and changes in water quality wereexamined to control the water quality in the lagoon of a sea-based dredged soil disposal site.
Correlation of Chl. α with BOD and COD was high, and it was implied that organic pollution was mainly caused by the primary production in the lagoon when hydraulic retention time become long. Because high pH of the wastewater was a problem, factors that influence pH were analyzed. The results of the simulation of pH changes in the lagoon from IC concentration was fairly reasonable; however, changes in the IC concentration was only partly explained by the proliferation of phytoplankton. Factors that influence IC concentration other than proliferation of phytoplankton remained unresolved.View full abstractDownload PDF (667K) -
SEIICHIROU KUROKI, MASAAKI YOSHIYAMA, KENJI FURUKAWA2001 Volume 37 Issue 2 Pages 69-75
Published: June 15, 2001
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSA purple nonsulfur bacteria, strain K-3, was isolated from photodependent denitrifying sludge (PDDS) that was cultivated by fill and draw using methanol as H-donor fbr denitrificalion. It was identified as Rhodobacter sphaeroides by physiological examines and 16S rRNA analysis. With illumination, Strain K-3 has the capability to grow under both aerobic and anaerobic conditions. However, strain K-3 does not have nitrate reduction capability. Additionally, strain K-3 cannot utilize methanol which is the main carbon source of PDDS. These factors suggest that strain K-3 interacts with other bacteria in PDDS in nutrient cycling. While strain K-3 in pure culture can degrade acid azo dyes With hght exposure, it cannot degrade acid azo dyes under dark conditions. Additionally, the presence of enzymes that can degrade dye chemicals under light conditions was discussed.View full abstractDownload PDF (830K) -
NAOHIRO NODA, YOSHITAKA EBIE, HAJIME IKUTA, SATOSHI TSUNEDA, AKIRA HIR ...2001 Volume 37 Issue 2 Pages 77-86
Published: June 15, 2001
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSMonoclonal antibody method is very useful and helpful for in situ detection of the specific bacteria like nitrifiers in a mixed bacterial habitat such as a biofilm. In this study, the specificities of monoclonal antibodies raised against Nitrosomonas europaea (IFO14298) and Nitrobacter winogradskyi (IFO14297) were checked by sandwich ELISA. As a result, it was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria and specificity for various genus and species of nitrifying bacteria. The spatial distribution of N. europaea and N. winogradskyi immobilized by entrapped-type and attached-type PEG gel pellet introduced into the nitrification reactor was examined by in situ fluorescent antibody technique. As a result, it was found that N. winogradskyi was more sensitive to the damage during entrapping process than N. europaea. Whereas N. winogradskyi existed only near the surface area, N. europaea existed near the surface area and at inner part of gel pellet. On the other hand, in attached-type PEG gel pellet, the high density areas of N. winogradskyi were observed compared with entrapped-type PEG gel pellet. It was suggested that this fluorescent antibody technique was utilized for understanding the microbial community of nitrifying bacteria as a quick and favorable tool.View full abstractDownload PDF (4312K) -
AKIRA HIRATA, AMI AMINAH MEUTIA, SATOSHI TSUNEDA2001 Volume 37 Issue 2 Pages 87-92
Published: June 15, 2001
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSStructural examinations of a biofilm immobilized on small cement ball (CB) particles in a three-phase fluidized bed reactor for a highly concentrated nitrification process were carried out. With transmission and scanning electron microscopes, a diverse microbial arrangement was determined. The scanning electron micrographs showed that a large number of clustering bacteria was covered by extracellular polymers. Between the clusters, many open spaces were found. The transmission electron micrographs showed a regular arrangement of bacteria inside the biofilm. Most clusters of Nitrosomonas sp.-like bacteria were found near the surface of the biomm and the majority of Nitrobacter sp.-like bacteria were observed inside the biofilm. At least 14 types of bacteria were found in the mixed culture nitrifying biofilms.View full abstractDownload PDF (7434K)
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