Screening of fungi having ability for decolorization of dye was carried out by using Remazol brilliant blue R, a kind of dye, as an indicator. Twenty three fungi having ability for the decolorization of the dye were found by the screening from 195 samples of decayed woods and soils. Among them, three fungi named 563, V 1, and V 2 had higher ability for the decolorization of the dye than those of other fungi separated by the screening. Microbial degradations of 2, 7-Dichlorodibenzo-p-dioxin (2, 7-DCDD) by the three fungi (563, V 1, and V 2) separated by the screening and by three wood-rotting fungi having ability for biodegradation of lignins (
Phanerochaete chrysosporium, Coriolus versicolor, and
Fusarium solani) were conducted. The six fungi degraded 24.1% to 83.1% of 2, 7-DCDD. The maximum rate of degradation of 2, 7-DCDD was obtained by fungus V 2 when the incubation was conducted for 15 days after addition of 1.25 m Mol of 2, 7-DCDD to culture medium of each of six fungi. The rate of the degradation was 61.8%. In the case of the 30 day's incubation in the same concentration, the maximum rate of the degradation was obtained by
P. chrysosporium. The rate of the degradation was 65.8%. On the other hand, the maximum rates of degradation of 2, 7-DCDD were obtained by fungus V 2 when the incubations were conducted for 15 and 30 days, respectively, after addition of 0.25 m Mol of 2, 7-DCDD to culture medium of each of six fungi. The rates of the degradation were 83.1% and 79.9%, respectively. In addition, a part of 2, 7-DCDD was incorporated into each mycelium of six fungi during the incubation. The rate of degradation of 2, 7-DCDD increased with increase in the lignin peroxidase activity in extracellular crude enzymes solution from six fungi.
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