Recently, an increasing social awareness of environmental issue has prompted administrative guidelines and regulations of industrial effluent. In this situation, simultaneous pursuit of environmental preservation and improvement of productivity is important for the pulp and paper industry. For stable operation of a plant, it is essential to enhance capability of wastewater treatment. In this report, we propose two effective and convenient approaches for improving wastewater treatment. The first is the application of microbial products in activated sludge, so-called “Bioaugmentation”, and its tracking technique. Microscopic FISH (Fluorescence in situ hybridization) analysis of bacterial cells allows for “remodeling” of activated sludge by the microbial products. The second is the analysis method for monitoring physiological activity of bacterial community in activated sludge. Visualization of the physiological activity of bacterial community, which is invisible by conventional light-microscopic observation, should provide a useful indicator for controlling activated sludge treatment. In addition, we show an example of improvement of activated sludge treatment in pulp and paper plant, in which microbial products and fluorescence imaging techniques were used.
Since invention of activated sludge system, it has been unclear how bacterial composition and its physiological state shifts in this “blackbox”. In our approach reported here, a remarkable feature is that we can monitor a part of the inside of “blackbox”, and can improve the system efficiently.
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