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Volume 18 (1999)
- Issue 4 Pages 323-
- Issue 3 Pages 189-
- Issue 2 Pages 139-
- Issue 1 Pages 113-
Volume 18, Issue 1
Displaying 1-4 of 4 articles from this issue
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Masa-Aki Okubo, Akira Matsuno, Masayoshi Namba, Hiroshi Hosoya1999 Volume 18 Issue 1 Pages 113-117
Published: March 31, 1999
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSThe mH1 monoclonal antibody, which specifically recognizes microvillus projections of dividing HeLa cells, was characterized. Distribution of mH1 antigen in dividing HeLa cells was assayed by colloidal gold immunoelectron microscopic observation. Counting of gold particles in immunoelectron micrographs suggests that the mH1 antigen constantly distributes at microvillus projections of whole cell surface of dividing cells. In immunofluorescence experiments, the mH1 stained the surface of types of human culture cell lines including another cervical cancer, hepatoma, ovarian carcinoma, normal fibroblast, and transformed fibroblast cell lines, but did not specifically stain their cleavage furrows as in HeLa cells.View full abstractDownload PDF (1397K) -
Kazuhiro Chida, Hirotake Kitaura1999 Volume 18 Issue 1 Pages 119-123
Published: March 31, 1999
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSWe examined possible solvents for DNA which can be directly introduced into the nuclei of cultured cells by physical microinjection through glass capillaries. DNA of the plasmid pCH110containing lac-Z, a β-galactosidase gene, or the plasmid pSV2neo containing ned, a neomycinphosphotransferase gene, suspended in solvents commonly used in the fields of cell biology and biochemistry, such as PBS, TBS, or distilled water alone, was microinjected into the nuclei of NRK cells. Transient expression of the lac-Z gene and induction of neomycin-resistance occurred almost at the same level for all these solvents, except for EDTA-containing solutions, e. g., TE. For TE solution, the transient and stable expression levels were reduced, and could be recovered by supplying magnesium ions. Furthermore, we examined the effects of EDTA on the macromolecule barrier of nuclear pores. Following EDTA injection, fluorescein-conjugated albumin, that does not ordinarily pass through nuclear pores freely, immediately leaked from the nucleus of injected cells into the cytoplasm. These results indicate that microinjection of EDTA into a nucleus disrupts nuclear pore function, possibly resulting in inhibition of transient and stable expression of introduced genes.View full abstractDownload PDF (1516K) -
Hisaaki Saeki, Toshio Kudo1999 Volume 18 Issue 1 Pages 125-129
Published: March 31, 1999
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSThe amplification of short tandem repeat (STR) loci using the polymerase chain reaction (PCR) with four fluorescent primers was applied for discrimination of cultured cell lines maintained in our cell bank. PCR methods using the GammaSTRTM multiplex system (D16S539, D7S820, D13S317, D5S818) were applied to 63 human cell lines to determine how practical this approach is for routine purposes. Amplified DNA extracted from cells demonstrated one or two DNA bands on SDS-PAGE (4% gel containing urea) for each cell line, allowing their easy identification in 56 of the cases. The remaining 7 cell lines demonstrated common bands. Since the gel patterns obtained by PCR using the STR multiplex primers were very clear, and the method is time-saving, this approach can be recommended as a good cell identification system for quality control of human cell lines.View full abstractDownload PDF (1327K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...1999 Volume 18 Issue 1 Pages 131-138
Published: March 31, 1999
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESS
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