TISSUE CULTURE RESEARCH COMMUNICATIONS

[title in Japanese]

1999 Volume 18 Issue 3 Pages 189-200
Published: September 30, 1999
Released on J-STAGE: November 13, 2012

DOIhttps://doi.org/10.11418/jtca1981.18.3_189

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[title in Japanese]

1999 Volume 18 Issue 3 Pages 203-228
Published: September 30, 1999
Released on J-STAGE: November 13, 2012

DOIhttps://doi.org/10.11418/jtca1981.18.3_203

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[title in Japanese]

1999 Volume 18 Issue 3 Pages 229-251
Published: September 30, 1999
Released on J-STAGE: November 13, 2012

DOIhttps://doi.org/10.11418/jtca1981.18.3_229

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[title in Japanese]

1999 Volume 18 Issue 3 Pages 253-285
Published: September 30, 1999
Released on J-STAGE: November 13, 2012

DOIhttps://doi.org/10.11418/jtca1981.18.3_253

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A TRANSPLANTED AND CULTURED CELL LINE ESTABLISHED FROM CANINE MALIGNANT HEMANGIOPERICYTOMA AND ITS IMMUNOHISTOCHEMICAL CHARACTERIZATION

Kikumi OGIHARA, Saori MIYATAKE, Seigo KISHIKAWA, Tokio TSUCHIYA, Yukia ...

1999 Volume 18 Issue 3 Pages 291-300
Published: September 30, 1999
Released on J-STAGE: November 13, 2012

DOIhttps://doi.org/10.11418/jtca1981.18.3_291

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It is generally believed that hemangiopericytoma (HP) arises from Zimmermann's pericyte, although its origin and tumor categorization remain unestablished. In the present study, a transplanted and cultured cell line was established from canine hemangiopericytoma (CHP). The cell line showed a variety of morphologies including small N/C ratio of cells, multinucleated cells, spindle-shaped cells, bipolar and multipolar cells in addition to fibroblast-like cells. The doubling time of cells in culture was approximately 32 hours, when 1.9×10⁵ cells per ml were inoculated at the 51st subcultivation. lmmunohistochemical analysis showed that endothelial cells and pericytes were positive to actin, while the fibrosarcoma-like cells were positive to vimentin. Chromosome numbers in CHP cell line at the 35th passage in culture distributed in a wide range of 31 to 100 with a modal number of 79. Based on the findings obtained, tumor cells grown in in vitro cultivation and transplanted in nude rats showed almost the same histological feature as that of the original tumor, indicating that the tumor cells established in the present study may be useful as a model of CHP.

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Differential cytotoxicity of anticahcer agents in hMutSα-deficient and -proficient human colorectal cancer cells

Iichiro UCHIDA, Xiaoling ZHONG

1999 Volume 18 Issue 3 Pages 301-312
Published: September 30, 1999
Released on J-STAGE: November 13, 2012

DOIhttps://doi.org/10.11418/jtca1981.18.3_301

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Mismatch repair (MMR)-deficient cells exhibit drug resistance to several anticancer agents including N-methyl-AP-nitro-N-nitrosoguanidine (MNNG), cisplatin, and adriamycin. Since these agents are potent mutagens, it is possible to select resistant clones of tumor cells during chemotherapy. Prior to determining whether drug cytotoxicity was altered by MMR-deficiency, mutation in the (A)₈ repeat region of the hMSH3 gene of the MMR-deficient human colorectal cancer cell line HCT116 and the MMR-proficient human chromosome 3-transferred HCT116(HCT116+ch3) was comfirmed. A screening method was then determined using MNNG cytotoxicity in both cell lines and 20 additional anticancer agents were examined. Clonogenic cytotoxic assay revealed in 8 anticancer agents (streptozotocin,5-fluorouracil, tegafur, bleomycin, mitomycin C, vinblastine, vincristine, and nidoran) maintaining the desired level of cytotoxicity required a higher concentration in HCT116 than in HCT116+ch3. Cytosine β-D-arabinofuranoside, chlorambucil, and epirubicin were more cytotoxic to HCT116. Dacarbazine, nitrogen mustard,3'-azido-3'-deoxythymidine, aclarubicin, neocarzinostatin, actinomycin D, and peplomycin possessed similar cytotoxicity. These results suggest that drugs with higher or uncompromised sensitivity can circumvent drug resistance due to MMR-deficiency in tumor cells.

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

1999 Volume 18 Issue 3 Pages 313-316
Published: September 30, 1999
Released on J-STAGE: November 13, 2012

DOIhttps://doi.org/10.11418/jtca1981.18.3_313

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

1999 Volume 18 Issue 3 Pages 317-321
Published: September 30, 1999
Released on J-STAGE: November 13, 2012

DOIhttps://doi.org/10.11418/jtca1981.18.3_317

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