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Volume 20, Issue 1
Displaying 1-4 of 4 articles from this issue
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Isao Nozaki, Masayoshi Namba2001 Volume 20 Issue 1 Pages 1-3
Published: March 31, 2001
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSBy transfection of an expression vector of human cytochrome P450 2E1 (CYP2E1) into a human hepatoma cell line (HLE), a new cell line (HLE/2E1) that stably expresses activity of CYP2E1 has been established. The HLE/2E1 cell line expressed a higher level of CYP2E1 mRNA than did the mother HLE cell line. CYP2E1 enzyme activity determined by a p-nitrophenol oxidation assay was also higher in HLE/2E1 cells than in HLE cells. In addition, the enzyme activity of the HLE/2E1 cells was increased by ethanol treatment. Exposure to acetaminophen (APAP)caused a greater decrease in viability of the HLE/2E1 cells than that of the HLE cells, as determined by the MTT assay. The cytotoxicity of APAP to HLE/2E1 cells was enhanced by 24-h preincubation of HLE/2E1 cells with ethanol. These results show that this cell line provides a useful model for studying catalytic properties of CYP2E1 and cytotoxic mechanisms of chemicals metabolized by CYP2E1.View full abstractDownload PDF (666K) -
Yasuko Shirai2001 Volume 20 Issue 1 Pages 5-13
Published: March 31, 2001
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSPersonal comments were described about the procedures of informed consent in the process of donation to human, tissue bank in National Children's Hospital, based on legal, ethical, psychological, and social considerations about genetic diagnosis and counseling for muscular dystrophy. There are obvious differences between 'to receive medical care' and 'to be a subject of medical studies', since the former pursues patient's best interest but the latter aims scientific progress in medicine. In the present study and expected other human tissue banks, the samples are originally removed by the purpose of medical care. The authors of the original paper are required to explain an appropriateness of another standard for donation of human tissues.View full abstractDownload PDF (2227K) -
Yasushi Yukinari2001 Volume 20 Issue 1 Pages 15-18
Published: 2001
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSPersonal comments were described about the pilot study of human tonsil bank in National Children's Hospital. Main feature of the present study was that donors were infants and children. Much more discussion will be needed for the tissue donation from minors. Basically, donors should be persons who can comprehend the explanation by adults, i. e., school children or greater. Although inclusive explanation is impracticable at the time of informed consent, more concrete presentation of expected use of tissues, such as outline of previous research is desirable. Although Japanese government announced several guidelines concerning life science research, including research on human embryonic stem cells, the discussions during the development of guidelines were not really sufficient. Since various kinds of human tissues will be handled by human tissue bank, nationwide mutual discussion must be necessary.View full abstractDownload PDF (749K) -
Shin Enosawa, Seiichi Suzuki, Hiroshi Amemiya, Nobuaki Tsuchihashi, No ...2001 Volume 20 Issue 1 Pages 19-26
Published: March 31, 2001
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSWe described reply to comments by Shirai Y, Yukinari Y, and others, on our previous report about the pilot study of human tissue research resource bank (see http: //cellbank. nihs. go. jp/jtca/ethics/tonsil. htm). The main points were involoved in (1) informed consent with children, and (2) how to explain the expected purpose of use of human tissues. The former is involved in not only children but also patients whose condition is physically and mentally uneasy just before operation, and patients who are not fully able to understand. Taking these comments into consideration, we discussed again the problems to organize the system for human tissue bank.View full abstractDownload PDF (1760K)
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