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Volume 25 (2006)
- Issue 3-4 Pages 129-
- Issue 2 Pages 105-
- Issue 1 Pages 23-
Volume 25, Issue 2
Displaying 1-3 of 3 articles from this issue
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Ken SUGO, Machiko KATO, Tsuyoshi ISHIKAWA, Akira YAMAMOTO, Tetsuro OGA ...2006 Volume 25 Issue 2 Pages 105-111
Published: June 30, 2006
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSWe developed and characterized hydroxyapatite (HA) microcarrier for high-density cell culture. HA microcarrier was prepared by coating the surface of polymer (nylon, epoxy and polyethylene)beads with HA particles. The mixtures of polymer beads and HA were processed with a Mechanofusion system. Nylon beads with HA showed the most suitable coating efficiency, and the specific gravity, according to scanning electron microscopy and a gas pycnometer. HA microcarriers with four different composition ratios were prepared from mixtures of nylon beads and HA at weight ratios of 100:0.5, 100:1, 100:2 and 100:3, to determine the optimum mixture ratio. Vero cells were cultured by use of these HA microcarriers. The HA microcarrier with a weight ratio of 100:1 was the most suitable cell carrier bead, because of its specific gravity, and the effective ability for Vero cells to proliferate on it.View full abstractDownload PDF (1836K) -
Ken SUGO, Machiko KATO, Tsuyoshi ISHIKAWA, Akira YAMAMOTO, Tetsuro OGA ...2006 Volume 25 Issue 2 Pages 113-118
Published: June 30, 2006
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSWe developed a hydroxyapatite (HA) microcarrier prepared by coating the surface of nylon beads with HA particles. Cell growth ability and viability were observed on the newly produced HA microcarrier (CELLYARD beads) and compared with that on a commercially available dextran microcarrier (Cytodex 1). Madin-Darby canine kidney cells (MDCK) and Vero cells were cultured using CELLYARD beads and Cytodex 1 in a 100 mL spinner flask. At 5days of incubation, MDCK cells proliferated on CELLYARD beads (2.31 × 106 cells/mL) effectively, at about 1.6 times the cell densities for Cytodex 1 (1.44 × 106 cells/mL). Vero cells also proliferated on CELLYARD beads (8.40 × 105 cells/mL) effectively, almost equal to that for Cytodex 1 (8.85 × 105 cells/mL). MDCK cells and Vero cells harvested from CELLYARD beads had much higher viability than those from Cytodex 1. CELLYARD beads had equal or better performance, compared with Cytodex 1, and would be a good material for high-density cell culture.View full abstractDownload PDF (1580K) -
Ken MURATA, Mikiko KAWASAKI, Akihiro KATOH, Masakatsu TAKEUCHI, Tomoko ...2006 Volume 25 Issue 2 Pages 119-127
Published: June 30, 2006
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSFreshly isolated bovine oviduct epithelial cells (BOECs) were processed to long-term cultivation until the cell multiplication potential is limited in vitro. Replicative senescence is monitored by the expression of senescence-associated β-galactosidase and a significant reduction in sizes of telomere restriction fragment (TRF). Immortalized cell lines were isolated after transfecting SV40 large T or hTERT. Several independently isolated BOEC lines; BO+SV (a, b, c and d) and BO+TERT (a and b) were established as a clonal cell line that harbor SV40large T or activated hTERT. Since BO+SV lines maintained TRF without telomerase activation, these lines achieved immortality due to ALT (alternative lengthening of telomeres). In contrast, BO+TERT lines show progressive telomere elongation along with the strong telomerase activity. These results demonstrate that the immortalized BOEC lines by the oncogenic virus SV40 large T or telomerase catalytic subunit hTERT generated distinctive cell lines in which telomere erosion was protected by either ALT or the activated telomerase, respectively. Karyotype analysis showed that all immortal BOEC lines had a number of metacentric chromosomes resulted from acrocentric chromosome fusion. These BOECs lines may be useful for investigating various aspects of cytogenetics and cell genetics in terms of telomere dynamics and genomic instability.View full abstractDownload PDF (2689K)
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