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Volume 26 (2007)
- Issue 4 Pages 171-
- Issue 3 Pages 149-
- Issue 2 Pages 115-
- Issue 1 Pages 21-
Volume 26, Issue 4
Displaying 1-3 of 3 articles from this issue
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Nobuhiko MARUNOUCHI, Toshimasa SAKAKIBARA, Mahito OHKUMA, Eiichi MIYAC ...2007 Volume 26 Issue 4 Pages 171-183
Published: 2007
Released on J-STAGE: March 13, 2008
JOURNAL FREE ACCESSBone marrow stroma cells (MSCs) have been shown to differentiate into multiple lineages and have great potential for regenerative therapy. We have obtained hMSCs from 6 human adults. They were all positive for CD13, CD44, and CD90 and weakly positive for CD49, while negative for CD45, suggesting that they were different from hematopoetic stem cells. hMSCs were induced to become neuronal cells and maintained as long as three weeks in the serum free medium supplemented with N2. An increase in the amount of mRNA was observed for the NeuroD1, neurofilament M and H, MAP2, neuron-specific enolase, tryptophan hydroxylase, Nurr1, and neuron specific Na+ channel genes, and the existence of voltage-gated Na+ channels that were sensitive to tetrodotoxin was confirmed electro physiologically. These results suggested that hMSCs differentiated into serotonergic neural cells. However, the expression of genes specific for stroma cells, the Big-h3 and vimentin-genes, was observed equally during the induction process, indicating that the expression pattern was not the completely same as in genuine neural cells. hMSCs cultured with serial passages showed aging phenomena at the cellular level under the various conditions examined in this study. Trials to isolate cellular clones proliferating indefinitely have not succeeded.View full abstractDownload PDF (1206K) -
Daisuke Ishimura, Naoki Yamamoto, Kaori Tajima, Osuke Washimi, Yasuhir ...2007 Volume 26 Issue 4 Pages 185-192
Published: 2007
Released on J-STAGE: March 13, 2008
JOURNAL FREE ACCESSWe analyzed the cell surface marker of Adipose-derived stromal vascular fraction culture cells (ADSVFs) derived from mouse subcutaneous adipose tissue. We used cell-aggregation or atelocollagen gel culture methods to differentiate ADSVFs to chondrocytes. We sorted CD105-positive cells from ADSVFs as adipose-derived stem cells (ASCs), and compared the differentiation efficiency to chondrocytes of both cells. As a result, the average of CD105 positive cells in ADSVFs were 65.1 ± 5.0%. Using the cell-aggregation culture method to differentiate ADSVFs to chondrocytes, we detected aggrecan mRNA by using cell of over 2 × 105 cells/ml. We detected the same level of aggrecan mRNA by cell-aggregation culture method using CD105-positive cells. When we assumed the regenerative medicine of the cartilage tissue on the clinical application, technique to culture chondrocytes in the shape of gel sheet matching the shape of cartilage defect area.View full abstractDownload PDF (1053K) -
Kamaljit KAUR, Nashi WIDODO, Avinash NAGPAL, Sunil C KAUL, Renu WADHWA2007 Volume 26 Issue 4 Pages 193-199
Published: 2007
Released on J-STAGE: March 13, 2008
JOURNAL FREE ACCESSMedicinal value of Withania somnifera Dunal (Ashwagandha) extends from anti-inflammatory, antiseptic, anti-stress, anti-arthritic, anti-rheumatic, anti-bronchitis, immuno- and appetite- stimulator to promote overall memory, rejuvenation, vitality, endurance, stamina and longevity. However, the molecular biology of myriads of these anti-disease or health-promoting effects remains insufficiently explored to date. We earlier reported that the Leaf extract from Ashwagandha (Lash) has selective anticancer activity that operates through the activation of p53 tumor suppressor pathway. Here we report that the selective killing activity of Lash can be employed in combination with anticancer drugs to yield an effective combinatorial anticancer formulation.View full abstractDownload PDF (669K)
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