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Volume 27 (2008)
- Issue 4 Pages 139-
- Issue 2+3 Pages 107-
- Issue 1 Pages 23-
Volume 27, Issue 4
Displaying 1-5 of 5 articles from this issue
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REVIEW
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Miho Kusuda Furue2008 Volume 27 Issue 4 Pages 139-147
Published: 2008
Released on J-STAGE: February 24, 2009
JOURNAL FREE ACCESSIn 1998, human embryonic stem (ES) cells have been established. Since then, the human ES cells have been used as a tool for understanding the mechanisms in human development and regeneration application research in the world. However, in Japan, not so many searchers have used human ES cells. It is caused from several issues. In 2007, human induced pluripotent stem (iPS) cells have been developed. The situation in Japanese stem cell research have been changing. As the characteristics of Human iPS cells are quite similar to those of human ES cells, researcher can use the protocols of human ES cells for iPS cell research. In this review, I have summarized the culture and characteristics of human ES and iPS cells.View full abstractDownload PDF (916K)
REGULAR ARTICLE
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Kimiko TAKAHASHI, Katsuyuki KOBAYASHI, Masako MITSUI, Tomotada ODAKA, ...2008 Volume 27 Issue 4 Pages 149-159
Published: 2008
Released on J-STAGE: February 24, 2009
JOURNAL FREE ACCESSPulmonary fibroblasts actively secrete PAI-1 at injured sites. KiF fibroblasts with the deletion allele of the PAI-1 promoter gene produced and secreted larger amounts of PAI-1 than WI-38 fibroblasts with the insertion allele. The effects of dexamethasone on PAI-1 expression after stimulation with TNFα were examined using these two human lung fibroblast lines. Dexamethasone initially reduced TNFα-induced NF-κB translocation, PAI-1 mRNA expression and PAI-1 secretion. The effects in KiF appeared earlier than in WI-38. However, longer treatment with dexamethasone eventually led to larger PAI-1 secretion than that observed in TNFa-unstimulated cells. These results suggest that the polymorphism of the PAI-1 promoter gene regulates not only PAI-1 production but also the response to dexamethasone.View full abstractDownload PDF (565K) -
Yasuhiro Yamamoto, Naoki Yamamoto, Kaori Tajima, Ayumi Ohno, Yuki Wash ...2008 Volume 27 Issue 4 Pages 161-168
Published: 2008
Released on J-STAGE: February 24, 2009
JOURNAL FREE ACCESSHuman multicentric osteosarcoma (HMOS) is a rare variant of osteosarcoma, and for which there is still no efficient treatment. We have recently established two cell lines from the primary lesion (humerus) and the later lesion (sternum) in patient with HMOS. Immunohistochemical staining for CXCR4/CXCL12 for these cell lines and surgical specimen of primary lesion (humerus) and another later lesion (left ilium). It is reported that CXCR4/CXCL12 chemokine/receptor axis plays a central role in cell migration in metastasis and dissemination of various cancers, and is associated with bone metastasis. Immunohistochemical findings indicated CXCR4 expression in left ilium lesion was stronger than that of humerus lesion, whereas, in both of cell lines, expression of CXCR4 and CXCL12 were observed, and especially, the increase of those expression was observed in the later lesion. These findings suggested that expression of CXCR4/CXCL12 axis is involved in pathogenesis of MOS, especially in the process of tumor cell metastasis.View full abstractDownload PDF (1108K) -
Takahiko Suzuki, Kiyoshi Miyagawa2008 Volume 27 Issue 4 Pages 169-175
Published: 2008
Released on J-STAGE: February 24, 2009
JOURNAL FREE ACCESSIn cultured cardaic myocytes, the uptake and utilization levels of fatty acids are very low compared with that of in vivo. Fatty acids are thought to be not only energy substrate but also a stimulator of peroxisome proliferation activated receptor (PPAR). In this study, the effect of stimulation of fatty acid uptake to the change of isoforms of myosin heavy chain (MHC) was investigated. The stimulation of oleic acid uptake by dipyridamole changes MHC isoform from immature type β to mature type α. This result suggests that the transporter of fatty acids are down regulated at the culture condition, and this may cause the expression of dedifferentiation phenotype of MHC isoform gene.View full abstractDownload PDF (747K)
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Yukio NAKAMURA2008 Volume 27 Issue 4 Pages 177-184
Published: 2008
Released on J-STAGE: February 24, 2009
JOURNAL FREE ACCESSThe Cell Engineering Division of RIKEN BioResource Center is a not-for-profit public “Cell Bank” that accepts donation and deposit of human and animal cell materials developed by life science research community. We examine, standardize, amplify, preserve, and provide cell materials to the interested scientists around the world. Recently, we have also started “Stem Cell Bank”. We are providing with human somatic stem cells such as the stem cells present in umbilical cord blood and the mesenchymal stem cells. A number of mouse and non-human primate embryonic stem (ES) cell lines are also available from our bank. In addition, we will provide with human ES cell lines in the near future. Dr. Yamanaka who has established induced pluripotent stem (iPS) cell lines kindly deposited mouse and human iPS cell lines in our bank. Mouse iPS cell line is already available, and human iPS cell lines will be available in the near future from our bank.View full abstractDownload PDF (1745K)
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