TISSUE CULTURE RESEARCH COMMUNICATIONS
Online ISSN : 1881-3704
Print ISSN : 0912-3636
ISSN-L : 0912-3636
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Volume 34, Issue 2
Displaying 1-5 of 5 articles from this issue
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ORIGINAL ARTICLE
  • Effect of nerve growth factor on three-dimensional culture of adult rat spinal tissue embedded in the hydrogel PuraMatrix
    Ai Kaneko, Yoshiyuki Sankai
    Article type: ORIGINAL ARTICLE
    2015 Volume 34 Issue 2 Pages 123-132
    Published: 2015
    Released on J-STAGE: July 19, 2016
    DOIhttps://doi.org/10.11418/jtca.34.123
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    Central nervous system (CNS) neurons in adult mammals display limited regeneration after injury, and poor functional recovery. Ex vivo three-dimensional organotypic tissue culture bridges the gap between monolayer in vitro cultures and the in vivo environment.
    In this study, adult rat spinal tissue explants were embedded in the three-dimensional nanofibrous hydrogel PuraMatrix and cultured in serum-free medium containing NGF. We also examined the long-term culture of adult CNS tissues in PuraMatrix for 8 weeks.
    25% and 50% PuraMatrix cultures resulted in 90% cell viability and 700-μm thick spinal tissues after 8 weeks, which were significantly higher (twice) than two-dimensional cultures. Cells migrated significantly further from rostral and caudal spinal tissues embedded in 50% PuraMatrix when cultured with NGF (~2 mm), compared with those without NGF. Spinal tissues cultured with 25% PuraMatrix exhibited the largest number of FM1-43-positive spots, but the highest frequency of tissue detachment from the well. From a viewpoint of easy handling and reproducibility of CNS regeneration in vivo, 50% PuraMatrix seemed to be the most suitable.
    Thus, our new culture system could be applied to numerous studies of CNS regeneration in vivo, including clinical effects of drugs, and chronic toxicity testing.
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SPECIAL CONTRIBUTION
  • IN MEMORY OF DR. ROBIN HOLLIDAY, HIS LABORATORY IN MID-NINETEEN EIGHTIES, AND TESTING DNA METHYLATION THEORY OF IN-VITRO CELLULAR AGING
    Toshiharu MATSUMURA
    Article type: SPECIAL CONTRIBUTION
    2015 Volume 34 Issue 2 Pages 133-138
    Published: 2015
    Released on J-STAGE: July 19, 2016
    DOIhttps://doi.org/10.11418/jtca.34.133
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    Robin Holliday, a noted British philosopher and a sculptor, born in 1932 died in 2014. After his remarkable contribution in nineteen sixties and seventies to modern molecular biology through his presentation of a genetic recombination model, i.e., Holliday model, and his proposal of epigenetic control mechanisms based on DNA methylation, he poured in nineteen eighties his scientific interests in the mechanisms of aging, particularly in solving the problem of in-vitro limited proliferative lifespan of cultured human diploid cells (HDCs). Two major findings obtained during that time in his laboratory were that cell’s memory of proliferative life span can be modified with a pulse treatment of DNA-methyl transferase inhibitors, 5-azacytidine and 5-azadeoxycytidine, and that SV40-infected-, but not immortalized, HDCs maintain their level of DNA methylation until the end of their proliferative life span. It was shown with these two lines of findings that the drift of DNA-methylation level is influential, but not essential determinant of the limitation of proliferative potential. These studies underlay further studies of Hayflick limit in nineteen nineties including the discovery of telomerase participation.
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REVIEW
  • Design of Bioactive materials for strictly regulating graft cells to cure the central nervous disorders
    Tadashi Nakaji-Hirabayashi, Kurumi Fujimoto, Chirag Gujral, Yuuta Mina ...
    Article type: REVIEW
    2015 Volume 34 Issue 2 Pages 139-151
    Published: 2015
    Released on J-STAGE: July 19, 2016
    DOIhttps://doi.org/10.11418/jtca.34.139
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    The therapy using cell transplantation has been focused as the potential method to completely cure intractable disorders. Especially, it has been said that the cell-based therapy is exclusive method to fully recover Parkinson’s disease because the cause of Parkinson’s disease is the denaturation and dropout of dopaminergic neuron in the striatum of the midbrain. From these backgrounds, various researches concerning the cell-based therapy of Parkinson’s disease using brain tissue of the fetus, neural progenitor cells derived from fetus tissue and pluripotent stem cells have been carried out. However, we found that the initial viability of transplanted tissue and cells is extremely low(~5%) and the transplanted cells cannot be received the regulation for regeneration of crushed tissue from the host. Therefore, our group aimed to develop a functional hydrogel for cell transplantation to protect the graft survival and control the grafted cells for neuronal regeneration.
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