1957年8月よりPMS注射によるめん羊の多産試験を行った. 過去の産歴により同一条件のものについて試験群と対照群に区分し, 発情予定前にPMSを注射して, その妊娠, 分娩および哺育の状態を観察した結果をとりまとめると次の通りである.
1. PMSの注射の時期は発情予定日4日前に行ったところ, 注射後翌日の1例を除いて, 2～5日目, 平均3.3日目に発情し受胎することができたので注射時期として, この時期が適当と思われる.
2. 注射に用いたPMSは生血清凍結乾燥粉末を蒸留水に溶解し, 水溶液として筋肉内注射したが, 母めん羊にはアレルギー反応その他の異常は認められなかった.
3. 双子の生れる率については, 対照群と試験群はそれぞれ29.4: 43.4%であった. 10%の危険率を認めれば両群の差は有意であるが, さらに例数を増加してたしかめる必要がある.
4. 産子数はPMSの注射量におおむね正比例するといわれるが, 多産のための実際上の応用量は500 IUが適当と思われる.
5. 在胎日数が試験群ではわずかに短縮したが, 雌雄の生れる割合, 産子の体重, 子畜生存数などは試験群と対照群の間に有意の差は認められなかった.

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In order to find the best method to concentrate microfilariae in the test blood, acetic acid, acetone, saponine, formalin, benzarconium hydrochloride, and distilled water were examined for hemolytic action. Also technics for concentrating microfilariae were investigated. As a result, the following method was proved to be the best.
Prepare a solution of:
Filtrated methylene blue (0.5%) 5cc
Acetone 5cc
Sodium citrate 0.2g
Water 90cc
1) Put 9 cc of the soltion in a graduated centrifuge test tube.
2) Add 1 cc of the blood drawn from the radial vein of a dog.
3) Invert the test tube several times to mix the contents, the open end being covered with a finger.(This procedure preserves the contents for a long period at room temperature.)
4) Centrifuge 1500 rpm for 10 minutes.
5) Remove the supernatant fluid with a ru-bber-bulb pipette, leaving the same quantity of fluid, usually 0.05cc, as the sediment.
6) Pick up 0.1 cc of the sediment plus the remaining fluid with a pipette.
7) Examine microscopically the mixture mo-unted on a 24×32 mm cover glass.
Microscopic observation:
As shown in the picture on p., the sediment consists of leucocytes and microfilariae. The microfilariae are in an extended position and are stained with methylene blue.
The authors'experience has confirmed that this method will detect any small number of microfilariae so long as they exist in a dog, and that it is superior to any other method previously used.

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Experimental studies were made on the quality of tomato juice agar, plate count agar, and delta's fuchsin lactose agar for the detection and count of lactic bacteria in fermented milk and acidmilk drinks.The results obtained are summarized as follows.
1) Tomato juice agar is an excellent plate medium for the detection and count of lactic bacteria.
2) Plate count agar, as well as tomato juice agar, has a good qualility to favor the growth of lactic bacteria.It is difficult, however, to differentiate lactic bacteria from others by use of this medium. When B. C. P. is added to this medium at the rate of 0.006%, lactic bacteria change the color of the indicator used around a colony to yellow. Accordingly, the differentiation of lactic from non-lactic bacteria can be made comparatively with case.
3) Lactic streptococci grow selectively on Delta's fuchsin lactose agar and the growth of lactic bacilli and other bacteria is controlled on this medium.
4) From the results mentioned above, tomato juice agar and B. C. P.-added plate count agar are recommended for the direct plate count of lactic bacteria and Delta's fuchsin lactose agar is available for distinguishing bacilli from cocci.
5) With these media, t he detection and count of the estimated number of lactic bacteria were carried out in 10 samples of fermented milk and acid-milk drinks and the following results were obtained.
In fermented milk, the largest number of lactic bacteria was 72×10⁹/cc and the smallest 63×10⁸/cc, while in acid-milk drinks, the largest number was 24×10¹⁰/cc and the smallest 26×10⁵/cc.
In fermented milk, 3 of 4 Yoghurt samples contained Lact.bulgaricus as starter, and the remaining one contained Lact. bulgaricus and Str. lactis. Only one sample of fermented milk contained Lact. acidophilus as starter. In acid-milk drinks, 2 of 5 samples contained. Lact. bulgaricus and the remaining 3, Lact. acidophilus as starter.

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Recently grievances and petitions have been aroused by the inhabitants of Tokyo that pharmaceutical destruction of stray and ownerless dogs be carried out as they have been threatening people and domestic animals as well during night. According to the Rabies Prevention Law, however, no pharmaceutical destruction of strayed dogs shall be enforced except during the period which is officially announced from a special need for preventing the spread of rabies and eradicating the infected dogs. Such being the case, it has become necessary to capture dogs in a state of anesthesia by allowing them to have bait containing anesthetic perorally.
Experiments were performed on 513 dogs captured and passing the legal period of detention, with bait of meat containing each of 8 proprietary anesthetics, pentobarbital, phenol-barbital, barbital, orthopan, evipan, sulfonal, chloral hydrate, and brobarin. When a dog was given the following anesthetics in such doses as mentioned, it was anesthetized in 30minutes and waked up in 6 hours, without suffering death.
Single preparation: pentobarbital, 0.2 to 0.5g.
Mixed preparations:(1) pentobarbital plus brobarin, (2) pentobarbital plus evipan, and (3) pentobarbital plus orthopan, 0.3 to 0.5g each.
The results indicate that this form of capture using anesthetic is available for practical purposes.

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Nephrectomy was performed experimentally on healthy dogs and goats, totaling 22 There are two methods for nephrectomy. One is carried out extraperitoneally and the other through the peritoneum. The purpose of operation will decide which method is to be adopted in a particular case. In general, the latter seems to be advantageous.
The animal is laid down on the operating table with its side to be operated up. A pillow is inserted under its loin so that the kidney may easily be exposed during operation. Disinfection and anesthesia are conducted as usual. An incision 7 to 12 cm in length is made on the skin near the posterior border of the last rib and the inferior border of the musculus quadratus lumborum and obliquely toward the inferior part of the crest of the ilium. Then the abdominal muscles and the peritoneum are incised to enable the operator's hand enter the abdominal cavity to search for the kidney. The peritoneum covering the anterior surface of the kidney is broken and the adipose tissue enveloping the organ teased by his fingers. When the kidney becomes free in the broken tissue, it is made to slide out into the peritoneal cavity with its stalk protected by two fingers of the operator from breaking. The renal artery and vein and the ureter are separated individually, ligated at two sites, and cut off between the sites. After the kidney is removed, the incised area is closed as usual.
As contamination is very little in this surgical operation, any after-treatment is scarcely needed until the wound is healed.
An important point in this operation is to take scrupulous care not to break blood vessels in the teasing of the adipose capsules, removal of the kidney into the abdominal cavity, and separation of the renal vessels and ureter. When a large amount of internal hemorrhage occurs by cutting a vessel, the opening of the incision on the abdominal wall is enlarge quickly so that the operator's hand may be inserted in the abdominal cavity and may press the abdominal aorta against the vertebra. At the same time the bleeding is checked temporarily by Pean's forceps. The blood is wiped away from inside the abdominal cavity. Then, by loosening Pean's forceps slightly, sites of hemorrhage are detected. The cut end of the renal artery which has been found is ligated firmly and the hemorrhage is successfully checked.

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