The experimental animals used consisted of 25 milch cows and one mare which had been diagnosed as endometritis from general clinical findings and the results of bacteriological examination and which had remained non-conceptive for a long time. The aim of the experiment was to promote the natural healing power of the animal which constitutes the basis of the mechanism of healing.
Investigation was performed on the side of the animal, as well as on the side of the bacteria involved, from a viewpoint of therapeutic treatment. For these purposes, without using concurrently any sulfa drug or antibiotic which had been employed conventionally in many cases, a cell-activating agent (karuchinomin, a proprietary product of the Toma Kaken Co., Ltd.) was administered alone. It was introduced into some uteri which had been washed with sterilized physiologic saline and others which had been intact without no cleaning treatment, in a does of 50 to 100 cc, the dose varying in accordance with the size of each uterus. In some experimental animals, this agent was injected, concurrently with the intra-uterine administration, into the gluteus muscle in a dose of 50cc. All the animals thus treated were held under continual observation. Generally speaking, turbidity disappeared from the mucus in the estrous period, the pH value of the mucus lowered, changes of the uterine wall decreased or disappeared, and the interval between the final treatrnent and artificial insemination was shortened in the treated animals, showing effective results.
With the advancement of chemotherapy, antibiotics have been abused and the fundamental treatment which promotes the natural healing power is apt to be overlooked. In the chemotherapy of endometritis which takes a chronic course, it seems to be necessary to select any suitable medicine by investigating the sensitivity of bacteria to such medicine and to take the function of any therapeutic agent activating the cells of the animal concerned into consideration.

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Bithionolの牛肝蛭に対する駆虫効果とくに適量を知るため, 各投薬量による剖検例, 野外駆虫試験例について効果判定を行なった結果をもとにして, 一律のper/kg薬量によらない補正投薬量を仮設し, これを適量として野外試験を行なった. これらの結果を要約すればつぎのようである.
1. Bithionol投薬牛の剖検による効果判定では, 35mg/kg以上で成熟肝蛭を完全に駆虫し, 投薬量の増加によっては幼若虫に対しても駆虫効力がある.
2. 虫卵検査による効果判定では15mg/kg2回投与で34%, 20, 30, 35mg/kgの各投薬量で, それぞれ43, 66, 88%の陰転率を示した.
3. 投薬適量の概略は600kgの牛に189, 500kgに16g, 400kgに149, 300kgでは129の1回飼料混与を標準とし, 寄生数および牛の状態によって19の幅を設けた. その結果, 肝蛭卵の陰転率は77%, 陰転しなかった牛ではいちじるしく虫卵数が減少した.
4. 投薬適量による副作用は下痢が16%, 食欲減退が13%に現われた, しかしこの副作用は軽微で短期間内に回復した.

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Nine outbreaks of food poisoning caused by Salmonella enteritidis occurred successively all over the old metropolitan area of Hyogo Ward and some part of Ikuta ward adjacent to it within the city limits of Kobe, Hyogo Prefecture, during 2 period of from June 22 to July 3, 1959. In these outbreaks were involved 34 persons, of whom 3 died. S. enteritidis was detected, at the rate of 100 per cent, from the fecal specimens collected from the persons involved. When 1, 128 of 9, 028 rats caught within the city limits during the same period as mentioned above were examined for bacteria, S. enteritidis was detected from 28 of 826 rats caught in and around the area where the persons involved had been reported (the rate of detection being 3.4 per cent). The same organism was detected from one of 302 rats caught in areas not related to that in which the involved persons had been found (the rate of detection being 0.3 per cent).
The isolated organisms were classified into two types, A and B, by means of phage typing. Type A was strongly virulent and type B weakly virulent for mice.
From he results of survey on the occurrence of patients involved in these outbreaks of food poisoning and those of detection of bacteria from rats caught in and around the related area, it was presumed that there had been an epizootic of S. enteritidis infection among rats in the area where the persons involved had been reported and that outbreaks of food poisoning had occurred intensively on account of the food contaminated by rats harboring the causative organisms in individual houses from which the patients were detected.

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Milk samples were collected separately from each quarter of the udders of 12 cows giving positive Brucella serum agglutination tests and subjected to the following tests:(1) pH test with B.T.B. solution, (2) Peroxydase staining by the Breed method (copper sulfate method) for regular and differential cell counts, (3) counting of Brucella organisms with serum agar and selective media, (4) general bacterial counts with sheep blood agar and tomato-juice agar, and (5) the determination of milk serum agglutination titers by the Weybridge method.
The results obtained from these tests are summarized as follows. As long as ordinary mastitis was making no progress, the milk secreted from a Brucella-infected quarter gave a total cell count of more than 500, 000 per cc, with peroxydasepositive cells which appeared at the rate of less than 50 per cent, mostly around 30 per cent, and had a pH value of more than 6.8. The total cell count ran parallel with the shift of Brucella organisms. Among peroxydase-negative cells, small round monocytes (corresponding to lymphocytes) occupied less than 50 per cent. Besides these, large mononuclear cells, especially those with elliptic or irregularshaped nuclei, made their appearance at a high rate.
These results seem to indicate that it is possible to detect an infected puarter of the udder by examining the number and kind of bacteria contained in the milk and that the degree of infection in such quarter is presumed from the results of successive observations on the quarter.

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ジステンパー鶏胎化ウイルスを用いた中和実験を, 種々の血清について検索し次の結果を得た.
1. イヌジズテソパーめ中和抗体は生後3～4ヵ月の仔犬ではほとんど認められなかったが, 生後1～2年の成犬では非常に高度にみとめられた.
2. ジステソバ鼠に臨床上罹患していると思われるイヌの中和抗体価は種々まちまちの価であった.
3. モルモットの腹腔内あるいはイヌの皮下に鶏胎化ジステンパー生ウイルスワクチン, あるいはホル々リン不活化臓器ワクチンを接種すると, モルモットでは接種後21日目をピークとして血清中和抗体の産生がみられ, イヌでは接種後14～42日目をピークとして非常に高度の中和抗体産生がみられた. またモルモットの場合には中和抗体は接種したウイルス含有量に比例した. なお臓器ワクチン接種ではいずれの場合も抗体の産生程度ははるかに低かった.
4. 市販抗ジステンパー免疫血清製剤, イヌ血清ガンマーグロブリン製剤中には高度の中和抗体が認められた.

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出産後寒さに向ったため, 室温は15～20℃を保たしめ, 直ちに父親ヒョウと母親ライオンを別離し, まったく秘密裏に仔レオポンを母親ライオンに一任し, 遂に38日目父親ヒョウと親仔水いらずの同居を決行し発表にいたったので, その間の苦労, 心配は言外のものであった. 以上のごとく昭和35年1月10日現在までは頗る健康 (もちろん12月20日に雌の風邪気味はあったが) であり, 将来への生長の希望を明るいものにしてくれているが, 1年経って見ないとまだまだ心配はつきない. これからの努力によって生長し, 3年以上経過した暁は雄のタテガミ発生にも希望はもてるし, F₂を誕生さすことによって動物遺伝学をくつがえして見たいという野望にももえている次第であります.

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